Nine characters contributing to seed yield were measured on 20 accessions of horse gram (Macrotyloma uniflorum (Lam.) Verd), and subjected to genetic divergence analysis using Mahalanobis statistic and mapping using DIVA-GIS. The accessions were collected from two eco-geographical regions of Andhra Pradesh (India) – North Coastal and Rayalaseema. Based on D2 values the genotypes were grouped into five clusters. Genetic diversity was not related to eco-geographical distribution. The greatest inter-cluster distance separated clusters II and V, followed by clusters IV, and V, III and IV. Entries in clusters V and II appear suitable as parents for horse gram improvement. The Rayalaseema region is the source of useful variation for days to flowering, maturity and yield.