The variations in the composition and structure of macroinvertebrate benthic communities in relationship with the marine sediment enrichment with heavy metals were investigated in the Gulf of Gabes, Tunisia. Standard community parameters as well as the trophic and ecological structure were analysed in 18 stations sampled in six localities. Highest values of diversity descriptors (S, N and H′) were recorded in the less-polluted localities and vice versa. Besides, the results of AMBI and BENTIX indexes were also concordant with those obtained with the classical diversity parameters and matched with the sediment heavy metals distribution in the Gulf of Gabes. Compared with the northern and southern parts of the Gulf, the central area was found to be the most polluted and to host the most-affected benthic community. In addition, biotic indexes were found to be very useful tools to monitor the ecological quality status of benthic assemblages.