Random amplification of polymorphic DNA (RAPD), using both fingerprinting
(M13, [GTG]5, [GACA]4) and random
has been used to analyse 145 isolates characteristic of Trichoderma
sect. Longibrachiatum and Hypocrea species with anamorphs
referable to that section, and to identify strains with named species of
sect. Longibrachiatum. To this end, ex-type strains of T.
longibrachiatum, T. pseudokoningii, T. citrinoviride,
parceramosum and T. reesei were analysed, and the similarity
of RAPD characters used as a criterion for the alignment of strains to
species. The ex-type strains of T. saturnisporum and T.
ghanense exhibited the same interspecific similarity index as the
ex-type strains of Trichoderma sect. Longibrachiatum,
clustered well within it and were therefore included into this section.
isolates were identified as members of Trichoderma sect.
Longibrachiatum and further investigated. Most of the anamorphic
could be aligned with T. citrinoviride or T. longibrachiatum,
whereas only a few strains of T. saturnisporum and T. parceramosum
were found. No naturally occurring anamorphs with homology
to the ex-type strains of T. reesei and T. pseudokoningii
found. While T. longibrachiatum and T. citrinoviride
through much of their geographic ranges, the former was present in Africa
India but not in southeast Asia, while the reverse was true of
the latter. The African strains of T. longibrachiatum were equally
distant from the ex-type strains of T. longibrachiatum, T.
parceramosum and T. saturnisporum. The type strain of T.
ghanense was identified as belonging to this group. T. pseudokoningii,
the anamorph of Hypocrea schweinitzii, was found exclusively in
Australia and New Zealand. Attempts to identify potential
teleomorphs of the other anamorphs revealed another subgroup of
H. schweinitzii, occurring in temperate climates in Europe and
U.S.A., as homologous to T. citrinoviride. No Hypocrea
could be identified as teleomorphs of T. longibrachiatum,
T. parceramosum or T. saturnisporum.