Bayesian analysis of radiocarbon (14C) dates in North American archaeology is increasing, especially among archaeologists working in deeper time. However, historical archaeologists have been slow to embrace these new techniques, and there have been only a few examples of the incorporation of calendar dates as informative priors in Bayesian models in such work in the United States. To illustrate the value of Bayesian approaches to sites with both substantial earlier Native American occupations as well as a historic era European presence, we present the results of our Bayesian analysis of 14C dates from the earlier Guale village and the Mission period contexts from the Sapelo Shell Ring Complex (9MC23) in southern Georgia. Jefferies and Moore have explored the Spanish Mission period deposits at this site to better understand the Native American interactions with the Spanish during the 16th and 17th centuries along the Georgia Coast. Given the results of our Bayesian modeling, we can say with some degree of confidence that the deposits thus far excavated and sampled contain important information dating to the 17th-century mission on Sapelo Island. In addition, our modeling of new dates suggests the range of the pre-Mission era Guale village. Based on these new dates, we can now say with some degree of certainty which of the deposits sampled likely contain information that dates to one of the critical periods of Mission period research, the AD 1660–1684 period that ushered in the close of mission efforts on the Georgia Coast.

Farmers’ access to reliable information is crucial to improving rural livelihoods, food security, and national economies in West Africa. This paper discusses the dynamics of accessing and using agricultural learning videos from commercial channels, vs project and non-project channels in Benin and Mali. Using combinations of different models to assess the effectiveness of agricultural extension programs, the findings showed that farmers were motivated to pay for videos and watch them by themselves, without facilitation. Farmers who watched the videos through project support have also continued to watch on their own if the videos are of interest to them. Nevertheless, farmers were less motivated in the learning process when they received the Digital Video Disc (DVD) free and without support to watch them. We also found that the distribution of learning videos through commercial channels reaches more serious users and increases farmers’ self-determination for learning, and farmers are more motivated to provide feedback than viewers who receive DVDs for free or via project support, Non-Governmental Organizations (NGOs), or farmer organizations. Although buying a DVD is an individual action, they like to watch the videos in groups. After buying the DVD, about 43% of respondent borrowed DVD players and one person in five bought a DVD player to watch the videos. Efforts to promote improved technologies need to expand beyond the conventional focus on research and extension services. Support to agricultural technology dissemination must go beyond assistance to smallholder farmers and NGOs (practical implication). As the private sector has a role to play, both in making technologies available and in teaching farmers how to use them, their contribution would create space for innovation (theoretical implication). Our findings suggest that successful development intervention programs can be sell audiovisual material to farmers, who will use it proactively.

Photoreceptors have high energy demands and densely packed mitochondria through which light passes before phototransduction. Old world primates including humans have three cone photoreceptor types mediating color vision with short (S blue), medium (M green), and long (L red) wavelength sensitivities. However, S-cones are enigmatic. They comprise <10% of the total cone population, their responses saturate early, and they are susceptible in aging and disease. Here, we show that primate S-cones actually have few mitochondria and are fueled by glycolysis, not by mitochondrial respiration. Glycolysis has a limited ability to sustain activity, potentially explaining early S-cone saturation. Mitochondria act as optical filters showing reduced light transmission at 400–450 nm where S-cones are most sensitive (420 nm). This absorbance is likely to arise in a mitochondrial porphyrin that absorbs strongly in the Soret band. Hence, reducing mitochondria will improve S-cone sensitivity but result in increased glycolysis as an alternative energy source, potentially increasing diabetic vulnerability due to restricted glucose access. Further, glycolysis carries a price resulting in premature functional decline as seen in aged S-cones. Soret band absorption may also impact on mitochondrial rich M and L cones by reducing sensitivity at the lower end of their spectral sensitivity range resulting in increased differentiation from S-cone responses. These data add to the list of unique characteristic of S-cones and may also explain aspects of their vulnerability.

OBJECTIVES/SPECIFIC AIMS: Rodent models can be used to study neonatal abstinence syndrome (NAS), but the applicability of findings from the models to NAS in humans is not well understood. The objective of this study was to develop a rat model of norbuprenorphine-induced NAS and validate its translational value by comparing blood concentrations in the norbuprenorphine-treated pregnant rat to those previously reported in pregnant women undergoing buprenorphine treatment. METHODS/STUDY POPULATION: Pregnant Long-Evans rats were implanted with 14-day osmotic minipumps containing vehicle, morphine (positive control), or norbuprenorphine (0.3–3 mg/kg/d) on gestation day 9. Within 12 hours of delivery, pups were tested for spontaneous or precipitated opioid withdrawal by injecting them with saline (10 mL/kg, i.p.) or naltrexone (1 or 10 mg/kg, i.p), respectively, and observing them for well-validated neonatal withdrawal signs. Blood was sampled via indwelling jugular catheters from a subset of norbuprenorphine-treated dams on gestation day 8, 10, 13, 17, and 20. Norbuprenorphine concentrations in whole blood samples were quantified using LC/MS/MS. RESULTS/ANTICIPATED RESULTS: Blood concentrations of norbuprenorphine in rats exposed to 1–3 mg/kg/d of norbuprenorphine were similar to levels previously reported in pregnant women undergoing buprenorphine treatment. Pups born to dams treated with these doses exhibited robust withdrawal signs. Blood concentrations of norbuprenorphine decreased across gestation, which is similar to previous reports in humans. DISCUSSION/SIGNIFICANCE OF IMPACT: These results suggest that dosing dams with 1–3 mg/kg/day norbuprenorphine produces maternal blood concentrations and withdrawal severity similar to those previously reported in humans. This provides evidence that, at these doses, this model is useful for testing hypotheses about norbuprenorphine that are applicable to NAS in humans.

Glacier basal motion generates diurnal to multi-annual fluctuations in glacier velocity and mass flux. Understanding these fluctuations is important for prediction of future sea-level rise and for gaining insight into glacier physics and erosion. Here, we derive glacier velocity through cross-correlation of WorldView satellite imagery to document the evolution of ice surface velocity on Kennicott Glacier, Alaska, over the 2013 melt season. The summer speedup is spatially uniform over a ~12 km2 area, over which the spring velocity varies significantly. Velocity increases by 1.4-fold to tenfold across the study domain, with larger values where spring velocities are low. To investigate the cross-glacier distribution of basal motion required to explain the observed surface speedup, we employ a two-dimensional cross-sectional glacier flow model. We find the model is insensitive to the spatial distribution of basal slip because stress gradient ice coupling diffuses the surface expression of the basal velocity field. While the temporal evolution of the subglacial hydrologic system is critical for predicting a glacier's response to meltwater inputs, our work suggests that glacier and ice-sheet models do not require a detailed representation of subglacial hydrology to accurately capture the spatial pattern of glacier speedup.

Analyzing historical trajectories of social interactions at varying scales can lead to complementary interpretations of relationships among archaeological settlements. We use social network analysis combined with geographic information systems at three spatial scales over time in the western U.S. Southwest to show how the same social processes affected network dynamics at each scale. The period we address, A.D. 1200–1450, was characterized by migration and demographic upheaval. The tumultuous late thirteenth-century interval was followed by population coalescence and the development of widespread religious movements in the fourteenth and fifteenth centuries. In the southern Southwest these processes resulted in a highly connected network that drew in members of different settlements within and between different valleys that had previously been distinct. In the northern Southwest networks were initially highly connected followed by a more fragmented social landscape. We examine how different network textures emerged at each scale through 50-year snapshots. The results demonstrate the usefulness of applying a multiscalar approach to complex historical trajectories and the potential for social network analysis as applied to archaeological data.

While it is widely believed that a “conservative coalition” of Southern Democrats and Republicans sometimes formed on floor votes in the mid-twentieth-century House to block liberal policy initiatives, a fully fleshed-out picture of what the coalition was and how it operated is lacking. In this article, we investigate whether the conservative coalition wielded negative agenda control, that is, whether it used positions of power in the House to block bills from floor consideration that would have harmed a majority of its members. We find that the likelihood that the conservative coalition was rolled increased significantly after the Rules Committee packing in 1961—especially for bills that came from standing committees chaired by nonsoutherners. These results are consistent with the notion that Rules systematically protected the conservative coalition from unfavorable floor votes and that southern committee chairs continued to offer some blocking power after the Rules packing.