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Effects of calcium-free ageing on ethanol-induced activation and developmental potential of mouse oocytes

Published online by Cambridge University Press:  22 May 2023

Chun-Hui Jin ,
Ren-Ren Chen ,
Xiu-Yun Feng ,
Jun-Gui Zhao ,
Ming-Tao Xu ,
Min Zhang ,
Jun-Zuo Wang  and
Jing-He Tan Open the ORCID record for Jing-He Tan [Opens in a new window]
Chun-Hui Jin
Affiliation:
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an City 271018, Shandong Province, China
Ren-Ren Chen
Affiliation:
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an City 271018, Shandong Province, China
Xiu-Yun Feng
Affiliation:
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an City 271018, Shandong Province, China
Jun-Gui Zhao
Affiliation:
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an City 271018, Shandong Province, China
Ming-Tao Xu
Affiliation:
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an City 271018, Shandong Province, China
Min Zhang
Affiliation:
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an City 271018, Shandong Province, China
Jun-Zuo Wang*
Affiliation:
Department of Reproduction and Genetics, Tai’an City Central Hospital, Tai’an, Shandong Province, China
Jing-He Tan*
Affiliation:
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an City 271018, Shandong Province, China
*
Corresponding author: Jing-He Tan. College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an City, Shandong 271018, China. Fax: +86 0538 8241419. E-mail: tanjh@sdau.edu.cn. Jun-Zuo Wang. Department of Reproduction and Genetics, Tai’an City Central Hospital, Tai’an, Shandong Province, China. E-mail: wjunzuo@163.com
Corresponding author: Jing-He Tan. College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an City, Shandong 271018, China. Fax: +86 0538 8241419. E-mail: tanjh@sdau.edu.cn. Jun-Zuo Wang. Department of Reproduction and Genetics, Tai’an City Central Hospital, Tai’an, Shandong Province, China. E-mail: wjunzuo@163.com
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Summary

Although ethanol treatment is widely used to activate oocytes, the underlying mechanisms are largely unclear. Roles of intracellular calcium stores and extracellular calcium in ethanol-induced activation (EIA) of oocytes remain to be verified, and whether calcium-sensing receptor (CaSR) is involved in EIA is unknown. This study showed that calcium-free ageing (CFA) in vitro significantly decreased intracellular stored calcium (sCa) and CaSR expression, and impaired EIA, spindle/chromosome morphology and developmental potential of mouse oocytes. Although EIA in oocytes with full sCa after ageing with calcium does not require calcium influx, calcium influx is essential for EIA of oocytes with reduced sCa after CFA. Furthermore, the extremely low EIA rate in oocytes with CFA-downregulated CaSR expression and the fact that inhibiting CaSR significantly decreased the EIA of oocytes with a full complement of CaSR suggest that CaSR played a significant role in the EIA of ageing oocytes. In conclusion, CFA impaired EIA and the developmental potential of mouse oocytes by decreasing sCa and downregulating CaSR expression. Because mouse oocytes routinely treated for activation (18 h post hCG) are equipped with a full sCa complement and CaSR, the present results suggest that, while calcium influx is not essential, CaSR is required for the EIA of oocytes.

Keywords

Calcium influxCalcium-sensing receptorEthanol-induced activationIntracellular calcium storesOocyte ageing
Type
Research Article
Information
Zygote , Volume 31 , Issue 4 , August 2023 , pp. 393 - 401
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Copyright
© The Author(s), 2023. Published by Cambridge University Press

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