In this study, a dual-layered polarization and frequency reconfigurable microstrip antenna is proposed based on sequential mechanical axial rotation of the circular metal radiator. The antenna can be reconfigured among three different polarized modes, including the linear polarization (LP), left-handed circular polarization and right-handed circular polarization in the band from 4.68 to 4.80 GHz (2.53%). The resonance frequency of the proposed antenna with the same LP mode could also be tuned in the range from 4.70 to 5.03 GHz by mechanical rotation of the breach-truncated circular metal radiator as well as the circular substrate. Furthermore, the polarization characteristic and frequency can be reconfigured, respectively, as the circular radiator is taken an axial rotation with an angle of 360°. The presented antenna in the four different states has been numerically simulated and fabricated for the experimental measurement, the investigated characteristics includes the port reflection coefficient, axial ratio, radiation pattern, gain, and the radiation efficiency. The simulated and test results agreed well with each other. This antenna enriches the novel mechanical reconfigurable method except for the popular electrical approach.