The present study documents for the first time the occurrence of amphipods in the Alacranes Reef, Gulf of Mexico. Six macrofouling assemblages were sampled from two types of man-made structures (wood docks and navigation buoys). After sorting the material, 17 species were identified. An annotated checklist for all species is provided, documenting new records for 11 species (Ampithoe marcuzzii, Apolochus cf. delacaya, Audulla chelifera, Cymadusa compta, Leucothoe cf. wuriti, Neomegamphopus hiatus, Parhyale hawaiensis, Podocerus fissipes, Quadrimaera prope pacifica, Stenothoe gallensis and Stenothoe valida).