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Aryloxyalkanoate Dioxygenase-12 Soybean Protein Expression

Published online by Cambridge University Press:  20 January 2017

Andrew P. Robinson ,
D. M. Simpson ,
Kerrm Yau ,
Sarah Canada  and
William G. Johnson
Andrew P. Robinson
Affiliation:
Department of Botany and Plant Pathology, 915 W. State Street, Purdue University, West Lafayette, IN 47907
D. M. Simpson
Affiliation:
Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN 46268
Kerrm Yau
Affiliation:
Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN 46268
Sarah Canada
Affiliation:
Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN 46268
William G. Johnson*
Affiliation:
Department of Botany and Plant Pathology, 915 W. State Street, Purdue University, West Lafayette, IN 47907
*
Corresponding author's E-mail: wgj@purdue.edu
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Abstract

New trait technology incorporating 2,4-D resistance in soybean is dependent upon the ability of the plant to metabolize 2,4-D by the aryloxyalkanoate dioxygenase-12 protein (AAD-12). Our objectives were to determine AAD-12 expression during the daytime, throughout the leaf canopy, and before and after 2,4-D treatment for the events DAS-68416-4 and DAS-21606-3. Field experiments were conducted near Wanatah, IN in 2009 and Fowler, IN in 2009, 2010, and 2011. During the daytime, total AAD-12 expression was lowest between 12:30 and 15:30, averaging 161 ng cm−2, as compared to an average of 245 ng cm−2 in the morning and 243 ng cm−2 in the evening. The youngest fully emerged trifoliate in the DAS-68416-4 event had the highest AAD-12 expression, with means ranging from 369 to 390 ng cm−2, while the older leaves maintained a lower level of expression, 171 to 211 ng cm−2. The youngest leaves of event DAS-21606-3 had the highest level of AAD-12 expression (205 to 225 ng cm−2), while the level of AAD-12 was lower in older leaves (71 to 149 ng cm−2). In general, 2,4-D treatments did not reduce AAD-12 expression at 3, 7, 14, and 21 days after treatment; however, in a few instances AAD-12 expression was increased or decreased by 8 to 11% after 2,4-D treatment. Expression of AAD-12 was between 152 to 390 ng cm−2 for DAS-68416-4 and from 71 to 244 ng cm−2 for DAS-21606-3.

Keywords

2,4-DGlycine max (L.) Merr., soybean.2,4-dichlorophenoxyacetic acid2,4-D-tolerant soybeantransgenic soybean
Type
Physiology/Chemistry/Biochemistry
Information
Weed Science , Volume 63 , Issue 1 , March 2015 , pp. 229 - 234
Copyright
Copyright © Weed Science Society of America 

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