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Identifying the relative importance of urban and non-urban land-use types for potential denitrification derived N2O at a regional scale is critical for quantifying the impacts of human activities on nitrous oxide (N2O) emission under changing environments. In this study we used a regional dataset from China including 197 soil samples and six land-use types to evaluate the main predictors (land use, heavy metals, soil pH, soil moisture, substrate availability, functional and broad microbial abundances) of potential denitrification using multivariate and pathway analysis. Our results provide empirical evidence that soils on farms have the greatest potential denitrifying ability (PDA) (10.92±6.08ng N2O-N·g–1 dry soil·min–1) followed by urban soil (6.80±5.35ng N2O-N·g–1 dry soil·min–1). Our models indicate that land use (low vs. high human activity), followed by total nitrogen (TN) and heavy metals (Cu, Zn, Pb, Cd) was the most important driver of PDA. In addition, our path analysis suggests that at least part of the impacts of land use on potential denitrification were mediated via microbial abundance, soil pH and substrates including TN, dissolved organic carbon and nitrate. This study identifies the main predictors of denitrification at a regional scale which is needed to quantify the impact of human activities on ecosystem functionality under changing conditions.
Mach reflection in steady supersonic flow with two incident shock waves is studied. The second incident shock wave is produced by an additional deflection of the wedge lower surface, at some point ensuring that the two incident shock waves would intersect at the reflecting surface in case of normal reflection. Both theory and computational fluid dynamics (CFD) are used to study the flow structure and the influence of the second incident shock wave. The overall flow configuration, in case of Mach reflection, is shown to be composed of a triple shock structure, a shock/shock interaction structure and a shock/slipline reflection structure. Similar phenomenon, triggered by a high downstream pressure, has been observed before numerically, but not studied theoretically. The second incident shock wave reflects over the slipline to deflect the slipline more towards the reflecting surface, increasing thus the Mach stem height, advancing the transition of regular reflection to Mach reflection of the first incident shock wave, and causing an inverted Mach reflection below the usual von Neumann condition. A Mach stem height model built for a weak second incident shock wave is used to study the influence of the second incident shock wave on the Mach stem height. Both theory and CFD predict a maximum of the Mach stem height at some additional wedge deflection angle.
In this paper, we introduce a lower extremity exoskeleton CUHK-EXO that is developed to help paraplegic patients, who have lost the motor and sensory functions of their lower limbs to perform basic daily life motions. Since the sit-to-stand and stand-to-sit (STS) motion is the first step for paraplegic patients toward walking, analysis of the exoskeleton's applicability to the STS motion assistance is performed. First, the human-exoskeleton system (HES) is modeled as a five-link model during the STS motion, and the center of pressure (COP) on the ground and center of gravity of the whole system are calculated. Then, a description of the CUHK-EXO hardware design is presented, including the mechatronics design and actuator selection. The COP position is an important factor indicating system balance and wearer's comfort. Based on the COP position, a trajectory online modification algorithm (TOMA) is proposed for CUHK-EXO to counteract disturbances, stabilize system balance, and improve the wearer's comfort in the STS motion. The results of STS motion tests conducted with a paraplegic patient demonstrate that CUHK-EXO can provide a normal reference pattern and proper assistive torque to support the patient's STS motion. In addition, a pilot study is conducted with a healthy subject to verify the effectiveness of the proposed TOMA under external disturbances before future clinical trials. The testing results verify that CUHK-EXO can counteract disturbances, and help the wearer perform the STS motion safely and comfortably.
Disclosing the diagnosis of Alzheimer's disease (AD) to a patient is controversial. There is significant stigma associated with a diagnosis of AD or dementia in China, but the attitude of the society toward disclosure of such a diagnosis had not been formally evaluated prior to our study. Therefore, we aimed to evaluate the attitude toward disclosing an AD diagnosis to patients in China with cognitive impairment from their caregivers, and the factors that may affect their attitude.
We designed a 17-item questionnaire and administered this questionnaire to caregivers, who accompanied patients with cognitive impairment or dementia in three major hospitals in Shanghai, China. The caregiver's attitude toward disclosing the diagnosis of AD as evaluated by the questionnaire was compared to that of disclosing the diagnosis of terminal cancer.
A majority (95.7%) of the 175 interviewed participants (mean 14.2 years of education received) wished to know their own diagnosis if they were diagnosed with AD, and 97.6% preferred the doctor to tell their family members if they were diagnosed with AD. If a family member of the participants suffered from AD, 82.9% preferred to have the diagnosis disclosed to the patient. “Cognitive impairment” was the most accepted term by caregivers to disclose AD diagnosis in Chinese.
This study suggests most of the well-educated individuals in a Chinese urban area favored disclosing the diagnosis when they or their family members were diagnosed with AD.
In this paper, a tri-band bandpass filter (BPF) using asymmetric stub-loaded stepped-impedance resonator (SL-SIR) is presented. The asymmetric characteristic of SL-SIR broadens degrees of freedom for three controllable modes design. Also, the coupling coefficients (Mij) and the external quality factors (Qei) at each passband of the filter can be independently adjusted by the proposed mixed-type feedline structure. Besides, multi-transmission zeros are produced to improve the isolation and selectivity of the passbands. Finally, a tri-band BPF is operated at 1.9 GHz (time division long term evolution – TD-LTE band), 3.2 GHz (worldwide interoperability for microwave access – WiMAX band), and 5.8 GHz (wireless local area networks – WLAN band) and their insertion loss are 1.03, 0.94, and 1.27 dB, respectively. The measured results of the fabricated tri-band BPF exhibit good agreement with simulated results.
As a well-known phosphatized Lagerstätte, the Ediacaran Weng'an biota in central Guizhou Province of South China contains diverse acanthomorphic acritarchs, algal thalli, tubular microfossils as well as various spheroidal fossils. These fossils provide crucial palaeontological evidence for the radiation of multicellular eukaryotes after the termination of the Neoproterozoic global glaciation. While the Weng'an biota is generally considered as early Ediacaran in age on the basis of phosphorite Pb–Pb isochron ages ranging from 572 Ma to 599 Ma, the reliability and accuracy of these age data have been questioned and some geologists have proposed that the Weng'an biota may be younger than 580 Ma instead. Here we report a SIMS zircon U–Pb age of 609 ± 5 Ma for a tuffaceous bed immediately above the upper phosphorite unit in the Doushantuo Formation at Zhangcunping, Yichang, South China. Litho-, bio- and chemostratigraphic correlations suggest that the upper phosphorite unit at Zhangcunping can be well correlated with the upper phosphorite unit at Weng'an, which is the main horizon of the Weng'an biota. We therefore conclude that the Weng'an biota could be as old as 609 ± 5 Ma.
Seven-band grouper (Hyporthodus septemfasciatus) is a commercial rocky reef fish in East Asia that has been regarded as a promising species for aquaculture. To investigate the broodstock contributions to offspring for the sustainability of fry production, 62 individuals of H. septemfasciatus from two broodstocks and one offspring population were analysed using fluorescent-AFLP. A total of 602 bands were amplified and 70.10% of them were polymorphic. The numbers of polymorphic loci were 308 (Pbroodstock I = 55.50%) and 356 (Pbroodstock II = 63.12%) in the two broodstocks, and 294 (Poffspring = 52.88%) in the offspring, respectively. The average values of Shannon diversity index (I) and expected heterozygosity (H) were higher in the broodstock (Ibroodstock I = 0.281, Ibroodstock II = 0.244, Hbroodstock I = 0.185, Hbroodstock II = 0.161) than those in the offspring (Ioffspring = 0.243, Hoffspring = 0.161). AMOVA and FST analyses showed that significant genetic differentiation between broodstock and offspring populations, and limited effective broodstock population size has contributed to the offspring. Both STRUCTURE and Principal Coordinates Analysis (PCoA) also showed the three populations composed of two stocks and most offspring individuals (95.0%) only originated from 44.0% of the individuals of broodstock I, which may have negative effects on sustainable fry production. Therefore, genetic variation between broodstock and offspring should be monitored, and large effective size of broodstock should be employed to ensure the success of commercial breeding programmes. Our data provide a useful genetic basis for future planning of sustainable culture and management of H. septemfasciatus.
The mutualism between fig trees and their wasp pollinators is a model system for many ecological and evolutionary studies. However, the immature stages of pollinating fig wasps have rarely been studied. We monitored developing fig wasps of known ages and performed a series of dissections at 24 h intervals to identify key developmental traits of Ceratosolen solmsi marchali Mayr (Hymenoptera: Agaonidae), a pollinator of Ficus hispida L. (Moraceae). We identified where in the Ficus ovary eggs were deposited and time to hatch. We were also able to identify the timing and key underlying characters of five larval instars, three sub-pupal stages, and a single prepupal stage. We provide detailed morphological descriptions for the key stages and report some behavioral observations of the wasps in the several developmental stages we recorded. Scanning electron microscope images were taken.
The relationship between recurrent major depression (MD) in women and suicidality is complex. We investigated the extent to which patients who suffered with various forms of suicidal symptomatology can be distinguished from those subjects without such symptoms.
We examined the clinical features of the worst episode in 1970 Han Chinese women with recurrent DSM-IV MD between the ages of 30 and 60 years from across China. Student's t tests, and logistic and multiple logistic regression models were used to determine the association between suicidality and other clinical features of MD.
Suicidal symptomatology is significantly associated with a more severe form of MD, as indexed by both the number of episodes and number of MD symptoms. Patients reporting suicidal thoughts, plans or attempts experienced a significantly greater number of stressful life events. The depressive symptom most strongly associated with lifetime suicide attempt was feelings of worthlessness (odds ratio 4.25, 95% confidence interval 2.9–6.3). Excessive guilt, diminished concentration and impaired decision-making were also significantly associated with a suicide attempt.
This study contributes to the existing literature on risk factors for suicidal symptomatology in depressed women. Identifying specific depressive symptoms and co-morbid psychiatric disorders may help improve the clinical assessment of suicide risk in depressed patients. These findings could be helpful in identifying those who need more intense treatment strategies in order to prevent suicide.
An immuno-capture polymerase chain reaction (IC-PCR) method for detection of Acidovorax avenae subsp. citrulli (AAC), the causal organism of bacterial fruit blotch (BFB) of watermelon, was developed by combining the immunosorbent enrichment (ISE) method with classical PCR and comparing with the direct PCR and growth check methods. The results showed that all A. avenae subsp. citrulli strains tested have produced 360 bp specific fragments using IC-PCR and direct PCR methods, while other strains from 10 different genera showed negative PCR results. The minimum detection concentration was about 50–100 cfu/ml and 104 cfu/ml, respectively. The IC-PCR sensitivity was 100 times higher than that of direct PCR. The examination of seven batches of different melon seeds from the markets by IC-PCR showed that one cantaloupe, two honeydew melon and two watermelon seed varieties carried the pathogen, indicating that the IC-PCR is an accurate, sensitive, rapid and low-cost technique.
A set of 146 representative adzuki (Vigna angularis var. angularis and var. nipponensis) germplasm from six Asian countries with a tradition of adzuki bean production, together with an outgroup standard rice bean (Vigna umbellata), were analysed by amplified fragment length polymorphism (AFLP) methodology using 12 informative primer pairs. A total of 313 unambiguous polymorphic bands were created. According to the dendrogram, using cluster analysis based on AFLP banding, 143 of the accessions were distinct and revealed enough genetic diversity for identification and classification of accessions within Vigna angularis. A neighbour-joining tree was generated using the newly developed Innan's nucleotide diversity estimate from the AFLP data. From analysis, seven distinct evolutionary groups, named ‘Chinese cultivated’, ‘Japanese cultivated’, ‘Japanese complex-Korean cultivated’, ‘Chinese wild’, ‘China Taiwan wild’, ‘Nepal- Bhutan cultivated’ and ‘Himalayan wild’, were detected. Nucleotide diversity with geographical distribution of each group is discussed, regarding the evolutionary relationships between wild and cultivated adzuki beans. The preliminary results indicated that cultivated adzuki beans have been domesticated from at least four progenitors with at least three geographical origins.
Benign chondroma affecting the soft tissues is uncommon. This tumour is particularly rare in the neck. We present the first case of soft tissue chondroma arising in the parapharyngeal space of a 20-year-old man with a two-year history of a gradual sensation of a swelling in the pharynx with increasing snoring. The CT and pathological features of this lesion will be discussed. We suggest that a peroral approach should be considered for benign parapharyngeal tumour which has been demonstrated on imaging to be in the anterior parapharyngeal space, especially for tumours that are medial to the pterygoid muscles even though the tumour is a large one.
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