Graphene is a 2D carbon allotrope that has attracted significant attention because its properties have a wide range of applications. Graphene was deposited on the polycrystalline nickel substrate with a dimension of 0.10 mm × 10 mm × 10 mm via thermal chemical vapor deposition (TCVD). The natural carbon source was obtained from a commercial palm oil as a carbon precursor. The D, G, and 2D bands described the vibration of graphitic layer and overtone of the D band at 1352, 1594, and 2716 cm−1, respectively. The lowest G band full width at half maximum (FWHM) was 38.7 cm−1 at 900 °C deposition temperature. In the x-ray diffraction (XRD) pattern, the FWHM of Ni (200) peak was 0.38°. Raman spectroscopy, UV–vis spectrophotometry, atomic force microscopy, XRD, and field emission scanning electron microscopy characterized the synthesized graphene. Multilayer graphene was successfully synthesized from the palm oil via TCVD.