Recent advances in recombinant DNA technology have created the potential for engineering of protein molecules to specific uses beyond those normally considered for biomaterials. This research project has demonstrated the feasibility of producing polypeptides useful for narrow band filters and nonlinear optical applications.
Synthetic genes, ranging in size from 36 to 576 base pairs, have been constructed from oligonucleotides using a restriction doubling technique. The synthetic genes have been inserted into a Protein A fusion expression system. Fused polypeptides from induced cells have been purified by affinity chromatography (IGG), and analyzed by polyacrylamide gel electrophoresis.