Introduction. The fruit types such as raspberries (Rubus
idaeus), cherries (Prunus cerasus), blackberries
(Rubus fructicosus), blackthorns (Prunus spinosa) and
aronia (Aronia melanocarpa) are very common in Serbia. These fruit
species are a valuable source of antioxidants. The goal of our work was to evaluate the
antioxidant activities of ethyl acetate extracts of these five berries and to establish
possible correlation between the content of anthocyanin and the antioxidant activity.
Materials and methods. To determine antioxidant activity of the selected
fruits, the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity, the ABTS
(2,2-azinobis-3 ethyl benzothiazoline-6-sulfonic acid) cation decolorization activity, the
FRAP (ferric reducing antioxidant power), and the TRP (total reducing power) were
measured. Results. The four methods selected for our assays showed strong
antioxidant properties for blackberry samples. The total anthocyanin content was estimated
using the pH differential method. The highest amount of anthocyanin was found in
blackberry samples [(1063.53 ± 0.01) mg·kg–1 fresh fruit], while the lowest was in
raspberry samples [(180.84 ± 0.02) mg·kg–1 fresh fruit]. The correlation between the
content of anthocyanin and antioxidant activity was established using regression analysis.
The highest correlation was found between total reducing power and total anthocyanin
(r = 0.97, p < 0.05). Hierarchical cluster analysis
divided selected fruit species into two statistically significant clusters.
Conclusion. Our results confirmed that analyzed berries are rich in
anthocyanins. A strong correlation among different assays as well as with anthocyanin
content was observed. Cluster analysis can be used in food science, to classify different
food types into groups, based on similarity among the results.