Based on Pettigrew and Meertens’ Blatant and Subtle Prejudice Scale (1995), a new scale for assessing the expression of prejudice is proposed in this article. The original scale has been changed in three ways: (a) items were rewritten to avoid double assertions, (b) three new items were considered to adequately measure the ‘denial of positive emotions’ component, and (c) biased wording of the ‘cultural differences’ domain items measuring perceived cultural differences rather than prejudices was removed. We used a representative sample of 500 participants living in Antofagasta, in Chile. Confirmatory factor and a hierarchical regression analyses support the proposed new scale. This instrument provides two measures: one general measure related to the intensity of prejudice expression and a second measure indicating the presence or absence of prejudice for each component type (Rejection, Intimacy, Values, Emotions, and Culture). The scale was confirmed for both genders; 45.2% of the participants scored a medium-high to extremely high expression of prejudice towards Bolivians immigrants.