The retention of salts in laboratory-grown ice was compared to the retention of extracellular polysaccharide substances (EPS) produced by the cold-adapted marine gammaproteobacterium, Colwellia psychrerythraea strain 34H. Saline ice was formed, by means of a cold-finger apparatus, from artificial sea-water solutions containing either native dissolved EPS from strain 34H, the same EPS but heat-treated, or dissolved EPS from the uninoculated growth medium. Results indicated that only the native (unheated) EPS of strain 34H was retained preferentially in the ice. Temperature and volumetric measurements of the ice further suggested a link between the heat-labile fraction of this EPS of marine bacterial origin and potential habitat alteration. Bacterial EPS may join algal EPS in our understanding of how extracellular polymers help to establish and sustain the microbial community that inhabits sea ice.