The assessment of seed quality and physiological potential is essential in seed production and crop breeding. In the process of rapid detection of seed viability using tetrazolium (TZ) staining, it is necessary to spend a lot of labour and material resources to explore the pretreatment and staining methods of hard and solid seeds with physical barriers. This study explores the TZ staining methods of six hard seeds (Tilia miqueliana, Tilia henryana, Sassafras tzumu, Prunus subhirtella, Prunus sibirica, and Juglans mandshurica) and summarizes the TZ staining conditions required for hard seeds by combining the difference in fat content between seeds and the kinship between species, thus providing a rapid viability test method for the protection of germplasm resources of endangered plants and the optimization of seed bank construction. The TZ staining of six species of hard seeds requires a staining temperature above 35 °C and a TZ solution concentration higher than 1%. Endospermic seeds require shorter staining times than exalbuminous seeds. The higher the fat content of the seeds, the lower the required incubation temperature and TZ concentration for staining, and the longer the staining time. And the closer the relationship between the two species, the more similar their staining conditions become. The TZ staining method of similar species can be predicted according to the genetic distance between the phylogenetic trees, and the viability of new species can be detected quickly.

Graphite phase carbon nitride (g-C3N4) is a non-metal semiconductor material with a suitable band gap (2.7 eV) for visible photocatalysis. However, the high cost of relevant synthesis methods and poor adsorption performance have limited its practical applications. The objective of the present study was to mitigate these problems by synthesizing the g-C3N4 in the presence of exfoliated montmorillonite (Mnt). Compared with bulk montmorillonite, the specific surface area of exfoliated two-dimensional Mnt layers was significantly increased. As a result, the light transmittance of the lamella improved noticeably due to the fact that a freshly exposed surface had a large number of active reaction sites, making Mnt an excellent carrier for the photocatalyst g-C3N4. In order to improve the photocatalytic performance of g-C3N4, a series of g-C3N4/Mnt composites was prepared by a wet chemical method using Mnt nanolayers as the matrix. X-ray diffraction, infrared spectroscopy, Brunauer-Emmett-Teller nitrogen adsorption/desorption, transmission electron microscopy, and ultraviolet-visible diffuse reflectance spectroscopy were used to analyze the phase structure, the chemical bonds, the specific surface area and pore sizes, the morphology, and the light absorption characteristics of the composites, respectively. Rhodamine B (RhB) served as the target dye to test the photocatalytic degradation performance of the composites under visible light. According to the findings, the surface of the Mnt nanolayers was densely and uniformly covered by g-C3N4, forming a multi-layered stack structure. An increase of the calcination temperature improved the crystallinity of g-C3N4, leading first to densification and then to relaxation of the layered composite structure. Conversely, the band gap of the composite gradually decreased from 2.56 to 2.4 eV. Furthermore, temperature exposure changed the photocatalytic performance of the composite drastically. While the largest photocatalytic activity was observed at 610°C, it started to decrease with further heating of the composite. The complete degradation of RhB solution occurred after 2 h of visible light irradiation. The findings of the current study provide a scientific basis for the synthesis of a new generation of photocatalysts.

Fast neutron absorption spectroscopy is widely used in the study of nuclear structure and element analysis. However, due to the traditional neutron source pulse duration being of the order of nanoseconds, it is difficult to obtain a high-resolution absorption spectrum. Thus, we present a method of ultrahigh energy-resolution absorption spectroscopy via a high repetition rate, picosecond duration pulsed neutron source driven by a terawatt laser. The technology of single neutron count is used, which results in easily distinguishing the width of approximately 20 keV at 2 MeV and an asymmetric shape of the neutron absorption peak. The absorption spectroscopy based on a laser neutron source has one order of magnitude higher energy-resolution power than the state-of-the-art traditional neutron sources, which could be of benefit for precisely measuring nuclear structure data.

While previous research has identified the performance implications of leaders’ positive implicit followership theories (IFTs, i.e., personal expectations regarding followers’ positive characteristics), this study focuses on the effect of leader–follower congruence in positive IFTs on followers’ job performance. To test our predictions, we conducted two complementary studies. The results of Study 1 (an experiment, N = 200) show that leader–follower congruence (versus incongruence) in positive IFTs is positively related to followers’ relational identification with the leader, which, in turn, is positively related to followers’ job performance. Moreover, followers’ uncertainty avoidance strengthens this relationship. These findings were replicated in Study 2 (a three-wave survey, N = 223) through polynomial regression and response surface analysis. This study improves our understanding of IFTs by showing that leader–follower congruence in this domain is related to followers’ outcomes.

Flexible cables in cable-driven parallel robots (CDPRs) are easy to be excited and vibrate. Cable vibration will react on the end-effector, causing attitude deviation of the end-effector. The main objective of this study is to accurately model axially moving flexible cables and characterize the dynamic behaviors of associated compliant CDPRs. Firstly, a model for transverse vibration of the axially moving length-variable cable is developed. On this basis, an original nonlinear dynamic model of the CDPRs able to capture the vibration of the cables and the dynamics of the end-effector is proposed. Secondly, the frequency–amplitude relationship of the CDPR is obtained. Moreover, the significance of the excitation effect caused by the axially moving length-variable cables is demonstrated, by comparing the results with and without excitation effect at different frequencies. It turns out that, as the oscillation frequency of the end-effector increases, the end-effector and cables exhibit the dynamics process from steady state to unstable large-amplitude vibration and finally to stable small-amplitude vibration. This indicates that the dynamics of the CDPR exhibit non-linear characteristics, due to the influence of flexible cables. Finally, the proposed dynamic model of compliant CDPRs is validated by experiments performed in the laboratory.

The phenomenon that prior gains may increase people’s willingness toaccept risky gambles is named as the house money effect (Thalerand Johnson, 1990). Many studies have shown that the “house moneyeffect” is a robust phenomenon but few scholars explain the mechanism ofit well. We suppose the reason for the house money effect is that the ante(starting amount) is from the prior gambling profits, and its potential loss hasrelatively low psychological value. To test this hypothesis, we designed aseries of studies using two-stage gambles. A total of 915 university studentsparticipated. In Study 1, in addition to a standard condition (which replicatedthe basic effect), we test how people respond to “prospect theory, withmemory” frame, a “concreteness” frame and“quasi-hedonic” editing. None of these types of frames result in asignificant house money effect. In Study 2, we certify the reference point shiftto 100 Yuan in the second-stage gamble, thus the house money effect can beregarded as the absence of loss aversion; Study 3, consisting of 3sub-experiments, indicated that gambling profits and normal income will opendifferent mental accounts which are spent quite differently. The pain of losing100 Yuan allowance is more serious than that of losing 100 Yuan gambling wins.People will typically reject the gamble of 50/50 chance to gain or lose 100 Yuanif the ante is from the “normal income account”, but accept if theante is from the “windfall account”. The results of the series ofexperiments prove the accuracy of our hypothesis mostly.

This research commentary responds to Jiang and Murmann (2022), by offering further explanations for the miraculous growth of the digital economy in China from both the demand and supply perspectives. To complement their views, we identify several additional important factors, and classify them in three categories in an explanatory model. We believe that factors on the demand side played a primary role, along with innovative and entrepreneurial e-commerce service providers on the supply side. Infrastructures and government policies provided the foundation and a facilitating environment. We conclude that digital transformation of traditional industries will become the next wave of digital economy, stronger than the previous wave of e-commerce. Implications for research and practices are also discussed. In addition, we highlight emerging trends and turbulences. In conclusion, despite short-term turbulences, we expect that the digital economy in China will make big-stride progress in the long term, with strong potential to continue its growth.

Hepatitis B virus-related acute-on-chronic liver failure (HBV-ACLF) is a severe and life-threatening complication, characterised by multi-organ failure and high short-term mortality. However, there is limited information on the impact of various comorbidities on HBV-ACLF in a large population. This study aimed to investigate the relationship between comorbidities, complications and mortality. In this retrospective observational study, we identified 2166 cases of HBV-ACLF hospitalised from January 2010 to March 2018. Demographic data from the patients, medical history, treatment, laboratory indices, comorbidities and complications were collected. The mortality rate in our study group was 47.37%. Type 2 diabetes mellitus was the most common comorbidity, followed by alcoholic liver disease. Spontaneous bacterial peritonitis, pneumonia and hepatic encephalopathy (HE) were common in these patients. Diabetes mellitus and hyperthyroidism are risk factors for death within 90 days, together with gastrointestinal bleeding and HE at admission, HE and hepatorenal syndrome during hospitalisation. Knowledge of risk factors can help identify HBV-ACLF patients with a poor prognosis for HBV-ACLF with comorbidities and complications.

We investigate the effect of the secondary baroclinic vorticity (SBV) on the energy cascade in the mixing induced by the multi-mode Richtmyer–Meshkov instability (RMI). With the aid of vorticity-based simplified models and the vortex-surface field, we find that the effect of the SBV peaks at a critical time when the vortex reconnection widely occurs in the mixing zone. Before the critical time, spikes and bubbles evolve almost independently, and we demonstrate that the variation of the kinetic energy spectrum induced by the SBV has the $-1$ scaling law at intermediate wavenumbers using the model of vortex rings. This SBV effect causes the slope of the total energy spectrum at intermediate wavenumbers to evolve towards $-3/2$ at the critical time. Subsequently, the SBV effect diminishes and the energy spectrum decays to the $-5/3$ law. Inspired by the vortex dynamics, we develop a model for estimating the mixing width and validate the model using numerical simulations of the multi-mode RMI with various modes of initial perturbations. This model captures the nonlinear growth of the mixing width before the self-similar growth stage.

Drilling and sampling are the most direct and effective methods available to study Antarctic subglacial lakes. Based on the Philberth probe, a Recoverable Autonomous Sonde (RECAS) allows for in situ lake water measurement and sampling, through the addition of an upper thermal tip and a cable recoiling mechanism. RECAS-200, a prototype of RECAS, has a drilling depth of 200 m, a surface supply voltage of 800 VAC and a downhole power of ~9.6 kW during drilling. In this study, a heating control system for RECAS-200 was designed. The system avoids the need for high-power step-down converters, by separating heating power from control power, thereby reducing the overall weight of the probe and avoiding the need to increase cable diameter. We also introduce a self-developed, small, solid-state, 800 VAC power regulator and a fuzzy PID temperature control algorithm. Their purpose was to manage the power adjustment of each heating element and to provide closed-loop temperature control of certain heating elements which can easily burn out due to overheating. Test results indicated that the proposed RECAS-200 heating control system met all our design specifications and could be easily assembled into the RECAS-200 probe.

Subglacial lake exploration is of great interest to the science community. RECoverable Autonomous Sonde (RECAS) provides an exploration tool to measure and sample subglacial lake environments while the subglacial lake remains isolated from the glacier surface and atmosphere. This paper presents an electronic control system design of 200 m prototype of RECAS. The proposed electronic control system consists of a surface system, a downhole control system, and a power transfer and communication system. The downhole control system is the core element of RECAS, and is responsible for sonde status monitoring, sonde motion control, subglacial water sampling and in situ analysis. A custom RS485 temperature sensor was developed to cater for the limited size and depth requirements of the system. We adopted a humidity-based measurement to monitor for a housing leak. This condition is because standard leak detection monitoring of water conductivity may be inapplicable to pure ice in Antarctica. A water sampler control board was designed to control the samplers and monitor the on/off state. A high-definition camera system with built-in storage and self-heating ability was designed to perform the video recording in the subglacial lake. The proposed electronic control system is proven effective after a series of tests.

We report an experimental study of the Prandtl-number effects in quasi-two-dimensional (quasi-2-D) Rayleigh–Bénard convection. The experiments were conducted in four rectangular convection cells over the Prandtl-number range of $11.7 \leqslant Pr \leqslant 650.7$ and over the Rayleigh-number range of $6.0\times 10^8 \leqslant Ra \leqslant 3.0\times 10^{10}$. Flow visualization reveals that, as $Pr$ increases from 11.7 to 145.7, thermal plumes pass through the central region much less frequently and their self-organized large-scale motion is more confined along the periphery of the convection cell. The large-scale flow is found to break down for higher $Pr$, resulting in a regime transition in the Reynolds number $Re$. For the $Pr$ range with a large-scale flow of system size, the $Re$ number, Nusselt number $Nu$ and local temperature fluctuations were investigated systematically. It is found that $Re$ scales as $Re \sim Ra^{0.58}Pr^{-0.82}$ in the present geometry, which suggests that it is in line with the behaviour in the 2-D configuration. On the other hand, the measured $Nu(Ra, Pr)$ relation $Nu \sim Ra^{0.289}Pr^{-0.02}$ tends to be compatible with the finding in a three-dimensional (3-D) system. For the temperature fluctuations in the cell centre and near the sidewall, they exhibit distinct $Ra$-dependent scalings that could not be accounted for with existing theories, but their $Pr$ dependences for $Pr \lesssim 50$ are in agreement with the predictions by Grossmann & Lohse (Phys. Fluids, vol. 16, 2004, pp. 4462–4472). These results enrich our understanding of quasi-2-D thermal convection, and its similarities and differences compared to 2-D and 3-D systems.

We elucidate the effect of the secondary baroclinic vorticity (SBV) on the Richtmyer–Meshkov instability (RMI) accelerated by a weak incident shock and develop a vortex-based model for spike and bubble growth rates. Two major mechanisms of the single-mode RMI, the primary baroclinic vorticity (PBV) and the pressure perturbation, are distinguished by simplified models with the vortex-surface field. We find that the effect of the pressure perturbation can be neglected in the present RMI, and the growth of the interface or vortex surface is first driven by the PBV. Subsequently, the SBV, generated by the misalignment between the density gradient across the interface and the pressure gradient produced by the PBV-induced velocity, leads to the nonlinear growth of the interface with the generation of spikes and bubbles. Inspired by this mechanism, we develop a predictive model of spike and bubble growth rates using the motion of viscous vortex rings. The circulation of the vortex ring is modelled with the SBV effect. This model is validated by five data sets of direct numerical simulations and experiments of the single-mode RMI with various initial conditions.

Much progress has been made in understanding the environmental and hormonal systems regulating winter diapause. However, transcriptional regulation of summer diapause is still largely unknown, making it difficult to understand an all-around regulation profile of seasonal adaptation. To bridge this gap, comparison RNA-seq to profile the transcriptome and to examine differential gene expression profiles between non-diapause, summer diapause, and winter diapause groups were performed. A total number of 113 million reads were generated and assembled into 79,117 unigenes, with 37,492 unigenes categorized into 58 functional gene ontology groups, 25 clusters of orthologous group categories, and 256 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. KEGG analysis mapped 2108 differentially expressed genes to 48 and 67 pathways for summer and winter diapauses, respectively. Enrichment statistics showed that 11 identical pathways similarly overlapped in the top 20 enriched functional groups both related to summer and winter diapauses. We also identified 35 key candidate genes for universal and differential functions related to summer and winter diapause preparation. Furthermore, we identified some genes involved in the signaling and metabolic pathways that may be the key drivers to integrate environmental signals into the summer and winter diapause preparation. The current study provided valuable insights into global molecular mechanisms underpinning diapause preparation.

Drug-induced liver injury (DILI) is a common adverse drug reaction leading to the interruption of tuberculosis (TB) therapy. We aimed to identify whether the hepatitis B virus (HBV) infection would increase the risk of DILI during first-line TB treatment. A meta-analysis of cohort studies searched in PubMed, Web of Science and China National Knowledge Infrastructure was conducted. Effect sizes were reported as risk ratios (RRs) and 95% confidence intervals (CIs) and calculated by R software. Sixteen studies with 3960 TB patients were eligible for analysis. The risk of DILI appeared to be higher in TB patients co-infected with HBV (RR 2.66; 95% CI 2.13–3.32) than those without HBV infection. Moreover, patients with positive hepatitis B e antigen (HBeAg) were more likely to develop DILI (RR 3.42; 95% CI 1.95–5.98) compared to those with negative HBeAg (RR 2.30; 95% CI 1.66–3.18). Co-infection with HBV was not associated with a higher rate of anti-TB DILI in latent TB patients (RR 4.48; 95% CI 0.80–24.99). The effect of HBV infection on aggravating anti-TB DILI was independent of study participants, whether they were newly diagnosed with TB or not. Besides, TB and HBV co-infection patients had a longer duration of recovery from DILI compared to non-co-infected patients (SMD 2.26; 95% CI 1.87–2.66). To conclude, the results demonstrate that HBV infection would increase the risk of DILI during TB therapy, especially in patients with positive HBeAg, and close liver function monitoring is needed for TB and HBV co-infection patients.

For the guarantee of the long-distance transport of the bunches of China Initiative Accelerator Driven System (CIADS), a new scheme is proposed that extra magnetic field is used in the accelerator-target coupling section before the windowless target to minimize the self-modulation (SM) mechanism. Particle-in-cell simulations are carried out to study the influence of the solenoidal magnetic field on the self-modulation mechanism when long proton bunches move in the background plasmas. The long proton bunches used in the simulations are similar to these in the linear accelerator of CIADS. It is found that the presence of the solenoidal magnetic field will significantly inhibit the self-modulation process. For the strong magnetic field, the longitudinal separation and transverse focusing of the long bunches disappear. We attribute these phenomena to the reason that the strong solenoidal magnetic field restricts the transverse movement of plasma electrons. Thus, there are not enough electrons around the bunch to compensate the space charge effect. Moreover, without transverse current, the longitudinal pinched effect disappears, and the long bunch can not be separated into small pulses anymore.