Rhizoma perennial peanut (RPP) is well adapted to the Gulf Coast region of the United States, but its varietal tolerance to glyphosate and triclopyr is not well defined. The research was conducted to determine the effect of various rates of glyphosate and triclopyr on established RPP, and the response of common RPP varieties to these herbicides. The RPP sward was approximately 7 yr younger at Zolfo Springs than at the Ona location. RPP showed moderate tolerance to glyphosate and triclopyr application, and injury level did not differ with the age of RPP sward. However, biomass production was negatively influenced by the age of the RPP sward. Overall, injury from glyphosate applications did not exceed 40% at either site. The glyphosate rate for 20% biomass reduction was predicted to be 0.53 and 2.17 kg ae ha−1 at Zolfo Springs and Ona, respectively. RPP injury from triclopyr was greater at the Zolfo Springs location than at Ona, and the triclopyr rate predicted to result in a 20% biomass reduction was 0.45 and 0.99 kg ae ha−1 at the Zolfo Springs and Ona locations, respectively. There was a difference on RPP varieties response to glyphosate and triclopyr application. ‘Florigraze’ and ‘Ona 33’ were less tolerant to glyphosate compared to ‘UF-Tito’ and ‘Ecoturf’ at 30 d after treatment. Likewise, UF-Tito and Florigraze were less tolerant to triclopyr compared to Ona 33 and Ecoturf. Overall, Florigraze showed highest injury and at least 2-fold reduction on biomass compared to the other three varieties from glyphosate or triclopyr application. Results from this research indicate that glyphosate and triclopyr appear to be safe to apply to long-established RPP stands, but herbicide rate and RPP varieties should be considered if stands are <5 yr old.