Our goal was to assess the knowledge, attitudes, and practices regarding infection control and standard precautions among a group of nursing and midwifery instructors and students in Iran. A survey questionnaire was completed by 273 nursing and midwifery instructors and students at Shiraz University Medical Sciences during the period from May to November 2002. Two hundred thirty-one (90.9%) of the participants reported that they needed additional infection control education, especially on standard isolation precautions. There was a linear positive correlation between knowledge, practice, and attitude scores for the group of nursing, auxiliary nursing, and midwifery instructors, as well as their students (P < .05). Our study shows that there is an urgent need for evaluating education on infection control practices and standard precautions in general, as well as for structured infection control programs among nursing and midwifery staff.