The recent introduction of glufosinate-resistant and glyphosate-resistant crops provides growers with new options for weed management. Information is needed to compare the effectiveness of glufosinate and glyphosate on annual weeds. Greenhouse trials were conducted to determine the response of barnyardgrass (Echinochloa crus-galli), common lambsquarters (Chenopodium album), common ragweed (Ambrosia artemisiifolia), fall panicum (Panicum dichotomiflorum), giant foxtail (Setaria faberi), large crabgrass (Digitaria sanguinalis), and velvetleaf (Abutilon theophrasti) to glufosinate and glyphosate. The response of velvetleaf and common lambsquarters was investigated at multiple stages of growth. Glufosinate and glyphosate were applied to each weed species at logarithmically incremented rates. The glufosinate and glyphosate rates that provided a 50% reduction in aboveground weed biomass, commonly referred to as GR50 values, were compared using nonlinear regression techniques. Barnyardgrass, common ragweed, fall panicum, giant foxtail, and large crabgrass responded similarly to glufosinate and glyphosate. Common lambsquarters 4 to 8 cm in height was more sensitive to glufosinate than glyphosate. In contrast, 15- to 20-cm tall-velvetleaf was more sensitive to glyphosate than glufosinate.