Hostname: page-component-8448b6f56d-jr42d Total loading time: 0 Render date: 2024-04-19T07:09:01.241Z Has data issue: false hasContentIssue false
  • English
  • Français

Extraction of Three-Dimensional Information of Biological Membranous Tissue with Scanning Confocal Infrared Laser Microscope Tomography

Published online by Cambridge University Press:  06 August 2013

Soonwook Kwon ,
Se-Bum Choi ,
Min Gyu Park ,
Hyunung Yu ,
Seung-Woo Suh  and
Im Joo Rhyu
Soonwook Kwon
Affiliation:
Department of Anatomy, Korea University College of Medicine, Seoul 136-705, Korea
Se-Bum Choi
Affiliation:
Korea Research Institute of Standards and Science, Daejon 305-340, Korea
Min Gyu Park
Affiliation:
Korea Research Institute of Standards and Science, Daejon 305-340, Korea
Hyunung Yu*
Affiliation:
Korea Research Institute of Standards and Science, Daejon 305-340, Korea
Seung-Woo Suh
Affiliation:
Department of Orthopedics, Korea University Guro Hospital, Seoul 152-703, Korea
Im Joo Rhyu*
Affiliation:
Department of Anatomy, Korea University College of Medicine, Seoul 136-705, Korea
*
†Corresponding author. E-mail: peacewithu@kriss.re.kr
‡Corresponding author. E-mail: irhyu@korea.ac.kr
Get access

Abstract

The “LEXT” confocal laser scanning microscope has been used for the three-dimensional (3D) imaging of the surface of specimens, especially in materials science fields, by the penetration of near-infrared (NIR) light without mechanical cutting, deposition, or other specimen pretreatment. Noninvasive investigation of various biological tissues such as human spinal dura mater, rat aorta, and cornea without the dehydration process was successfully carried out with the “LEXT,” in order to access both surface and internal topographic images of the biological structures at a good status of the wet tissue such as in vivo, especially in measuring tissue thickness. The confocal NIR laser microscopy offers the viable means to visualize tissue architecture and its thickness in microdomain to integrate 3D images efficiently. We believe that the “LEXT” has a good application for biological researchers to study biomaterials, and it would be useful as a diagnostic tool in the near future.

Keywords

laser microscopeimagingconfocalinfraredscanningdura matermembrane
Type
Research Article
Information
Microscopy and Microanalysis , Volume 19 , Supplement S5: IUMAS , August 2013 , pp. 194 - 197
Copyright
Copyright © Microscopy Society of America 2013 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Clay, R.S. & Court, T.H. (1975). The History of the Microscope: Compiled from Original Instruments and Documents, up to the Introduction of the Achromatic Microscope. London: Holland Press.Google Scholar
Dubey, G.P. & Ben-Yehuda, S. (2011). Intercellular nanotubes mediate bacterial communication. Cell 144(4), 590–600.10.1016/j.cell.2011.01.015Google Scholar
Heinrichs, A. (2009). Stains and fluorescent dyes. Nat Cell Biol 11, S7.10.1038/ncb1939Google Scholar
Jalbert, I., Stapleton, F., Papas, E., Sweeney, D.F. & Coroneo, M. (2003). In vivo confocal microscopy of the human cornea. Br J Ophthalmol 87(2), 225–236.10.1136/bjo.87.2.225Google Scholar
Kuypers, L.C., Decraemer, W.F. & Dirckx, J.J. (2006). Thickness distribution of fresh and preserved human eardrums measured with confocal microscopy. Otol Neurotol 27(2), 256–264.10.1097/01.mao.0000187044.73791.92Google Scholar
Mahapatro, A., Lee, G., Jeong, K. & Janes, D. (2009). Stable and reproducible electronic conduction through DNA molecular junctions. Appl Phys Lett 95, 083106-3.10.1063/1.3186056Google Scholar
McEwen, B.F. & Marko, M. (1999). Three-dimensional transmission electron microscopy and its application to mitosis research. Methods Cell Biol 61, 81–111.10.1016/S0091-679X(08)61976-7Google Scholar
Ruska, E. (1987). Nobel lecture. The development of the electron microscope and of electron microscopy. Biosci Rep 7, 607–629.10.1007/BF01127674Google Scholar
Schulz, D., Iliev, M.E., Frueh, B.E. & Goldblum, D. (2003). In vivo pachymetry in normal eyes of rats, mice and rabbits with the optical low coherence reflectometer. Vision Res 43, 723–728.10.1016/S0042-6989(03)00005-1Google Scholar
Sun, C.K., Chen, C.C., Chu, S.W., Tsai, T.H., Chen, Y.C. & Lin, B.L. (2003). Multiharmonic-generation biopsy of skin. Opt Lett 28(24), 2488–2490.10.1364/OL.28.002488Google Scholar