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Resolving Electrode-Electrolyte Interfaces in Batteries with Low Dose Cryogenic Transmission Electron Microscopy
Zewen Zhang, Yuzhang Li, Weijiang Zhou, Yanbin Li, Wah Chiu, Yi Cui
Journal: Microscopy and Microanalysis / Volume 28 / Issue S1 / August 2022
Published online by Cambridge University Press: 22 July 2022, pp. 2204-2205
Print publication: August 2022
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Cryo-EM Information Management System and Sample Evaluation at Stanford-SLAC Cryo-EM Center
Htet Khant, Yee T. Li, Murali Shankar, Corey Hecksel, Patrick Mitchell, Yan Liu, Lydia-Marie Joubert, Chensong Zhang, Lisa Dunn, Michael Schmid, Britt Hedman, Wah Chiu
Journal: Microscopy and Microanalysis / Volume 28 / Issue S1 / August 2022
Published online by Cambridge University Press: 22 July 2022, pp. 1304-1305
Print publication: August 2022
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High Resolution Data Collection at S2C2, a National CryoEM Center
Corey Hecksel, Kaiming Zhang, Grigore Pintilie, Patrick Mitchell, Yee-Ting Li, Wah Chiu
Journal: Microscopy and Microanalysis / Volume 27 / Issue S1 / August 2021
Published online by Cambridge University Press: 30 July 2021, pp. 1152-1154
Print publication: August 2021
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Resolve cathode electrolyte interphase in lithium batteries with cryo-EM
Zewen Zhang, Jinlong Yang, William Huang, Hansen Wang, Weijiang Zhou, Yanbin Li, Yuzhang Li, Jinwei Xu, Wenxiao Huang, Wah Chiu, Yi Cui
Journal: Microscopy and Microanalysis / Volume 27 / Issue S1 / August 2021
Published online by Cambridge University Press: 30 July 2021, pp. 2188-2190
Print publication: August 2021
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Explore the complexity of proteins with an expanded CryoET data processing pipeline
Muyuan Chen, David Chmielewski, Wah Chiu, Steven Ludtke
Journal: Microscopy and Microanalysis / Volume 27 / Issue S1 / August 2021
Published online by Cambridge University Press: 30 July 2021, pp. 2816-2817
Print publication: August 2021
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CryoEM Map-Model Scores: From Average Density to Q-scores
Grigore Pintilie, Wah Chiu
Journal: Microscopy and Microanalysis / Volume 27 / Issue S1 / August 2021
Published online by Cambridge University Press: 30 July 2021, pp. 1382-1384
Print publication: August 2021
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Sub-Ångström-resolution MicroED Using a Direct Detection Camera
Benjamin Bammes, Weijiang Zhou, Wah Chiu
Journal: Microscopy and Microanalysis / Volume 26 / Issue S2 / August 2020
Published online by Cambridge University Press: 30 July 2020, pp. 1524-1526
Print publication: August 2020
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A 3.4-Å cryo-electron microscopy structure of the human coronavirus spike trimer computationally derived from vitrified NL63 virus particles
Kaiming Zhang, Shanshan Li, Grigore Pintilie, David Chmielewski, Michael F. Schmid, Graham Simmons, Jing Jin, Wah Chiu
Journal: QRB Discovery / Volume 1 / 2020
Published online by Cambridge University Press: 17 November 2020, e11
Print publication: 2020
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Human coronavirus NL63 (HCoV-NL63) is an enveloped pathogen of the family Coronaviridae that spreads worldwide and causes up to 10% of all annual respiratory diseases. HCoV-NL63 is typically associated with mild upper respiratory symptoms in children, elderly and immunocompromised individuals. It has also been shown to cause severe lower respiratory illness. NL63 shares ACE2 as a receptor for viral entry with SARS-CoV-1 and SARS-CoV-2. Here, we present the in situ structure of HCoV-NL63 spike (S) trimer at 3.4-Å resolution by single-particle cryo-EM imaging of vitrified virions without chemical fixative. It is structurally homologous to that obtained previously from the biochemically purified ectodomain of HCoV-NL63 S trimer, which displays a three-fold symmetric trimer in a single conformation. In addition to previously proposed and observed glycosylation sites, our map shows density at other sites, as well as different glycan structures. The domain arrangement within a protomer is strikingly different from that of the SARS-CoV-2 S and may explain their different requirements for activating binding to the receptor. This structure provides the basis for future studies of spike proteins with receptors, antibodies or drugs, in the native state of the coronavirus particles.

Evaluation of an in-house self-held respiration monitoring device for deep inspiration breath hold techniques for radiotherapy of patients with cancer of the left breast
Yuen Yan Chan, Ki Man Ku, Yin Ping Ng, Siu Ki Ben Yu, Chi Wah Tony Kong, Wing Lun Alan Mui, Chun Man Cornel Wong, Sze Wan Kong, Sze Ming Wong, George Chiu
Journal: Journal of Radiotherapy in Practice / Volume 19 / Issue 2 / June 2020
Published online by Cambridge University Press: 09 August 2019, pp. 122-126
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Background:
An in-house self-held respiration monitoring device (SHRMD) was developed for providing deep inspiration breath hold (DIBH) radiotherapy. The use of SHRMD is evaluated in terms of reproducibility, stability and heart dose reduction.
Methods and materials:
Sixteen patients receiving radiotherapy of left breast cancer were planned for treatment with both a free breathing (FB) scan and a DIBH scan. Both FB and DIBH plans were generated for comparison of the heart, left anterior descending (LAD) artery and lung dose. All patients received their treatments with DIBH using SHRMD. Megavoltage cine images were acquired during treatments for evaluating the reproducibility and stability of treatment position using SHRMD.
Results:
Compared with FB plans, the maximum dose to the heart by DIBH technique with SHRMD was reduced by 29·9 ± 15·6%; and the maximum dose of the LAD artery was reduced by 41·6 ± 18·3%. The inter-fractional overall mean error was 0·01 cm and the intra-fractional overall mean error was 0·04 cm.
Conclusion:
This study demonstrated the potential benefits of using the SHRMD for DIBH to reduce the heart and LAD dose. The patients were able to perform stable and reproducible DIBHs.

Cryo-EM Study of Chaperonin Mm-Cpn's Conformational Heterogeneity under Different ATP Conditions
Yanyan Zhao, Wah Chiu
Journal: Microscopy and Microanalysis / Volume 25 / Issue S2 / August 2019
Published online by Cambridge University Press: 05 August 2019, pp. 1006-1007
Print publication: August 2019
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Stanford-SLAC Cryo-EM Center (S2C2)
Wah Chiu, Yee Ting Li, David Bushnell, Michael F. Schmid, Georgios Skiniotis, Britt Hedman
Journal: Microscopy and Microanalysis / Volume 25 / Issue S2 / August 2019
Published online by Cambridge University Press: 05 August 2019, pp. 2658-2659
Print publication: August 2019
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Chapter 11 - How to Manage IVF Patients with Intrauterine Adhesions
- By Jacqueline Chung, Pui Wah, Tin-Chiu Li
Edited by Ying Cheong, University of Southampton, Togas Tulandi, McGill University, Montréal, Tin-Chiu Li, The Chinese University of Hong Kong
Book: Practical Problems in Assisted Conception
Published online: 22 August 2018
Print publication: 20 September 2018, pp 40-45
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GENFIRE: from Precisely Localizing Single Atoms in Materials to High Resolution 3D Imaging of Cellular Structures
Jianwei Miao, Alan Pryor, Jr., Yongsoo Yang, Arjun Rana, Marcus Gallagher-Jones, Jihan Zhou, Yuan Hung Lo, Jose A. Rodriguez, Wah Chiu
Journal: Microscopy and Microanalysis / Volume 24 / Issue S1 / August 2018
Published online by Cambridge University Press: 01 August 2018, pp. 1446-1447
Print publication: August 2018
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Going Deeper in Cryo Electron Tomography with Neural Networks
Muyuan Chen, Wei Dai, Stella Y. Sun, Michael F. Schmid, Wah Chiu, Steven J. Ludtke
Journal: Microscopy and Microanalysis / Volume 23 / Issue S1 / July 2017
Published online by Cambridge University Press: 04 August 2017, pp. 814-815
Print publication: July 2017
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Quantifying Variability of Manual Annotation in Cryo-Electron Tomograms
Corey W. Hecksel, Michele C. Darrow, Wei Dai, Jesús G. Galaz-Montoya, Jessica A. Chin, Patrick G. Mitchell, Shurui Chen, Jemba Jakana, Michael F. Schmid, Wah Chiu
Journal: Microscopy and Microanalysis / Volume 22 / Issue 3 / June 2016
Published online by Cambridge University Press: 26 May 2016, pp. 487-496
Print publication: June 2016
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Although acknowledged to be variable and subjective, manual annotation of cryo-electron tomography data is commonly used to answer structural questions and to create a “ground truth” for evaluation of automated segmentation algorithms. Validation of such annotation is lacking, but is critical for understanding the reproducibility of manual annotations. Here, we used voxel-based similarity scores for a variety of specimens, ranging in complexity and segmented by several annotators, to quantify the variation among their annotations. In addition, we have identified procedures for merging annotations to reduce variability, thereby increasing the reliability of manual annotation. Based on our analyses, we find that it is necessary to combine multiple manual annotations to increase the confidence level for answering structural questions. We also make recommendations to guide algorithm development for automated annotation of features of interest.

IP3R1 - Assessing Map Interpretability at Near Atomic Resolution
Steven J. Ludtke, Guizhen Fan, Matthew L. Baker, Zhao Wang, Mariah R. Baker, Stephen Murray, Pavel A. Synyagovsky, Wah Chiu, Irina I. Serysheva
Journal: Microscopy and Microanalysis / Volume 21 / Issue S3 / August 2015
Published online by Cambridge University Press: 23 September 2015, pp. 543-544
Print publication: August 2015
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Modeling Protein Structure in Macromolecular Assemblies at Near Atomic Resolutions
Matthew L. Baker, Muyuan Chen, Philip R. Baldwin, Steven J. Ludtke, Wah Chiu
Journal: Microscopy and Microanalysis / Volume 21 / Issue S3 / August 2015
Published online by Cambridge University Press: 23 September 2015, pp. 541-542
Print publication: August 2015
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Optimization of JEM2200FS for Zernike Phase Contrast Cryo-EM
Htet A. Khant, Caroline Fu, Sohei Motoki, Matthew H. Sullivan, Guy DeRose, Wah Chiu
Journal: Microscopy and Microanalysis / Volume 21 / Issue S3 / August 2015
Published online by Cambridge University Press: 23 September 2015, pp. 1577-1578
Print publication: August 2015
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Zernike Phase Plate Configuration at Intermediate Lens Position on JEM2200FS
Wah Chiu, Caroline J.-Y. Fu, Htet Khant, Sohei Motoki
Journal: Microscopy and Microanalysis / Volume 21 / Issue S3 / August 2015
Published online by Cambridge University Press: 23 September 2015, pp. 2143-2144
Print publication: August 2015
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Visualizing Virus Assembly Intermediates Inside Marine Cyanobacteria by Zernike Phase Contrast Electron Cryo-Tomography
Wah Chiu, Wei Dai, Caroline Fu, Desislava Raytcheva, John Flanagan, Htet A. Khant, Xiangan Liu, Ryan H. Rochat, Cameron Haase-Pettingell, Jacqueline Piret, Steve J. Ludtke, Kuniaki Nagayama, Michael F. Schmid, Jonathan A. King
Journal: Microscopy and Microanalysis / Volume 20 / Issue S3 / August 2014
Published online by Cambridge University Press: 27 August 2014, pp. 202-203
Print publication: August 2014
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38 results

Resolving Electrode-Electrolyte Interfaces in Batteries with Low Dose Cryogenic Transmission Electron Microscopy

Cryo-EM Information Management System and Sample Evaluation at Stanford-SLAC Cryo-EM Center

High Resolution Data Collection at S2C2, a National CryoEM Center

Resolve cathode electrolyte interphase in lithium batteries with cryo-EM

Explore the complexity of proteins with an expanded CryoET data processing pipeline

CryoEM Map-Model Scores: From Average Density to Q-scores

Sub-Ångström-resolution MicroED Using a Direct Detection Camera

A 3.4-Å cryo-electron microscopy structure of the human coronavirus spike trimer computationally derived from vitrified NL63 virus particles

Evaluation of an in-house self-held respiration monitoring device for deep inspiration breath hold techniques for radiotherapy of patients with cancer of the left breast

Cryo-EM Study of Chaperonin Mm-Cpn's Conformational Heterogeneity under Different ATP Conditions

Stanford-SLAC Cryo-EM Center (S2C2)

Chapter 11 - How to Manage IVF Patients with Intrauterine Adhesions

GENFIRE: from Precisely Localizing Single Atoms in Materials to High Resolution 3D Imaging of Cellular Structures

Going Deeper in Cryo Electron Tomography with Neural Networks

Quantifying Variability of Manual Annotation in Cryo-Electron Tomograms

IP3R1 - Assessing Map Interpretability at Near Atomic Resolution

Modeling Protein Structure in Macromolecular Assemblies at Near Atomic Resolutions

Optimization of JEM2200FS for Zernike Phase Contrast Cryo-EM

Zernike Phase Plate Configuration at Intermediate Lens Position on JEM2200FS

Visualizing Virus Assembly Intermediates Inside Marine Cyanobacteria by Zernike Phase Contrast Electron Cryo-Tomography

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38 results