Ed Zigler was a champion for underprivileged youth, one who worked alongside communities to fight for long-lasting systemic changes that were informed by his lifespan and ecological perspective on the development of the whole child. This paper reports on the development, implementation, and preliminary outcomes of an intervention that embodied the Zigler approach by adopting a community participatory research lens to integrate complementary insights across community-based providers (promotoras), Latinx immigrant families, and developmental psychologists in the service of promoting parent–child relationship quality and preventing youth aggression and violence. Analyses from the first 112 Latinx mother–youth dyad participants (46% female children, ages 8–17) in the resultant, Confía en mí, Confío en ti, eight-week intervention revealed significant pre–post increases in purported mechanisms of change (i.e., attachment security, reflective functioning) and early intervention outcomes (i.e., depressive, anxiety, and externalizing problems). Treatment responses varied by youth age. A case analysis illustrated the lived experiences of the women and children served by this intervention. We discuss future directions for the program, as well as challenges to its sustainability. Finally, we consider Ed's legacy as we discuss the contributions of this work to developmental science and our understanding of attachment relationships among low-income immigrant Latinx families.

The coast of Aragua is a home of bottlenose dolphins (BND), Atlantic spotted dolphins (ASD) and fishermen (FIS) from four towns. A photo-identification study was carried out on BND to estimate their home ranges. From 2004 to 2008, 100 field surveys were carried out along 30 km of coastline (92.12 km2). In each sighting of BND, information regarding date, time, latitude/longitude and photographs were registered (ASD and FIS were registered without photography). The data were analysed using a Geographic Information System to estimate Minimum Convex Polygon (MCP) and Fixed Kernel (FK) at 95%. The home ranges of BND were estimated for seven individuals. This included three females (29–31 sightings) with estimated areas ranging from 33.90–39.90 km2 with MCP (36.79–43.31% of the study area) and from 80.47–101.31 km2 with FK (109.97–104.26%). For the remaining four dolphins (14–20 sightings) the estimated areas ranged from 9.67–22.34 km2 (MCP), the predominant depth of these home ranges varied from 51–100 m (χ2 = 24.5, df = 2, P = 4.785 × 10−6). For the pods of ASD the estimated area ranged 75.23 km2 with MCP (81.66%) and 119.86 km2 with FK (130.11%) with predominant depths of 101–200 m (χ2 = 24.5, df = 2, P = 4.785 × 10−6). The area used by FIS ranged 93.27 km2 by MCP and 228.49 km2 by FK. Finally, the overlap area of BND, ASD and FIS ranged 24.75 km2 (26.86%). We point out this locality presents important oceanographic and ecological aspects which deserve to be the subject of application of management plans for the conservation of its habitat and species.

We reconstructed the late Holocene relative sea-level (RSL) evolution of the ancient harbour of Naples, one of the largest coastal conurbations in the Mediterranean. We carried out multiproxy investigations, coupling archaeological evidence with biological indicators. Our data robustly constrain 2000 yr of non-monotonic changes in sea level, chiefly controlled by the complex volcano-tectonic processes that characterize the area. Between ~200 BC and AD ~0, a subsidence rate of more than ~1.5 mm/yr enhanced the postglacial RSL rise, while negligible or moderate land uplift < ~0.5 mm/yr triggered a RSL stabilization during the Roman period (first five centuries AD). This stabilization was followed by a post-Roman enhancement of the sea-level rise when ground motion was negative, attested by a subsidence rate of ~0.5 to ~1 mm/yr. Our analysis seems to indicate very minor impacts of this nonmonotonic RSL evolution on the activities of the ancient harbour of Naples, which peaked from the third century BC to the second century AD. After this period, the progressive silting of the harbour basin made it impossible to safely navigate within the basin, leading to the progressive decline of the harbour.

Subcutaneous fat thickness and fatty acid composition (FAC) play an important role on seasoning loss and organoleptic characteristics of seasoned hams. Dry-cured ham industry prefers meats with low contents of polyunsaturated fatty acids (PUFA) because these negatively affect fat firmness and ham quality, whereas consumers require higher contents in those fatty acids (FA) for their positive effect on human health. A population of 950 Italian Large White pigs from the Italian National Sib Test Selection Programme was investigated with the aim to estimate heritabilities, genetic and phenotypic correlations of backfat FAC, Semimembranosus muscle intramuscular fat (IMF) content and other carcass traits. The pigs were reared in controlled environmental condition at the same central testing station and were slaughtered at reaching 150 kg live weight. Backfat samples were collected to analyze FAC by gas chromatography. Carcass traits showed heritability levels from 0.087 for estimated carcass lean percentage to 0.361 for hot carcass weight. Heritability values of FA classes were low-to-moderate, all in the range 0.245 for n-3 PUFA to 0.264 for monounsaturated FA (MUFA). Polyunsaturated fatty acids showed a significant genetic correlation with loin thickness (0.128), backfat thickness (−0.124 for backfat measured by Fat-O-Meat’er and −0.175 for backfat measured by calibre) and IMF (−0.102). Obviously, C18:2(n-6) shows similar genetic correlations with the same traits (0.211 with loin thickness, −0.206 with backfat measured by Fat-O-Meat’er, −0.291 with backfat measured by calibre and −0.171 with IMF). Monounsaturated FA, except with the backfat measured by calibre (0.068; P<0.01), do not show genetic correlations with carcass characteristics, whereas a negative genetic correlation was found between MUFA and saturated FA (SFA; −0.339; P<0.001). These results suggest that MUFA/SFA ratio could be increased without interfering with carcass traits. The level of genetic correlations between FA and carcass traits should be taken into account in dealing with the development of selection schemes addressed to modify carcass composition and/or backfat FAC.

The activity and circulation of influenza viruses in Argentina was studied during 2012 as part of the Argentinean Surveillance for Influenza and other Respiratory Viruses, in the context of Global Influenza Surveillance. The antigenicity and molecular characteristics of haemagglutinins (HA) of circulating influenza A and B viruses were analysed to assess the emergence of virus variants. Susceptibility to oseltamivir and zanamivir was evaluated by enzymatic assay and results were backed-up by sequencing of the neuraminidase (NA) genes. During the 2012 season, influenza virus circulation in Argentina was detected from weeks 24 to 51. The HA sequences of the studied A(H1N1)pdm09 subtype viruses segregated in a different genetic group compared to those identified during the 2009 pandemic, although they were still closely related antigenically to the vaccine virus A/California/07/2009. The HA sequences of the A(H3N2) viruses analysed fell into the A/Victoria/208/2009 clade, genetic group 3C. A mixed circulation of virus variants belonging to B/Victoria and B/Yamagata lineages was detected, with B/Victoria being dominant. All viruses tested were sensitive to oseltamivir and zanamivir except one. This isolate, an A(H1N1)pdm09 virus possessing the substitution NA-N295S, showed highly reduced inhibition by oseltamivir and reduced inhibition by zanamivir. Virological and epidemiological surveillance remains critical for detection of evolving influenza viruses.

Familial aggregation of isolated abdominal aortic and isolated intracranial aneurysms have been described. Therefore, districtual aneurysm without a systemic genetic disease (e.g. Marfan, Ehlers Danlos etc.) can have a genetic basis. The authors are reporting on the development, in the sixth decade of life, of popliteal aneurysm in two identical twins E.U. and E.C. Zygosity was established on the basis of blood-group determinations, HLA haplotypes and cytogenetic variants. Mild clinical symptoms (intermittent claudication, calf cramps) had begun in E.C. at 21 years of age. Later, at 52 years of age, a thrombosis of the aneurysmatic sac on one side required surgical treatment. E.U. showed no clinical symptoms until the age of 60, when, within the interval of a few months, bilateral thrombosis had developed. The weight difference in the two subjects (E.C. 72 Kg, E.U. 64 Kg) may explain the difference between them as regards the age of onset of symptoms and body sides (one or both) affected. Angiographic, macroscopic and histologic aspects are documented. On surgical intervention, the aneursym was found at the biforcation of the popliteal artery, but no anomalous insertion of gas-trocnemii appeared to cause pressure on the artery leading to aneurysmatic dilatation. The aneurysmatic sac measured 2.5 cm in diameter and on histological examination, using haematoxylin-eosin and Van Gieson stain, showed fragmented elastic, collagen fibres.

The coexistence of Limb body wall complex (LBWC) and Sirenomelia sequence (SS) is present in only two studies in the literature on the topic. Tang et al (1991) and Martinez-Frias et al (1992) examined single fetuses and pointed out that the association of these two phenomena is extremely rare. The present study further contributes to the investigation and description of this particular association in a pair of twins. Both twins presented abdominal defects, urogenital anomalies and persistence of the primitive cloaca, but did not show craniofacial (CF) defects or amniotic band related defects. One twin had one leg only. In a previous study on LBWC we identified two morphologically and pathogenetically different phenotypes which, among other characteristics, are identifiable by the presence or absence of CF defects. (Russo et al. 1993; Russo et al. in press). Our twin pair belongs to the LBWC group without CF defects. This group, like SS cases, is usually characterized by the presence of lower limb anomalies. Aside from this, they also share a similar pattern of anomalies involving the urogenital tract and the cloaca. Analysis of the anomalies present in the twin pair leads us to suggest that the Sirenomelia pattern might be at the extreme end in a spectrum of lower limb defects within the LBWC group that does not present CF defects. In brief, we suggest that the coexistence of this LBWC type and SS is not related to the occurrence of two different defects but is the consequence of a common maldevelopmental process occurring during blastogenesis.

Graded Al-doped ZnO layers, constituted by a mesoporous forest-like system evolving into a compact transparent conductor, were synthesized by Pulsed Laser Deposition with different morphologies to study the correlation with functional properties. Morphology was monitored by Scanning Electron Microscopy images and by measuring the resulting surface roughness. Its effects on electrical conductivity – especially carrier mobility, which significantly decreases with increasing roughness – allow to discuss the limitations in conduction mechanisms. Significant changes in light scattering capability due to variations in morphology are also investigated and discussed to study the correlation between morphology and functional properties.

Several genetic and physiological factors increase the risk of DNA damage in mammalian oocytes. Two critical events are: (i) meiosis progression, from maturation to fertilization, due to extensive chromatin remodelling during genome decondensation; and (ii) aging, which is associated with a progressive oxidative stress. In this work, we studied the transcriptional patterns of three genes, RAD51, APEX-1 and MLH1, involved in DNA repair mechanisms. The analyses were performed by real-time quantitative PCR (RT-qPCR) in immature and in vitro matured oocytes collected from 17 ± 3-month-old heifers and 94 ± 20-month-old cows. Batches of 30–50 oocytes for each group (three replicates) were collected from ovarian follicles of slaughtered animals. The oocytes were freed from cumulus cells at the time of follicle removal, or after in vitro maturation (IVM) carried out in M199 supplemented with 10% fetal calf serum, 10 IU luteinising hormone (LH)/ml, 0.1 IU follicle-stimulating hormone (FSH)/ml and 1 μg 17β-oestradiol/ml. Total RNA was extracted by Trizol method. The expression of bovine GAPDH gene was used as the internal standard, while primers for bovine RAD51, APEX-1 and MLH1 genes were designed from DNA sequences retrieved from GenBank. Results obtained indicate a clear up-regulation of RAD51, APEX-1 and MLH1 genes after IVM, ranging between two- and four-fold compared with germinal vesicle (GV) oocytes. However, only RAD51 showed a significant transcript increase between the immature oocytes collected from young or old individuals. This finding highlights RAD51 as a candidate gene marker for discriminating bovine immature oocytes in relation to the donor age.

Fourteen ‘treatment resistant’ problem gamblers received 9 weeks of Dialectical Behavior Therapy (DBT) at specialist problem gambling services delivered in Melbourne, Australia. This study is the first to investigate the effectiveness of a brief DBT treatment for problem gambling, with a focus on measuring change in the four DBT process skills (mindfulness, distress tolerance, emotion dysregulation, and negative relationships). Although there were no statistically significant improvements in measures of gambling behaviour, 83% of participants were abstinent or reduced their gambling expenditure pre- to post-treatment. Participants also reported statistically and clinically significant improvements in psychological distress, mindfulness, and distress tolerance. Moreover, there were no increases in alcohol or substance use. These results are discussed in the context of focusing on a single DBT process skill, and the benefits of using group-based approaches.