Dante Cicchetti, the architect of developmental psychopathology, has influenced so many of us in profound ways. One of his many contributions was in demonstrating the power of randomized controlled trials (RCTs) to study the effects of Child–Parent Psychotherapy (CPP). These RCTs have shed light on causal mechanisms in development. Following Cicchetti and colleagues’ work, we designed a brief home visiting program, Attachment and Biobehavioral Catch-up (ABC), to help parents respond in sensitive, nurturing ways, so as to enhance children’s attachment and self-regulatory capabilities. In the current study, we assessed adolescents’ reports of the closeness of their relationships with their mothers 12 years after their mothers completed the intervention. A total of 142 adolescents participated (47 randomized to ABC, 45 randomized to a control intervention, and 50 from a low-risk comparison group). Adolescents whose mothers had been randomized to ABC reported closer relationships with their mothers than adolescents randomized to the control condition, with significant differences seen on approval, support, companionship, and emotional support subscales. Consistent with Cicchetti et al.’s work, these results provide powerful evidence of the long-term effects of an early parenting intervention.

We describe an ultra-wide-bandwidth, low-frequency receiver recently installed on the Parkes radio telescope. The receiver system provides continuous frequency coverage from 704 to 4032 MHz. For much of the band ( ${\sim}60\%$ ), the system temperature is approximately 22 K and the receiver system remains in a linear regime even in the presence of strong mobile phone transmissions. We discuss the scientific and technical aspects of the new receiver, including its astronomical objectives, as well as the feed, receiver, digitiser, and signal processor design. We describe the pipeline routines that form the archive-ready data products and how those data files can be accessed from the archives. The system performance is quantified, including the system noise and linearity, beam shape, antenna efficiency, polarisation calibration, and timing stability.

Barramundi (Lates calcarifer) are a highly valued aquaculture species, and, as obligate carnivores, they have a demonstrated preference for dietary protein over lipid or starch to fuel energetic growth demands. In order to investigate how carnivorous fish regulate nutritional cues, we examined the metabolic effects of feeding two isoenergetic diets that contained different proportions of digestible protein or starch energy. Fish fed a high proportion of dietary starch energy had a higher proportion of liver SFA, but showed no change in plasma glucose levels, and few changes in the expression of genes regulating key hepatic metabolic pathways. Decreased activation of the mammalian target of rapamycin growth signalling cascade was consistent with decreased growth performance values. The fractional synthetic rate (lipogenesis), measured by TAG 2H-enrichment using 2H NMR, was significantly higher in barramundi fed with the starch diet compared with the protein diet (0·6 (se 0·1) v. 0·4 (se 0·1) % per d, respectively). Hepatic TAG-bound glycerol synthetic rates were much higher than other closely related fish such as sea bass, but were not significantly different (starch, 2·8 (se 0·3) v. protein, 3·4 (se 0·3) % per d), highlighting the role of glycerol as a metabolic intermediary and high TAG-FA cycling in barramundi. Overall, dietary starch significantly increased hepatic TAG through increased lipogenesis. Compared with other fish, barramundi possess a unique mechanism to metabolise dietary carbohydrates and this knowledge may define ways to improve performance of advanced formulated feeds.

Background: In 2016 The Fraser Health Authority's Emergency Network established a priority to standardize patient access and flow through their 13 emergency departments (ED). A Model of Care (MOC) was developed after an extensive review of the literature and current practices across BC. Aim Statement: The ED Model of Care (MOC) specifies best practice expectations with respect to emergency patient access and flow. Rather than a ‘top-down’ mandate of expected practices, the MOC provided the opportunity for site-based teams to promote solutions that were ‘locally actioned and regionally enabled’. Measures & Design: ED Quality Improvement (QI) teams were developed at all sites. The ED Network developed a “QI Bootcamp”, a one-day course focused on imparting tools to drive improvements, providing a baseline understanding of how to launch and sustain local QI initiatives. Using Prosci's change approach, an emphasis was placed on using local ingenuity to implement plans, analyze feedback and diagnose gaps. This approach measured utilization of the changes to tangibly link initiatives and change to specific outcomes. As part of this strategy, an online scorecard was created to measure local results against best practice outcomes. The scorecard tracked quantitative access metrics such as ED Length of Stay (EDLOS), Left Without Being Seen rate, and triage time. Measures such as forming a QI team, identifying a QI project and completing a PDSA cycle were included in the scorecard Evaluation/Results: The MOC change management strategy was launched in May of 2018. By December 2018 all 13 EDs had formed a local QI team and identified a project. Twelve sites had completed at least one PDSA cycle and 10 sites had at least 75% of their members attend the QI Bootcamp. The scorecard displayed improvements in flow metrics. Highlights include the average arrival to triage time decreasing by 36% at one site, EDLOS for moderately ill patients decreased from 4.8 to 3.4 hours at another, and a community hospital had low acuity patient EDLOS decrease from 3.52 to 2.37 hours. Discussion/Impact: A standardized approach to patient access and flow in the ED (MOC), combined with the engaging grass roots approach to inspiring local innovation, allied with a concrete change management approach demonstrated significant results for patients accessing and moving through EDs. This pattern that is more likely to sustain itself because the results are felt and locally owned.

The autosomal genes mitochondrial transcription factor A (TFAM) and uncoupling protein 2 (UCP2) are both involved in moderating mitochondrial energy production. This study investigated single nucleotide polymorphisms (SNPs) in TFAM and UCP2 and their associations with growth, fertility, milk production and survival in UK dairy cows. Holstein–Friesian heifers (n = 509) were recruited at birth and monitored until they either reached the end of their second lactation or were culled. Weight, height, length and girth were measured at 1, 6 and 15 months of age to assess growth. Fertility records were obtained for both heifers and cows and production traits (milk per day, peak yield and 305-day yield) were recorded in the first and second lactation. Mixed model and survival analyses revealed that TFAM3 GG homozygotes, representing 49% of the population, were larger than the AG heterozygotes throughout the growing period, had worse fertility, produced less milk in both lactations and were more likely to be culled. TFAM3 AA homozygotes (7% of population) were generally smaller as heifers, had slightly worse fertility and also produced less milk. This suggests a clear advantage for maximizing the heterozygotic population for this SNP. Furthermore, the results for milk per day suggest an overdominant effect, as both homozygotes of TFAM3 had significantly lower yields than the heterozygote. For UCP2, the minority CG heterozygotes (6% of population) were larger as heifers, had a reduced age at first conception and a delayed return to cyclicity after calving, when compared against the majority (94%) GG homozygotes.

Tick populations were observed on zebu cattle (Bos indicus) over an 18 month period at Patiko in northern Uganda. Monthly collections were made of all stages of ticks from five cattle and six sheep (Ovis aries). Collections of adults of Rhipicephalus appendiculatus Neumann were also made from the ears of about 30 cattle on several occasions. The species recorded on cattle, in order of decreasing abundance, were R. appendiculatus, Amblyomma variegatum (Fabricius), Boophilus decoloratus (Koch) and R. evertsi evertsi Neumann. Far fewer ticks were collected from the sheep. The adults of R. appendiculatus were randomly distributed over the herd. There was a distinct seasonal incidence of R. appendiculatus and A. variegatum on their hosts, which could be related to rainfall and to the emergence of new cohorts during the long rainy season. In addition, adult females of R. appendiculatus were collected daily off the ears of cattle at Entebbe following an infestation with 200 adult males and females. Measurements of the growth of the ticks were used to define the sizes of ‘standard’ ticks which would complete engorgement in the following 24 h. Such ticks provide a means of sampling to estimate the number of ticks engorging daily. Comparison of the frequency distributions of each stage and species in the Patiko collections were then used to infer the sizes of standard ticks for other species.

A DNA probe PCsv4 and a subclone thereof PCsv4.1, hybridize specifically to rodent malaria DNA. DNA purified from a small volume (10 μ1) of infected mouse blood was used to determine the composition of the parasite population present. The hybridization signal following PCsv4 probing of slot-blotted DNA correlated directly with parasitaemia. The hybridization pattern and Intensity, resulting from probing restriction enzyme digested and Southern-blotted genomic DNA, determined the identity of the infecting parasite line(s), and provided a semi-quantitative measure of parasite burden. Fifteen parasite lines representative of all four Plasmodium species infecting rodents can be differentiated in this way.

Cloned lines of the four rodent Plasmodium species can be differentiated by the RFLP pattern generated following Southern blotting and probing with PCsv4.1, a probe derived from a P. chabaudi chabaudi genomic library. Groups of CBA/Ca mice were inoculated simultaneously with cloned lines from two parasite species or strains. Six mixed species and three mixed strain infections using rodent malaria lines were initiated. The composition of the parasite population in each group was determined qualitatively and semi-quantitatively by analysis of the DNA purified from daily blood samples, thereby providing a dynamic representation of each mixed infection. Effects on the course of parasitaemias are presented and discussed.

The average dairy cow survives only three lactations, reducing the availability of replacement heifers. Prenatal losses occur due to early embryonic mortality (about 40%), later embryo loss (up to 20% in high-yielding herds) or abortion (about 5%). A recent survey of 19 UK herds showed that 7.9% of calves were born dead and 3.4% died within 1 month. During the rearing phase, 6.7% of animals were lost before reaching first service at 15 months due to disease or accident and another 2.3% failed to conceive. Many potential replacements therefore never enter the milking herd. This severely limits opportunities for on-farm selection of breeding cows in addition to presenting a welfare issue and causing economic loss. The most profitable animals once lactation is reached combine good milk production with a regular calving pattern. Some aspects of performance are related to age at first calving (AFC), which in turn is influenced by heifer growth rates. Poorly growing animals required more services to conceive, calved later and subsequently performed badly. Optimum fertility and maximum yield in the first lactation were associated with an AFC of 24 to 25 months. However, heifers calving at 22 to 23 months performed best in terms of total milk yield and survival over the first 5 years, partly because good heifer fertility was associated with better fertility later. We have investigated some possible juvenile predictors of future performance. Low-birth-weight calves were more likely to come from either primiparous mothers or older dams (3+ lactations) with higher peak milk yields, suggesting that the uterine environment may limit prenatal calf growth due to competition for nutrients with maternal growth or milk production. Linear trait classification scores for frame size show genetic correlations with longevity. The skeletal measures of height and crown rump length in 1-month-old calves was correlated to subsequent stature, and frame size was correlated to weight at 15 months. It may thus be possible to predict performance from simple size measurements as juveniles. Neither endogenous nor stimulated growth hormone (GH) release in 6-month-old calves were related to milk yield in the first three lactations, but size of a stimulated GH peak was positively related to milk energy values in the first lactation. Cows with delayed ovulation (>45 days) in the first lactation had a higher GH pulse amplitude and lower IGF-I as a juvenile. Cows that partition excess energy into milk in their first lactation may suffer reduced longevity.

A short herd lifespan is a significant economic loss to the dairy industry, with infertility a major cause. Conception rates to first service have fallen from around 60% in the 1970s to 40% by 2000. The re-establishment of ovarian cyclicity after calving is important in determining the timing of conception which should be around 80 days postpartum (PP) to maintain yearly calving intervals. Insulin-like growth factor-I (IGF-I) is thought to influence ovarian activity at this time. We have previously shown that pre-pubertal IGF-I levels are related to growth and can predict concentrations around first calving. This study investigated IGF-I, insulin, glucose, urea and betahydroxybutyrate (BHB) concentrations before and after first calving in typical UK herds in relation to measures of fertility during the first lactation.

Reduced reproductive performance of dairy cows is a major challenge facing the dairy industry. Many potential replacement heifers fail to reach their first lactation because they are either born dead, die as juveniles, or fail to conceive. Perinatal mortality has previously been estimated across English herds as 7.8% (Esslemont & Kossaibati, 1996), whereas neonatal mortality in the UK has been estimated at 0 to 10% (reviewed by Mellor & Stafford, 2004). Calf mortality is often poorly documented and estimates that are available are possibly underestimated because a dead calf at birth is not always recorded at the farm level. Therefore, the objective of this study was to accurately calculate the incidence of perinatal and neonatal mortality of dairy calves on commercial farms in southern England.

The decline in dairy cow fertility over the past 30 years has major economic, welfare, genetic and environmental consequences. A significant number of potential replacement heifers either never calve or else complete only a single lactation. The relationships between metabolic status and fertility may change with age as cows reach physical maturity. To determine the reasons behind the high loss rates attributable to infertility, this study tracked a group of animals until the end of their second lactation to compare fertility and metabolic hormone profiles in the same cows at different ages.

A two-dimensional method was used to observe the interactions of plane shock waves with single cavities. This allowed study of processes occurring within the cavity during collapse. Results were obtained from high-speed framing photography. A variety of collapse shock pressures were launched into thin liquid sheets either by firing a rectangular projectile or by using an explosive plane-wave generator. The range of these shock pressures was from 0.3 to 3.5 GPa. Cavities were found to collapse asymmetrically to produce a high-speed liquid jet which was of approximately constant velocity at low shock pressures. At high pressures, the jet was found to accelerate and crossed the cavity faster than the collapse-shock traversed the same distance in the liquid. In the final moments of collapse, high temperatures were concentrated in two lobes of trapped gas and light emission was observed from these regions. Other cavity shapes were studied and in the case of cavities with flat rear walls, multiple jets were observed to form during the collapse.

An experimental study of the interaction of weak shock waves in a liquid with bubbles and solid particles has been conducted. Cavities were punched, and solid particles were cast, into a thin sheet of gelatine clamped between two transparent blocks. A shock of pressure 0.3 GPa was introduced by impacting the gelatine layer with a flyer plate. The subsequent collapse of the cavities was photographed using high-speed framing cameras, and waves in the gelatine were visualized using schlieren optics. Assorted cavity/particle geometries were studied. In the first, cavity and particle were aligned on an axis parallel to the incident shock front. The jet crossing the cavity was found to deviate from the perpendicular to the shock front. This deviation was towards the solid particle when separations were small and away from the particle when separations were increased. When a cavity was placed upstream of a solid particle the collapse time was reduced. Conversely, when a cavity was placed downstream of a solid particle, collapse time was increased and the closure was more symmetrical. These observations were explained in terms of wave reflections. Collapses where the cavity/particle axis was inclined to the incident shock showed features of each of the geometries described above.