The continued dispersal of Palmer amaranth can impose detrimental impacts to cropping systems in Wisconsin. Our objective was to characterize the response of a recently introduced Palmer amaranth accession in southern WI to postemergence (POST) and preemergence (PRE) herbicides commonly used in corn and soybean. Greenhouse experiments were conducted with the WI putative herbicide-resistant accession (BRO), and two additional control accessions from Nebraska, a glyphosate-resistant (KEI2) and a glyphosate-susceptible (KEI3). Postemergence treatments were 2,4-D, atrazine, dicamba, glufosinate, glyphosate, imazethapyr, lactofen, and mesotrione at 1× and 3× label rates. Preemergence treatments were atrazine, mesotrione, metribuzin, S-metolachlor, and sulfentrazone at 0.5×, 1×, and 3× label rates. Plant survival of each accession was ≥ 63% after exposure to imazethapyr POST 3× rate. Survival of BRO and KEI2 was 44% (±13) and 50% (±13), respectively, after exposure to atrazine POST 3× rate. Survival of BRO was 69% (±12) after exposure to glyphosate POST 1× rate, whereas survival of KEI2 was 44% (±13) after exposure to glyphosate POST 3× rate. After exposure to 2,4-D POST 1× rate, KEI2 and KEI3 survival was 38% (±13) and 50% (±13), respectively. Survival of all accessions was ≤ 31% after exposure to 2,4-D POST 3× rate, or dicamba, glufosinate, lactofen, and mesotrione POST at either rate. Plant density reduction of KEI2 was 77% (±13) after exposure to atrazine PRE 1× rate, whereas density reduction of BRO was 56% (±13) after exposure to atrazine PRE 3× rate. Plant density reduction of all accessions was ≥ 94% after exposure to mesotrione PRE 1× and 3× rates, or metribuzin, S-metolachlor, and sulfentrazone PRE at either rate. Our results suggest that each accession is resistant (≥ 50% survival) to imazethapyr POST, that BRO and KEI2 are resistant to atrazine and glyphosate POST, and KEI2 and KEI3 are resistant to 2,4-D POST. The recently introduced BRO accession exhibited multiple resistance to imazethapyr, atrazine, and glyphosate POST. In addition, atrazine PRE was ineffective for BRO control, suggesting that diversified resistance management will be critical for its effective management.