To save content items to your account,
please confirm that you agree to abide by our usage policies.
If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account.
Find out more about saving content to .
To save content items to your Kindle, first ensure firstname.lastname@example.org
is added to your Approved Personal Document E-mail List under your Personal Document Settings
on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part
of your Kindle email address below.
Find out more about saving to your Kindle.
Note you can select to save to either the @free.kindle.com or @kindle.com variations.
‘@free.kindle.com’ emails are free but can only be saved to your device when it is connected to wi-fi.
‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.
Bacteriophage φ6 has a double-stranded RNA
genome composed of three linear segments, L, M, and S.
The innermost particle in the virion of φ6, like in
the other dsRNA viruses, is an RNA-dependent RNA polymerase
complex, which carries out all the functions needed for
the replication of the viral genome. Empty polymerase complexes
can package the single-stranded copies of the viral genome
segments, replicate the packaged segments into double-stranded
form (minus strand synthesis), and then produce new plus
strands (transcripts) from the double-stranded RNA templates.
The three viral genomic segments contain unique packaging
signals at their 5′ ends, and minus strand synthesis
initiation is dependent on the sequence at the 3′
end. Here we have constructed chimeric segments that have
the packaging signal from one segment and the minus strand
synthesis initiation signal from another segment. Using
purified recombinant polymerase complexes and single-stranded
chimeric and original RNA segments, we have analyzed the
packaging and replication regulation operating in in vitro
conditions. We show that the 5′ end of the L genome
segment in single-stranded form is needed to switch from
the packaging to the minus strand synthesis and the same
sequence is required in double-stranded form to switch
on plus strand synthesis. In addition we have constructed
deletions to the M segment to analyze the possible regulatory
role of the internal noncoding area of this segment.
Email your librarian or administrator to recommend adding this to your organisation's collection.