Immobilization of DNA/RNA, onto various metal and metal oxide surfaces is of great importance for the development of future microarray, gene mapping, DNA sequencing, nanoparticle targeting, and sensor applications. Attachment of DNA to solid interfaces typically occurs through either electrostatic interactions or covalent bonds to functional groups introduced to nucleic acid termini. Previously, we and others have demonstrated that alkanephosphates and terminal phosphate groups present on nucleic acids play an important role in the interaction with group IV metal oxides such as zirconium and hafnium, providing a stable linkage to the surface. Titanium dioxide (TiO2), which is frequently employed in various nanoscale applications, belongs to the same group and similar interactions with phosphate are expected. Various adsorption studies have demonstrated binding of nucleic acids to TiO2 surfaces, although the influence of terminal phosphate versus electrostatic interaction (via the DNA/RNA backbone) on the surface interaction is unclear. The research presented here investigates the effect of nucleic acid length, presence of terminal phosphates, and differences between dsDNA and ssDNA on their binding to TiO2 nanoparticles. TiO2 nanoparticles (20 nm) were used to study the adsorption of Lambda DNA (˜48 kbp), and shorter (21 bp) ssDNA and dsDNA oligonucleotides with and without a 5’ phosphate group. Initial adsorption of DNA to nanoparticles was calculated via UV absorption. Results showed that all types of nucleic acids (Lamda DNA, ssDNA and dsDNA) initially bind to nanoparticles, independent of molecular weight single/double strandedness, or phosphorylation state. The total amount of DNA initially adsorbed to nanoparticles (ng/particle) differs between ssDNA and dsDNA, as well as the length of the DNA used. These results show that nucleic acid interactions with TiO2 nanoparticles are not dependent upon the presence of a terminal phosphate group. These results provide valuable data for future applications based on DNA-nanoparticle constructs including nanoelectronics, photovoltaics, and biotemplated synthesis of semiconducting materials.