Hostname: page-component-76fb5796d-25wd4 Total loading time: 0 Render date: 2024-04-25T11:16:38.784Z Has data issue: false hasContentIssue false
  • English
  • Français

Development of magnetically active scaffolds as intrinsically-deformable bioreactors

Published online by Cambridge University Press:  27 June 2017

Darina A. Gilroy ,
Chris Hobbs ,
Valeria Nicolosi ,
Conor T. Buckley ,
Fergal J. O'Brien  and
Cathal J. Kearney Open the ORCID record for Cathal J. Kearney [Opens in a new window]
Darina A. Gilroy
Affiliation:
Tissue Engineering Research Group, Department of Anatomy, Royal College of Surgeons in Ireland (RCSI), Dublin 2, Ireland Trinity Centre for Bioengineering, Trinity College Dublin (TCD), Dublin 2, Ireland
Chris Hobbs
Affiliation:
Advanced Materials and Bioengineering Research (AMBER) Centre, RCSI & TCD, Dublin 2, Ireland CRANN, TCD, Dublin, Ireland School of Physics, TCD, Dublin, Ireland
Valeria Nicolosi
Affiliation:
Advanced Materials and Bioengineering Research (AMBER) Centre, RCSI & TCD, Dublin 2, Ireland CRANN, TCD, Dublin, Ireland School of Chemistry, TCD, Dublin, Ireland
Conor T. Buckley
Affiliation:
Trinity Centre for Bioengineering, Trinity College Dublin (TCD), Dublin 2, Ireland Advanced Materials and Bioengineering Research (AMBER) Centre, RCSI & TCD, Dublin 2, Ireland
Fergal J. O'Brien
Affiliation:
Tissue Engineering Research Group, Department of Anatomy, Royal College of Surgeons in Ireland (RCSI), Dublin 2, Ireland Trinity Centre for Bioengineering, Trinity College Dublin (TCD), Dublin 2, Ireland Advanced Materials and Bioengineering Research (AMBER) Centre, RCSI & TCD, Dublin 2, Ireland
Cathal J. Kearney*
Affiliation:
Tissue Engineering Research Group, Department of Anatomy, Royal College of Surgeons in Ireland (RCSI), Dublin 2, Ireland Trinity Centre for Bioengineering, Trinity College Dublin (TCD), Dublin 2, Ireland Advanced Materials and Bioengineering Research (AMBER) Centre, RCSI & TCD, Dublin 2, Ireland
*
Address all correspondence to Cathal J. Kearney at cathalkearney@rcsi.ie
Get access

Abstract

Mesenchymal stem cell behavior can be regulated through mechanical signaling, either by dynamic loading or through biomaterial properties. We developed intrinsically responsive tissue engineering scaffolds that can dynamically load cells. Porous collagen- and alginate-based scaffolds were functionalized with iron oxide to produce magnetically active scaffolds. Reversible deformations in response to magnetic stimulation of up to 50% were recorded by tuning the material properties. Cells could attach to these scaffolds and magnetically induced compressive deformation did not adversely affect viability or cause cell release. This platform should have broad application in the mechanical stimulation of cells for tissue engineering applications.

Type
Biomaterials for 3D Cell Biology Research Letters
Information
MRS Communications , Volume 7 , Issue 3 , September 2017 , pp. 367 - 374
Copyright
Copyright © Materials Research Society 2017 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1.World Health Organisation: Global Health and Ageing (2011). http://www.who.int/ageing/publications/global_health/en/Google Scholar
2.Arthritis Research UK: Osteoarthritis in General Practice (2013). http://www.arthritisresearchuk.org/policy-and-public-affairs/reports-and-resources/reports.aspxGoogle Scholar
3.Jiang, Y., Jahagirdar, B.N., Reinhardt, R.L., Schwartz, R.E., Keene, C.D., Ortiz-Gonzalez, X.R., Reyes, M., Lenvik, T., Lund, T., Blackstad, M., Du, J., Aldrich, S., Lisberg, A., Low, W.C., Largaespada, D.A., and Verfaillie, C.M.: Pluripotency of mesenchymal stem cells derived from adult marrow. Nature 418, 41 (2002).Google Scholar
4.O'Brien, F.J.: Biomaterials & scaffolds for tissue engineering. Mater. Today 14, 88 (2011).Google Scholar
5.Lutolf, M.P. and Hubbell, J.A.: Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering. Nat. Biotechnol. 23, 47 (2005).Google Scholar
6.Plunkett, N. and O'Brien, F.J.: Bioreactors in tissue engineering. Technol. Health Care. 19, 55 (2011).Google Scholar
7.Temenoff, J.S. and Mikos, A.G.: Review: tissue engineering for regeneration of articular cartilage. Biomaterials 21, 431 (2000).Google Scholar
8.Martin, Y. and Vermette, P.: Bioreactors for tissue mass culture: design, characterization, and recent advances. Biomaterials 26, 7481 (2005).Google Scholar
9.Murphy, S.V. and Atala, A.: Organ engineering—combining stem cells, biomaterials, and bioreactors to produce bioengineered organs for transplantation. BioEssays 35, 163 (2013).Google Scholar
10.Hao, J., Zhang, Y., Jing, D., Shen, Y., Tang, G., Huang, S., and Zhao, Z.: Mechanobiology of mesenchymal stem cells: perspective into mechanical induction of MSC fate. Acta Biomater. 20, 1 (2015).Google Scholar
11.Ivanovska, I.L., Shin, J.-W., Swift, J., and Discher, D.E.: Stem cell mechanobiology: diverse lessons from bone marrow. Trends Cell Biol. 25, 523 (2015).Google Scholar
12.Brady, M.A., Vaze, R., Amin, H.D., Overby, D.R., and Ethier, C.R.: The design and development of a high-throughput magneto-mechanostimulation device for cartilage tissue engineering. Tissue Eng C, Methods 20, 149 (2014).Google Scholar
13.Démarteau, O., Wendt, D., Braccini, A., Jakob, M., Schäfer, D., Heberer, M., and Martin, I.: Dynamic compression of cartilage constructs engineered from expanded human articular chondrocytes. Biochem. Biophys. Res. Commun. 310, 580 (2003).Google Scholar
14.Cezar, C.A., Kennedy, S.M., Mehta, M., Weaver, J.C., Gu, L., Vandenburgh, H., and Mooney, D.J.: Biphasic ferrogels for triggered drug and cell delivery. Adv. Healthcare Mat. 3, 1869 (2014).Google Scholar
15.Kearney, C.J. and Mooney, D.J.: Macroscale delivery systems for molecular and cellular payloads. Nat. Mater. 12, 1004 (2013).Google Scholar
16.Zhao, X., Kim, J., Cezar, C.A., Huebsch, N., Lee, K., Bouhadir, K., and Mooney, D.J.: Active scaffolds for on-demand drug and cell delivery. Proc. Nat. Acad. Sci. USA 108, 67 (2011).Google Scholar
17.Cezar, C.A., Roche, E.T., Vandenburgh, H.H., Duda, G.N., Walsh, C.J., and Mooney, D.J.: Biologic-free mechanically induced muscle regeneration. Proc. Nat. Acad. Sci. USA 113, 1534 (2016).Google Scholar
18.O'Brien, F.J., Harley, B.A., Yannas, I.V., and Gibson, L.J.: The effect of pore size on cell adhesion in collagen-GAG scaffolds. Biomaterials 26, 433 (2005).Google Scholar
19.Haugh, M.G., Jaasma, M.J., and O'Brien, F.J.: The effect of dehydrothermal treatment on the mechanical and structural properties of collagen-GAG scaffolds. J. Biomed. Mater. Res. A 89A, 363 (2009).Google Scholar
20.Augst, A.D., Kong, H.J., and Mooney, D.J.: Alginate hydrogels as biomaterials. Macromol. Biosc. 6, 623 (2006).Google Scholar
21.Lee, K.Y. and Mooney, D.J.: Alginate: properties and biomedical applications. Progr. Polym. Sci. 37, 106 (2012).Google Scholar
22.Gibson, L.J. and Ashby, M.F.: Cellular Solids (Cambridge University Press, Cambridge, UK, 1999).Google Scholar
23.Harley, B.A., Leung, J.H., Silva, E.C.C.M., and Gibson, L.J.: Mechanical characterization of collagen–glycosaminoglycan scaffolds. Acta Biomater. 3, 463 (2007).Google Scholar
24.Delaine-Smith, R.M. and Reilly, G.C.: Mesenchymal stem cell responses to mechanical stimuli. Muscles Ligaments Tendons J. 2, 169 (2012).Google Scholar
25.Schulz, R.M. and Bader, A.: Cartilage tissue engineering and bioreactor systems for the cultivation and stimulation of chondrocytes. Euro. Biophys. J. 36, 539 (2007).Google Scholar
Supplementary material: PDF

Gilroy supplementary material

Gilroy supplementary material

Download Gilroy supplementary material(PDF)
PDF 2 MB