The numerical investigation focuses on the flow patterns around a rectangular cylinder with three aspect ratios ($L/D=5$, $10$, $15$) at a Reynolds number of $1000$. The study delves into the dynamics of vortices, their associated frequencies, the evolution of the boundary layer and the decay of the wake. Kelvin–Helmholtz (KH) vortices originate from the leading edge (LE) shear layer and transform into hairpin vortices. Specifically, at $L/D=5$, three KH vortices merge into a single LE vortex. However, at $L/D=10$ and $15$, two KH vortices combine to form a LE vortex, with the rapid formation of hairpin vortex packets. A fractional harmonic arises due to feedback from the split LE shear layer moving upstream, triggering interaction with the reverse flow. Trailing edge (TE) vortices shed, creating a Kármán-like street in the wake. The intensity of wake oscillation at $L/D=5$ surpasses that in the other two cases. Boundary layer transition occurs after the saturation of disturbance energy for $L/D=10$ and $15$, but not for $L/D=5$. The low-frequency disturbances are selected to generate streaks inside the boundary layer. The TE vortex shedding induces the formation of a favourable pressure gradient, accelerating the flow and fostering boundary layer relaminarization. The self-similarity of the velocity defect is observed in all three wakes, accompanied by the decay of disturbance energy. Importantly, the decrease in the shedding frequency of LE (TE) vortices significantly contributes to the overall decay of disturbance energy. This comprehensive exploration provides insights into complex flow phenomena and their underlying dynamics.

The domestication of rice increases the divergence between cultivated rice and its wild progenitor because of artificial selection. However, it remains unknown whether highly diverged loci in rice can be explained by neutral demographic scenarios alone. In this study, we genotyped 45 InDels (insertion/deletion) in two subspecies of Asian cultivated rice (Oryza sativa ssp. japonica and Oryza sativa ssp. indica) and their wild progenitor (O. rufipogon/O. nivara). Among them, 17 loci are highly diverged (FST > 0.4) between rice cultivars and their ancestor. We performed coalescent-based simulations on neutral demographic scenarios and found that neutral demography alone could explain the polymorphic profiles on those highly diverged loci between cultivated and wild rice. Therefore, more signatures of selection should be considered when detecting artificial selection in rice.

Direct numerical simulations are conducted for temporally evolving stratified wake flows at Reynolds numbers from $10\,000$ to $50\,000$ and Froude numbers from $2$ to 50. Unlike previous studies that obtained statistics from a single realization, we take ensemble averages among 80–100 realizations. Our analysis shows that data from one realization incur large convergence errors. These errors reduce quickly as the number of statistical samples increases, with the benefit of ensemble average diminishing beyond 40–60 realizations. The data with ensemble average allow us to test the previously established scalings and arrive at new scaling estimates. Specifically, the data do not support power-law scaling in the centreline velocity deficit $U_0$ beyond the near wake. Its decay rate increases continuously from 0.1 at the onset of the non-equilibrium regime until the end of our calculations without reaching any asymptote. Additionally, while no power-law scalings could be found in the wake width ($L_H$) and wake height ($L_V$) in the late wake, $L_H\sim (Nt)^{1/3}$ is a good working approximation of the wake's horizontal size, where $N$ is buoyancy frequency and $t$ is time. Besides the low-order statistics, we also report the transverse integrated terms and the vertically integrated terms in the turbulent kinetic energy budget equation as a function of the vertical and transverse coordinates. The data indicate that there are two peaks in the vertically integrated production and transport terms, and one peak when the two terms are integrated horizontally.

Microporous structure in zeolite leads to diffusion limitation, which causes coke formation and is harmful to catalytic reactions. Hierarchical zeolite containing primary microporosity and secondary porosity at the meso- and macroscales has received much attention due to its enhanced mass transport. Hierarchical Y zeolites were obtained by treating NH4-Y zeolite with high-temperature calcining and acid-base leaching. The results demonstrated that the calcined zeolite showed great crystallinity after acid-base leaching. The mechanism of introduction of mesopores was demonstrated in detail. The calcination transformed framework Al to extra-framework Al and enlarged the defect by acid and alkali. The mesopores increased with the calcining temperature and holding time. The relationship between the heating rate and the removal of Al species was non-linear; a heating rate of 100°C/h exhibited good protection for the zeolite structure.

Glioma is a highly aggressive form of brain cancer characterized by limited treatment options and poor patient prognosis. In this study, we aimed to elucidate the oncogenic role of thymosin beta-10 (TMSB10) in glioma through comprehensive analyses of patient data from the TCGA and GTEx databases. Our investigation encompassed several key aspects, including the analysis of patients’ clinical characteristics, survival analysis, in vitro and in vivo functional experiments, and the exploration of correlations between TMSB10 expression and immune cell infiltration. Our findings revealed a significant upregulation of TMSB10 expression in glioma tissues compared to normal brain tissues, with higher expression levels observed in tumors of advanced histological grades. Moreover, we observed positive correlations between TMSB10 expression and patient age, while no significant association with gender was detected. Additionally, TMSB10 exhibited marked elevation in gliomas with wild-type IDH and noncodeletion of 1p/19q. Survival analysis indicated that high TMSB10 expression was significantly associated with worse overall survival, disease-specific survival, and progression-free survival in glioma patients. Functionally, knockdown of TMSB10 in glioma cells resulted in reduced cellular growth rates and impaired tumor growth in xenograft models. Furthermore, our study revealed intriguing correlations between TMSB10 expression and immune cell infiltration within the tumor microenvironment. Specifically, TMSB10 showed negative associations with plasmacytoid dendritic cells (pDC) and γδ T cells (Tgd), while displaying positive correlations with neutrophils and macrophages. These findings collectively provide valuable insights into the oncogenic properties of TMSB10 in glioma, suggesting its potential as a therapeutic target and a biomarker for patient stratification.

LongYan kaolin has a large Fe content which affects the coloring. Bioleaching treatments to remove Fe impurities were conducted here using indigenous dissimilatory Fe(III)-reducing bacteria. The factors that affect bioleaching efficiency include bioleaching time, carbon source, pH, temperature, pulp density, and inoculum density and these were examined. Environmental scanning electron microscopy and X-ray diffraction were used to examine any textural or mineralogical changes at the surface of the kaolin that may have occurred during the bioleaching. Iron impurities in the kaolin were reduced from 0.88% to 0.48% with an increase in the natural whiteness index from 60.8% to 81.5% after 7 days of bioleaching treatment. A granulometric analysis of dispersed kaolin demonstrated that the bioleaching resulted in a decrease in particle size. The results demonstrated that the bioleaching was very effective at improving the quality of the kaolin, where insoluble Fe(III), either adsorbed to the kaolin surfaces or admixed as amorphous forms, was leached out by micro-organisms as water-soluble Fe(II).

The clay mineral content of the Oligocene sediments in the Linxia Basin has been investigated using X-ray diffraction, scanning electron microscopy, and high-resolution transmission electron microscopy. The clay mineral assemblages are mainly mixed-layer illite-smectite (I-S), illite, kaolinite and minor palygorskite in the early-Middle Oligocene deposits, mixed-layer I-S, illite and kaolinite in the Middle Oligocene deposits, mainly illite and chlorite (usually >50–70 vol.%), mixed-layer I-S, and trace to minor palygorskite in the late Oligocene sediments, respectively. The mineral assemblage indicates a warm and seasonally humid climate in the Middle Oligocene, with an episode of warm and dry conditions in the early stage of the Middle Oligocene, and a trend of temperature decrease and more arid conditions in the late Oligocene. Climate evolution in the Oligocene corresponds with the significant elevation change in central Tibet since late Oligocene, and therefore, suggests that tectonic-forced cooling of climate took place in Linxia in the northeast margin of the Tibetan Plateau. The ubiquitous mixed-layer I-S and carbonates throughout the Oligocene sediments reflect relatively small fluctuations in climate conditions during the epoch. The changes in clay mineral components and feldspars in the late Oligocene suggest a variation in the source of clastic materials, which probably reflects an increase in erosion of soils and poorly weathered parent rock in more elevated or high-relief source areas during this period of tectonic uplift.

With the development of high-power microwave technology, the output power of the pulse generator is required more and more higher. In this paper, it is realized by increasing the output power of the module while the output impedance of the module changes little. The module of the generator is based on pulse forming network (PFN) and linear transformer (LT). Four Blumlein PFNs with arc-type configuration and 24 Ω characteristic impedance were connected symmetrically to the primary coil of the LTD and driven by two identical laser triggered spark switches to ensure four Blumlein PFNs synchronizing operation. On this basis, a two-stage high-power pulse generator based on PFN-LT is developed. The following technical parameters of the generator were achieved on a 12 Ω high-power solid resistor: output voltage amplitude of ∼250 kV and output power of ∼5.2 GW at a repetition rate of 5 Hz.

Femtosecond laser ablation is widely applied in high-precision machining of microholes in aeroengine turbine blades. To further explore the mechanism of action during the laser processing of microholes, numerical simulations were performed on the basis of a molecular dynamics (MD) method coupled with a two-temperature model (TTM). Laser irradiation on the surface of copper for different femtosecond-laser processing parameters is investigated in this work. Through the femtosecond-laser single-pulse central ablation simulation model, the laser energy flux density in a Gaussian laser spot range was discretized and analyzed to calculate the ablation depth at multiple points separately. The cross-sectional morphology of the femtosecond-laser single-pulse ablation pits was approximated and fitted. Finally, a 3D simulation model of the whole process of multiscale femtosecond-laser spiral processing microholes was established by superimposing multipulse femtosecond-laser spiral trajectories. This provides a theoretical basis for analyzing the evolution of geometric parameters and morphological characteristics of the hole during machining with specific laser and process parameters.

Non-alcoholic fatty liver disease (NAFLD) is a prevalent liver disorder, affecting approximately 25 % of the population. Coffee-drinking obese smokers exhibit lower body weights and decreased NAFLD rates, but the reasons behind this remain unclear. Additionally, the effect of nicotine, the main component of tobacco, on the development of NAFLD is still controversial. Our study aimed to explore the possible reasons that drinking coffee could alleviate NAFLD and gain weight and identify the real role of nicotine in NAFLD of obese smokers. A NAFLD model in mice was induced by administering nicotine and a high-fat diet (HFD). We recorded changes in body weight and daily food intake, measured the weights of the liver and visceral fat, and observed liver and adipose tissue histopathology. Lipid levels, liver function, liver malondialdehyde (MDA), superoxide dismutase (SOD), serum inflammatory cytokine levels and the expression of hepatic genes involved in lipid metabolism were determined. Our results demonstrated that nicotine exacerbated the development of NAFLD and caffeine had a hepatoprotective effect on NAFLD. The administration of caffeine could ameliorate nicotine-plus-HFD-induced NAFLD by reducing lipid accumulation, regulating hepatic lipid metabolism, alleviating oxidative stress, attenuating inflammatory response and restoring hepatic functions. These results might explain why obese smokers with high coffee consumption exhibit the lower incidence rate of NAFLD and tend to be leaner. It is essential to emphasise that the detrimental impact of smoking on health is multifaceted. Smoking cessation remains the sole practical and effective strategy for averting the tobacco-related complications and reducing the risk of mortality.

This study presents novel findings on stochastic electron heating via a random electron cyclotron wave (ECW) in a spherical tokamak. Hard x ray measurements demonstrate the time evolution of hard x ray counts at different energy bands, consistent with predictions from the stochastic heating model. The ECW heating rate shows a positive correlation with applied power, confirming the effectiveness of stochastic heating. Remarkably, the ECW-driven plasma current remains insensitive to ECW incidence angle, consistent with model predictions. The observed stochastic heating of electrons offers potential for exploring innovative non-inductive current drive modes in spherical tokamaks. This research contributes to the understanding of plasma behaviour and motivates the development of new models for non-inductive current drive in fusion devices.

This study aimed to investigate the optimal frozen embryo transfer (FET) strategy for recurrent implantation failure (RIF) patients with three consecutive failed cleaved embryo implantations and no blastocyst preservation. This retrospective analysis was divided into three groups based on the FET strategy: thawed day 3 embryo transfer (D3 FET group); and extended culture of frozen–thawed day 3 embryos to day 5 blastocysts transfer (D3–D5 FET group); thawed blastocyst transfer (D5 FET group). Transplant cycle data were compared between the three groups. In total, 43.8% of vitrified–thawed cleavage embryos developed into blastocysts. Analysis of the three transplantation strategies showed that, compared with the D3 FET group, D3–D5 had a significantly better hCG-positivity rate and live-birth rate (P < 0.05). Pregnancy outcomes in the D3–D5 FET group and D5 FET group were similar regarding hCG-positivity rate, implantation rate, clinical pregnancy rate, and live-birth rate. Our findings propose two potentially valuable transfer strategies for patients experiencing repeated implantation failures. The D3–D5 FET approach presents a greater potential for selecting promising embryos in cases without blastocyst preservation; however, this strategy does entail the risk of cycle cancellation. Conversely, in instances where blastocyst preservation is an option, prioritizing consideration of the D5 FET strategy is recommended.

Carotenoids are important bioactive substances in breast milk, the profile of which is seldom studied. This study aimed to explore the profile of carotenoids in breast milk and maternal/cord plasma of healthy mother–neonate pairs in Shanghai, China, and their correlation with dietary intake. Maternal blood, umbilical cord blood and breast milk samples from five lactation stages (colostrum, transitional milk and early-, mid- and late-term mature milk) were collected. Carotenoid levels were analysed by HPLC. Carotenoid levels in breast milk changed as lactation progressed (P < 0·001). β-Carotene was the primary carotenoid in colostrum. Lutein accounted for approximately 50 % of total carotenoids in transitional milk, mature milk and cord blood. Positive correlations were observed between five carotenoids in umbilical cord blood and maternal blood (P all < 0·001). β-Carotene levels were also correlated between maternal plasma and three stages of breast milk (r = 0·605, P < 0·001; r = 0·456, P = 0·011, r = 0·446; P = 0·013, respectively). Dietary carotenoid intakes of lactating mothers also differed across lactation stages, although no correlation with breast milk concentrations was found. These findings suggest the importance of exploring the transport mechanism of carotenoids between mothers and infants and help guide the development of formulas for Chinese infants as well as the nutritional diets of lactating mothers.

Sterol regulatory element-binding protein 2 (SREBP2) is considered to be a major regulator to control cholesterol homoeostasis in mammals. However, the role of SREBP2 in teleost remains poorly understand. Here, we explored the molecular characterisation of SREBP2 and identified SREBP2 as a key modulator for 3-hydroxy-3-methylglutaryl-coenzyme A reductase and 7-dehydrocholesterol reductase, which were rate-limiting enzymes of cholesterol biosynthesis. Moreover, dietary palm oil in vivo or palmitic acid (PA) treatment in vitro elevated cholesterol content through triggering SREBP2-mediated cholesterol biosynthesis in large yellow croaker. Furthermore, our results also found that PA-induced activation of SREBP2 was dependent on the stimulating of endoplasmic reticulum stress (ERS) in croaker myocytes and inhibition of ERS by 4-Phenylbutyric acid alleviated PA-induced SREBP2 activation and cholesterol biosynthesis. In summary, our findings reveal a novel insight for understanding the role of SREBP2 in the regulation of cholesterol metabolism in fish and may deepen the link between dietary fatty acid and cholesterol biosynthesis.