Kappa (k-), iota (ɩ-), and lambda (λ-) Carrageenan (Car) microparticles were synthesized by crosslinking corresponding natural polymers with divinyl sulfone (DVS) via microemulsion polymerization/crosslinking methods for potential biomedical applications. Negatively charged e.g., -44 mV of Car particles were modified with diethylenetriamine (DETA) to generate positively charged modified (M-) ᴋ-Car particles with +23 mV zeta potential values. Cationic M-Car-DETA particles were found to be effectively antimicrobial material against gram negative bacteria, and some fungi species. Moreover, upon protonation of M-Car-DETA particles with HCl the effectiveness against all types of microorganisms are significantly increased. All types of Car based particles can be applicable for blood contacting material as low hemolysis ratio, 2.56% and high blood clotting capability, 86% were obtained. Furthermore, Car based particles found biocompatible against L929 fibroblast cells, and can induce necrotic cell death at 12.5 μg/mL concentration against DLD-1 colon cancer cells. M-ᴋ-Car-DETA particles were also demonstrated to be useful rending long term and linear drug release profiles using Rosmarinic Acid (RA) as a model drug.