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Effect of Zn on the adsorption and desorption of Cry1Ab toxin from Bacillus thuringiensis on clay minerals

Published online by Cambridge University Press:  09 July 2018

X. Y. Zhou ,
H. F. Liu ,
X. Z. Lu ,
J. C. Hao ,
L. L. Shi  and
Q. Hu
X. Y. Zhou*
Affiliation:
Tianjin Engineering and Technology Research Center of Agricultural Products Processing, Department of Food Science, Tianjin Agricultural University, Tianjin 300384, China
H. F. Liu
Affiliation:
Department of Agronomy, Tianjin Agricultural University, Tianjin 300384, China
X. Z. Lu
Affiliation:
Department of Agronomy, Tianjin Agricultural University, Tianjin 300384, China
J. C. Hao
Affiliation:
Department of Agronomy, Tianjin Agricultural University, Tianjin 300384, China
L. L. Shi
Affiliation:
Department of Agronomy, Tianjin Agricultural University, Tianjin 300384, China
Q. Hu
Affiliation:
Department of Agronomy, Tianjin Agricultural University, Tianjin 300384, China
*
*E-mail: zhouxueyongts@163.com
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Abstract

The influence of Zn on the adsorption and desorption of Cry1Ab toxin from Bacillus thuringiensis (Bt) on palygorskite and montmorillonite was studied. The adsorption of the toxin gradually increased with increasing Zn concentration from 0 to 1.0 mmol L–1, and then decreased with further increase in Zn concentration. The adsorption isotherms of the toxin in the absence and presence of Zn were well described by the Langmuir equation (R2 > 0.9810–0.9991). The separation factor (RL) decreased with increase of Zn concentration, suggesting that the irreversibility of the adsorption increases. The XRD results showed that the treatment by Tris buffer or Zn(NO3)2 solution caused an expansion of the interlayer space of montmorillonite but did not affect palygorskite. The IR spectra suggest that Zn was likely to be combined with amino groups on the surface of the toxin. The presence of Zn during the adsorption of the toxin decreased desorption, suggesting that the residual risk of toxin would be exacerbated if soil is polluted by zinc.

Keywords

zincCry1Ab toxinmontmorillonitepalygorskiteadsorptiondesorption
Type
Research Article
Information
Clay Minerals , Volume 48 , Issue 4 , September 2013 , pp. 605 - 612
Copyright
Copyright © The Mineralogical Society of Great Britain and Ireland 2013

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