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Treatment of chronic mastoiditis by grafting of mastoid cavities with autologous epithelial layers generated by in vitro culture of buccal epithelium

Published online by Cambridge University Press:  29 June 2007

D. J. Premachandra ,
B. Woodward ,
C. M. Milton ,
R. J. Sergeant  and
J. W. Fabre
D. J. Premachandra*
Affiliation:
Department of ENT Surgery, Kent and Sussex Hospital, Tunbridge Wells, Kent TN4 8AT.
B. Woodward
Affiliation:
Regional Plastic Surgical Unit, Queen Victoria Hospital, East Grinstead, Sussex RH19 3DZ.
C. M. Milton
Affiliation:
Department of ENT Surgery, Kent and Sussex Hospital, Tunbridge Wells, Kent TN4 8AT.
R. J. Sergeant
Affiliation:
Department of ENT Surgery, Kent and Sussex Hospital, Tunbridge Wells, Kent TN4 8AT.
J. W. Fabre
Affiliation:
Blond Mclndoe Centre for Medical Research, Queen Victoria Hospital, East Grinstead, Sussex RH19 3DZ.
*
Mr D. J. Premachandra, Consultant E.N.T. Surgeon, James Paget Hospital, Great Yarmouth, Norfolk NR31 6LA.
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Abstract

Autologous cultured epithelial layers were established from biopsies from the mucosa of the cheek, a nonkeratinizing region of the oral cavity. These were grafted to the unepithelialized mastoid cavities of nine patients with chronic mastoiditis and severe otorrhoea varying from two to 30 years' duration. All procedures were performed on an out-patient basis, with no anaesthesia except for topical anaesthesia for the mucosal biopsy. In seven of the patients the grafts took well, with complete resolution of the otorrhoea for a minimum follow-up period of eight months. In one patient there was a partial take of the graft with substantial improvement in the rate of discharge. The mastoid cavities of two patients were biopsied five months after grafting, and demonstrated a stratified squamous epithelium, with keratinization of the epithelium clearly evident.

Keywords

Mastoid surgeryMonta mucosa
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 105 , Issue 6 , June 1991 , pp. 413 - 416
Copyright
Copyright © JLO (1984) Limited 1991

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References

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