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The FMRFamide-like neuropeptide AF2 (Ascaris suum) is present in the free-living nematode, Panagrellus redivivus (Nematoda, Rhabditida)

Published online by Cambridge University Press:  06 April 2009

A. G. Maule ,
C. Shaw ,
J. W. Bowman ,
D. W. Halton ,
D. P. Thompson ,
T. G. Geary  and
L. Thim
A. G. Maule
Affiliation:
Comparative Neuroendocrinology Research Group, Schools of Clinical Medicine and Biology and Biochemistry, The Queen's University of Belfast, Belfast BT7 INN, Northern Ireland
C. Shaw
Affiliation:
Comparative Neuroendocrinology Research Group, Schools of Clinical Medicine and Biology and Biochemistry, The Queen's University of Belfast, Belfast BT7 INN, Northern Ireland
J. W. Bowman
Affiliation:
The Upjohn Company, Kalamazoo, MI 49001, USA
D. W. Halton
Affiliation:
Comparative Neuroendocrinology Research Group, Schools of Clinical Medicine and Biology and Biochemistry, The Queen's University of Belfast, Belfast BT7 INN, Northern Ireland
D. P. Thompson
Affiliation:
The Upjohn Company, Kalamazoo, MI 49001, USA
T. G. Geary
Affiliation:
The Upjohn Company, Kalamazoo, MI 49001, USA
L. Thim
Affiliation:
Novo Nordisk A/S, 2880 Bagsvaerd, Denmark
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Summary

Available primary structural information suggests that the FMRFamide-related peptides (FaRPs) from parasitic and free-living nematodes are different, and that free-living forms may not represent appropriate models for the study of the neurochemistry of parasitic forms in the laboratory. However, here we report the isolation and unequivocal identification of AF2 (originally isolated from the parasite, Ascaris suum) from acidified alcoholic extracts of the free-living species, Panagrellus redivivus. While reverse-phase HPLC analysis of extracts revealed FMRFamide-immunoreactivity to be highly heterogeneous, AF2 was the predominant FMRFamide-immunoreactive peptide present (at least 26 pmol/g wet weight of worms). This peptide was also the major immunoreactant identified by an antiserum raised to the conserved C- terminal hexapeptide amide of mammalian pancreatic polypeptide (PP), which has been used previously to isolate neuropeptide F (NPF). These observations were confirmed by radioimmunoassay and chromatographic fractionation of an acidified alcoholic extract of A. suum heads. The FMRFamide-related peptides present in a nematode extract may be highly dependent on the extraction medium employed, and these data would suggest that this complement of neuropeptides may not be as different between parasitic and free-living nematodes as initial studies have suggested. Finally, all of the evidence suggests that NPF is not present in nematodes and that the PP-immunoreactant previously demonstrated immunochemically is probably AF2.

Keywords

Ascaris suumPanagrellus redivivusNematodaFMRFamide-related peptidesAF2neuropeptide Fpeptide isolationpeptide sequencing
Type
Research Article
Information
Parasitology , Volume 109 , Issue 3 , September 1994 , pp. 351 - 356
Copyright
Copyright © Cambridge University Press 1994

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