This study was carried out on fatty acid composition in wild and cultured varieties of two fish species, Epinephelus coioides and Sparidentex hasta. Fresh fish samples were collected from Hormozgan, Iran. After lipid extraction, fatty acids composition was analysed by gas chromatography. Results showed DHA (C22:6n3), EPA (C20:5n3), palmitic acid (C16:0), stearic acid (C18:0), myristic acid (C14:0), oleic acid (C18:1n9c) and palmitoleic acid (C16:1) were important fatty acids in the fish samples. There was low concentrate of linolenic acid (ALA, C18:3n3) in all analysed samples. Cultured fishes were found to contain significantly higher values of SFA, while their wild relative contained higher levels of MUFA followed by PUFA. In the wild fish species among PUFA, DHA were the most abundant followed by EPA. Altogether, EPA was the most abundant followed by DHA in the cultured fish species. Also the ω3/ω6 ratio was significantly higher in wild fishes and it was highest (13.04) in wild E. coioides.