The major facilitator superfamily domain 2a protein was identified recently as a lysophosphatidylcholine (LPC) symporter with high affinity for LPC species enriched with DHA (LPC-DHA). To test the hypothesis that reproductive state and choline intake influence plasma LPC-DHA, we performed a post-hoc analysis of samples available through 10 weeks of a previously conducted feeding study, which provided two doses of choline (480 and 930 mg/d) to non-pregnant (n=21), third-trimester pregnant (n=26), and lactating (n=24) women; all participants consumed 200 mg of supplemental DHA and 22% of their daily choline intake as deuterium-labeled choline. The effects of reproductive state and choline intake on total LPC-DHA (expressed as a percentage of LPC) and plasma enrichments of labeled LPC and LPC-DHA were assessed using mixed and generalized linear models. Reproductive state interacted with time (p=0.001) to influence total LPC-DHA, which significantly increased by week 10 in non-pregnant women, but not in pregnant or lactating women. Contrary to total LPC-DHA, patterns of labeled LPC-DHA enrichments were discordant between pregnant and lactating women (p<0.05), suggestive of unique, reproductive state-specific mechanisms that result in the reduced production and/or enhanced clearance of LPC-DHA during pregnancy and lactation. Regardless of reproductive state, women consuming 930 versus 480 mg choline/d exhibited no change in total LPC-DHA but higher d3-LPC-DHA (p=0.02), indicating that higher choline intakes favor production of LPC-DHA from the PEMT pathway of phosphatidylcholine biosynthesis. Our results warrant further investigation into the effect of reproductive state and dietary choline on LPC-DHA dynamics and its contribution to DHA status.