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Annexin A2 (ANXA2) is reported to be associated with cancer development. To investigate the roles ANXA2 plays during the development of cancer, the RNAi method was used to inhibit the ANXA2 expression in caco2 (human colorectal cancer cell line) and SMMC7721 (human hepatocarcinoma cell line) cells. The results showed that when the expression of ANXA2 was efficiently inhibited, the growth and motility of both cell lines were significantly decreased, and the development of the motility relevant microstructures, such as pseudopodia, filopodia, and the polymerization of microfilaments and microtubules were obviously inhibited. The cancer cell apoptosis was enhanced without obvious significance. The possible regulating pathway in the process was also predicted and discussed. Our results suggested that ANXA2 plays important roles in maintaining the malignancy of colorectal and hepatic cancer by enhancing the cell proliferation, motility, and development of the motility associated microstructures of cancer cells based on a possible complicated signal pathway.
Compared with end pumping fiber combiner, one of the advantages for side pumping combiner is the unlimited pumping points, which means multi-point or cascaded side pumping can be realized. However, the loss mechanism of the cascaded structure is rarely discussed. In this paper, we present the numerical and experimental investigation about the loss mechanism of a two-stage-cascaded side pumping combiner based on tapered-fused technique. The influence of loss mechanism on the coupling efficiency and thermal load of the fiber coating is analyzed according to simulations and experiments with different tapering ratios for the first stage. Based on the analysis, a cascaded component with total pump coupling efficiency of 96.4% handling a pump power of 1088 W is achieved by employing 1018 nm fiber laser as the pump source. Future work to further improve the performance of a cascaded side pumping combiner is discussed and prospected.
This paper investigates a wideband and low axial ratio circularly polarized (CP) antenna, which is composed of a monopole on a novel polarization rotating reflective surface (PRRS) based on a corner-truncated artificial magnetic conductor (AMC) structure. By adjusting the dimensions of truncated corner properly, the PRRS has two polarization rotation (PR) frequency points. Then, a large PR band of 18% (5.55–6.65 GHz) can be achieved with two adjacent PR frequency points coming together. The profile of the newly PRRS is only0.04λ0. With corner-truncated AMC-based PRRS loading, a measured impedance bandwidth of 1.8 GHz (5.4–7.2 GHz) and the 3 dB axial ratio bandwidth of 1 GHz (5.55–6.65 GHz) could be obtained by the monopole antenna and validated by measurements. The values of AR were well below 1 dB at most of the CP region, which show a perfect CP performance. Moreover, the proposed antenna has exhibited a large axial ratio beamwidth in both the xoz- and yoz-planes and a peak gain of 6.1 dBic within the operational bandwidth.
An assessment of the glaciological and meteorological characteristics of Dome A, the summit of the East Antarctic ice sheet, is made based on field investigations during the austral summer of 2004/05. Knowledge of these characteristics is critical for future international studies such as deep ice-core drilling. The assessment shows that: (1) Dome A is characterized by a very low 10m depth firn temperature, –58.3˚C (nearly 3˚C lower than at EPICA Dome C and 1˚C lower than at Vostok). (2) Automatic weather station (AWS) measurements of snow surface height and reference layers in a snow pit indicate the present-day snow accumulation rate at Dome A is within the range 1–3cmw.e. a–1. Densification models suggest a range of 1–2cmw.e. a–1. This is lower than at other sites along the ice divide of East Antarctica (IDEA). Annual layers at Dome A are thus potentially thinner than at other sites, so that a longer record is preserved in a given ice thickness. (3) The average wind speed observed at Dome A (<4ms–1) is lower than at other sites along IDEA. Together, these parameters, combined with radio-echo sounding data and information on the subglacial drainage distribution beneath Dome A, suggest Dome A as a candidate site for obtaining the oldest ice core.
Stakes at 2 km intervals were installed in January 1997 and remeasured in February 1998, January 1999, January 2005 and during the 2007/08 austral summer along a 1248 km traverse route from Zhongshan station to Dome A, East Antarctica. Based on topographical parameters, meteorological features and the records of ∼650 stakes and six stake arrays, the route is divided into five zones. We find that the snow accumulation rate decreases with increasing altitude as one progresses inland, except in the zone 800–1128 km from the coast, where the average annual accumulation rate is higher than in the zone 524–800 km from the coast. The Dome A zone (1128–1248 km) has the lowest accumulation rate (35 kg m−2 a−1, 2005–08) due to having the highest elevation and being furthest from the coast. The surface mass balance in the region 202–1128 km from the coast exhibits no temporal change from 1999–2005 to 2005–08, but there is a change in the accumulation distribution. The zone from 202 to 524 km shows a decrease in surface mass balance from 84 kg m−2 a−1 in 1999–2005 to 67 kg m−2 a−1 in 2005–08, while the zone between 800 and 1128 km shows an increase from 67 kg m−2 a−1 in 1999–2005 to 75 kg m−2 a−1 in 2005–08.
Stable-oxygen-isotope ratios (δ18O) of precipitation and ice-core samples collected from the headwaters of the Urümqi river, Tien Shan, China, were used to test the relationship between δ18O and contemporaneous surface air temperature (Ta). A strong temporal relationship is found between δ18O in precipitation and Ta, particularly for the monthly averages which remove synoptic-scale influences such as changes in condensation level, condensation temperature and moisture sources ( Yao and others, 1996). Linear fits as high as 0.95‰° C-1 for precipitation events and 1.23‰° C-1 for monthly averages are found. Although the δ18O amplitude in ice cores drilled at the nearby Ürümqi glacier No.1(~2 km from the precipitation sampling site) decreased dramatically compared to the precipitation samples, the ice-core records of annually averaged δ18 are still positively correlated with contemporaneous air temperature, especially summer air temperature, at the nearby Daxigou meteorological station. Nevertheless, the relationship between the ice-core δ18O records and contemporaneous air temperature is less significant than that for the precipitation samples due to depositional and post-depositional modification processes, which are highlighted by the successive snow-pit δ18O profiles from the Ürümqi glacier No.1. Our results might extend the application of high-altitude and subtropical ice-core δ18O —Ta records for paleoclimate reconstruction.
In this paper, we focus on studying the high energy emission of GRB 160625B. The lightcurve of prompt emission is composed of three episodes: short-soft precursor, hard main burst, and possible long extended emission. The spectra of first and third episode can be fitted by a multi-color blackbody and cutoff power-law model, respectively. However, the spectrum of second episode was contributed by both multi-color blackbody and cutoff power-law. One can estimate the Lorenz factor of jet of first two episodes by invoking photosphere model as Γ0 ~ 175 and 1694, respectively. It suggests that the ejecta of this case evolved from photosphere dominated initially to internal shock later. On the other hand, the optical emission is very bright during the second episode, which is likely a prompt optical emission. Finally, a more shallower normal decay segment appeared, which is consistent with standard external shock model.
We present calculation of critical voltage for AlGaN/GaN high electron mobility transistors (HEMTs) with GaN cap layer. The calculation includes mechanical stress and relaxable energy in the GaN/AlGaN barrier layer. Under high voltage conditions, the high electric field results in an increase in stored relaxable energy. If this exceeds a critical value, crystallographic defects are formed. This degradation mechanism is voltage driven and characterized by a critical voltage beyond which non-reversible degradation takes place. The dependence of the GaN cap layer’s thickness on the critical voltage has been discussed. The calculated results indicate that thicker GaN cap layer results in higher critical voltage.
A theoretical study of transconductance characteristics (gm − Vgs profile) of AlGaN/GaN high electron mobility transistors (HEMTs) with a graded AlGaN layer is given in this paper. The calculations were made using a self-consistent solution of the Schrödinger-Poisson equations and an AlGaN/GaN HEMTs numerical device model. Transconductance characteristics of the devices are discussed while the thickness and Al composition of the graded AlGaN layer are optimized. It is found that graded AlGaN layer structure can tailor device’s gm − Vgs profile by improving polar optical phonon mobility and interface roughness mobility. Good agreement is obtained between the theoretical calculations and experimental measurements over the full range of applied gate bias.
The AlGaN/GaN/InGaN/GaN double heterojunction high electron mobility transistors (DH-HEMTs) sample has been grown by MOCVD on (0 0 0 1) sapphire substrate. The structure features a 7 nm In0.046Ga0.954N interlayer determined by Rutherford backscattering (RBS). Since the polarization field in the InGaN interlayer is opposite to it in the AlGaN layer, an additional potential barrier is introduced between the two-dimensional electron gas (2DEG) channel and buffer, leading to enhanced carrier confinement and improved buffer isolation. The GaN layers between the AlGaN layer and InGaN interlayer are divided into two layers consisting of GaN channel layer which provides high mobility 2DEG grown at 1070 °C and GaN spacer layer grown at the same temperature as InGaN interlayer (800 °C) to prevent indium diffusion. RBS measurement confirms that the 3 nm GaN spacer layer isolates the InGaN interlayer well and free from diffusion. Hall measurement has been performed, the mobility as high as 1552 cm2/V s at room temperature is obtained and the sheet carrier density is 1.55 × 1013 cm−2. The average sheet resistance is 331 Ω/sq, respectively. The mobility obtained in this paper is about 20% higher than similar structures reported.
We report the fabrication of AlGaN/GaN high electron mobility transistors (MIS-HEMTs) with a high breakdown voltage by employing a metal-insulator-semiconductor (MIS) gate structure using Si3N4 insulator. The Si3N4 films were deposited by plasma enhanced chemical vapor deposition (PECVD) as the surface passivation, interlayer films and the gate dielectric. In comparison with Schottky-gate HEMTs, the gate leakage currents of MIS-HEMTs exhibited three orders of magnitude reduction. With similar device structures, the off-state breakdown voltage of MIS-HEMTs was 1050 V with a specific on-resistance of 4.0 mΩ cm2, whereas the breakdown voltage and specific on-resistance of SG-HEMTs were 740 V and 4.4 mΩ cm2, respectively. In addition, the MIS-HEMTs exhibited little current slump in the pulsed measurements and possessed faster switch speed than Si MOSFET. We demonstrate that AlGaN/GaN MIS-HEMTs are promising not only for microwave applications but also for high power switching applications.
Spermatogonial stem cells (SSCs) have the ability to self-renew and offer a pathway for genetic engineering of the male germ line. Cryopreservation of SSCs has potential value for the treatment of male infertility, spermatogonial transplantation, and so on. In order to investigate the cryopreservation effects of different cryoprotectants on murine SSCs, 0.2 M of low-density lipoproteins (LDL), trehalose and soybean lecithin were added to the cryoprotective medium, respectively, and the murine SSCs were frozen at −80°C or −196°C. The results indicated that the optimal recovery rates of murine SSCs in the cryoprotective medium supplemented with LDL, trehalose and soybean lecithin were 92.53, 76.35 and 75.48% at −80°C, respectively. Compared with freezing at −196°C, the optimum temperature for improvement of recovery rates of frozen murine SSCs, cryopreservation in three different cryoprotectants at −80°C, were 17.11, 6.68 and 10.44% respectively. The recovery rates of murine SSCs in the cryoprotective medium supplemented with 0.2 M LDL were significantly higher than that of other cryoprotectants (P < 0.05). Moreover, the recovery rates were demonstrated to be greater at −80°C compared with at −196°C (P < 0.05). In conclusion, 0.2 M of LDL could significantly protect murine SSCs at −80°C. In the freezing–thawing process, LDL is responsible for the cryopreservation of murine SSCs because it can form a protective film at the surface of membranes. However, more research is needed to evaluate and understand the precise role of LDL during the freezing–thawing of SSCs.
Understanding the factors that affect the nesting success of threatened birds is essential in designing effective conservation strategies. Here we compare nesting success of the Reed Parrotbill Paradoxornis heudei in annually harvested and non-harvested reedbed habitats in the Chongming Dongtan Nature Reserve in China, by recording the number of nestlings produced per nest and the causes of nest failure. We modelled daily nest survival rate (DSR) by considering the effects of harvesting, vegetation characteristics, year, date, advancement within the breeding season, nest age and nearest-nest distance, using the program MARK. Nest densities, but not the number of fledglings per nest, were significantly lower in harvested than non-harvested reedbed habitats. The best-fit DSR model estimated constant survival; none of the tested co-variables had significant effects. Moreover, harvesting did not affect the date of breeding initiation, likelihood of nest failure, or causes of nest failure, as vegetation cover was not significantly different between harvested and non-harvested reedbeds during the whole breeding season. Nest failure following adverse weather conditions was unusually common in harvested and non-harvested reedbeds, accounting for as many nest failures as depredation. However, comparisons with other studies suggest that deriving a conclusion on the impact of harvesting on nesting success is not straightforward and is probably linked to environmental characteristics affecting reed growth.
Cytosol Ca2+ overload plays a vital role in ischemic neuronal damage, which is largely contributed by the Ca2+ influx through L-type voltage-gated calcium channels (L-VGCCs) and N-methyl-D-aspartate (NMDA) type glutamate receptors. In this article, L-VGCCs were activated by depolarization to investigate the cross-talk between NMDA receptors and L-VGCCs.
Depolarization was induced by 20 minutes incubation of 75 mM KCl in cultured rat cortical neuron. Apoptosis-like neuronal death was detected by DAPI staining. Tyrosine phosphorylation of NMDA receptor subunit 2A (NR2A), interactions of Src and NR2A were detected by immunoblot and immunoprecipitation.
Depolarization induced cortical neuron apoptosis-like cell death after 24 hours of restoration. The apoptosis was partially inhibited by 5 mM EGTA, 100 μM Cd2+, 10 μM nimodipine, 100 μM genistein, 20 μM MK-801, 2 μM PP2 and combined treatment of nimodipine and MK-801. NR2A tyrosine phosphorylation increased after depolarization, and the increase was inhibited by the drugs listed above. Moreover, non-receptor tyrosine kinase Src bound with NR2A after depolarization and restoration. The binding was also inhibited by the drugs listed above.
The results indicated that depolarization-induced neuronal death might be due to extracellular Ca2+ influx through L-VGCCs and subsequently Src activationmediated NR2A tyrosine phosphorylation.
The I-V characteristics of AlGaN/GaN high electron mobility transistors in the temperature range between 100 K and 300 K are studied. It is found that both the maximum drain-source current and transconductance decrease with the increase of temperature. Decrease of the electron mobility with increasing temperature is considered to be the main cause for that condition. The threshold voltage shows a forward shift, which can be explained by the increase of Schottky barrier with increasing temperature. It is found that at VGS = 0 V the drain-source current reduces with the ascending temperature, which should be due to the variation of the electron mobility with the temperature. While at VGS = −5 V the drain-source current is found to increase with the ascending temperature, it is suggested to be caused by the positive temperature coefficient of the electron transport in the depleted region.
The temperature dependence of the I-V characteristics on Au/Ni-HEMT Schottky contacts was measured and analyzed. Large deviations from the thermionic emission and thermionic-field emission model were observed in the I-V-T characteristics. The thin surface barrier model only fits the measured curves in the high bias region, but deviates drastically in the low bias region. Using a revised thin surface barrier model, the calculated curves match well with the measured curves. It is also found that tunneling emission model is the dominant current transport mechanism at low temperature, yet thermionic-field emission model is the dominant current transport mechanism at high temperature.
The objective of this study was to test if intracytoplasmic sperm injection (ICSI)-mediated gene transfer was an effective method in the production of transgenic rabbit embryos. Rabbit sperm diluted in different media with various pH were treated by freezing without cryoprotectant, and their ability for DNA uptake was determined. In these experiments using production of transgenic rabbit embryos by ICSI, exogenous genes at three concentrations and of two conformation types were used. The rate of DNA association to the sperm seen by rhodamine-tagged DNA encoding green fluorescent protein (GFP) was 90.0%, 92.7%, 91.0%, 91.7%, and 92.3%, respectively in TCM199, DM, DPBS, CZB, and HCZB media. The DNA attachment to sperm was not affected by media pH within the range of 5.4–9.4 (p > 0.05). Expression of GFP first occurred at the 2-cell stage and continued to blastocyst formation. DNA concentration (between 5, 10, and 20 ng/μl) or conformation (linear and circular) had no effect on the production rate of transgenic embryos. These results indicated that genetically modified rabbit blastocysts can be efficiently produced by ICSI technique.
The Reed Parrotbill Paradoxornis heudei is an endemic reedbed-inhabiting passerine of east Asia. In the Shanghai municipality, which harbours significant populations of this species, almost all reedbed surfaces are annually harvested. Furthermore, the reedbeds are being invaded by Smooth Cordgrass Spartina alterniflora, an introduced species that can outcompete the native Common Reed Phragmites australis. In this paper, we have shown that Reed Parrotbills do not nest in areas dominated by Smooth Cordgrass and avoid using them. In the areas that are primarily composed of Common Reed, the densities of birds are higher in the unharvested sections. The birds appear to select nesting sites with low Smooth Cordgrass densities, tall reed stems, and relatively equal densities of both dry and green stems. Reed harvesting activity results in vegetation that is too low for bird nesting. However, no nests were found in areas where the reeds had not been harvested for several years and had high densities of dry reed stems; these results could be attributed to the fact that the high density of broken stems reduced the vegetation cover. On the basis of our results, we recommend implementation of four years harvesting-cycle rotation and avoidance of reclamation in reedbeds which have not been invaded by Smooth Cordgrass.
Twins could play a crucial role in our understanding of genetic contributions to numerous etiologically complex disorders. In China, although adult twins are relatively rare, twins will become increasingly available due to increasing twin birth rates. Thus, child twin data will be a valuable resource to contribute to the field of child and adolescent psychopathology. The first twin database of children aged from 6 to 16 was established in Chongqing, R.P., China. In this article, we will discuss our experiences in establishing the twin database, completed in three steps — the first step being to search and identify twins, the second being to keep contact with the twins and the final being to seek cooperation with the twin families, and its future prospects. Our twin database has proven to be an efficient method for the investigation and data collection of twin children in China. The results of our present study suggest that the inclusion of twin information in the residence registration of the public security bureaus in the future may ensure a smooth run of research based on the demographic resources. We propose that school networks may be adopted as the preferred method of collection of twin records for future studies.