Lameness manifests itself during the movement of the animal or at rest, indicating a structural or functional disturbance in one or more limbs. Videographic analyses allow the qualitative and quantitative investigation of the movement, reducing the subjectivity of evaluations. The objective was to record the alterations in the movement of the head and limbs of horses in a lame and non-lame condition. Video cameras (60 Hz) and computational methods were used. Six Purebred Arabian colts were filmed on a treadmill, at walk and at trot, before and after induction of lameness. Lameness was induced in the left forelimb utilizing a transient lameness model. Markers were placed on the zygomatic process of the temporal bone, spiny process of the sixth thoracic and first lumbar vertebrae and on the proximal phalange. Nine strides were analysed. At walk and at trot, the animals demonstrated two vertical head movements per stride, while after induction of lameness only one head movement was observed per stride, where this movement was of greater amplitude. The head was shown to be in a more elevated position when the lame limb made first contact with the treadmill belt, which was not observed in the case of the healthy limbs. Only with trotting did the lame animals manifest a prolonged duration of the stance phase for all limbs. The lame animals had a longer support time, lifted the non-lame limbs and showed a shorter stride. Videographic analyses offered details of the alterations in the movement of horses, which are important in the diagnosis of lameness.