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Effect of lamb age, transport stimulation and repeated hormone superovulation on the number of collected oocytes were determined. Effect of the culture medium containing ethylenediaminetetraacetic acid (EDTA) on the development of embryos produced in vitro from lamb oocytes was also investigated. Results indicated that the mean numbers of collected and available oocytes (oocytes with compacted cumulus cells, evenly granulated ooplasm and morphologically normal) from 6- to 8-week-old lambs were 60.8±13.9 and 58.2±12.3, respectively. These figures were higher than those obtained from 12- to 14-week-old lambs (27.3±5.1 and 26.0±4.9) (P<0.05). Stimulation by transportation didn't decrease the number of collected oocytes from the superovulated lambs (P>0.05). However, the number of collected oocytes in the repeated superovulation group was significantly reduced, compared with the control group (P<0.05). The embryonic culture medium supplemented with 10 μmol/l EDTA highly improved the development capability of the embryos from lamb oocytes (P<0.05). Healthy lambs were born following embryo transfer.
This trial was aimed at investigating the effect of different doses of progesterone (P4) and types of vaginal bolts on new follicular wave induction, oestrus synchronization and superovulation in Holstein heifers. Results showed that the interval after prostaglandin F2α (PGF2α) application and oestrus occurrence between the sponge blot (SP) group (48.3 h) and the progestin release device (PRD) group (39 h) was significantly different (P<0.05). The average number of embryos obtained in the SP group (13.2) was significantly higher than that (8.7) in the PRD group (P<0.05). The same trend was observed for the number of usable embryos (5.7 and 3.3 for SP and PRD groups, respectively). The injection of 50 and 100 mg of P4 efficiently induced a new follicular wave, whereas no significant difference (P>0.05) was recorded on oestrus synchronization and superovulation between the two groups.
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