Fossil insects from the late-glacial deposits at the Lamb Spring archaeological site, near Denver, Colorado, are relatively abundant and diverse, providing considerable paleoecological data for the site. The late Pleistocene insect fauna from the site comprises 72 identified taxa, principally beetles. However, the fauna presented an interpretive problem because it contained a mixture of prairie and alpine tundra species. This was initially considered to be the result of a mixing of faunal elements during the climatic transition of late-glacial times, a “no-modern-analog” fauna. Accelerator dating of insect fossil specimens from the two ecological groups helped resolve the paleoecological problem. Fossil specimens of the prairie-associated species were dated at 17,850 ± 550 yr B.P., while specimens of the tundra-associated species yielded an age of 14,500 ± 500 yr B.P. These dates reveal that what appeared to be an ecological mixing was probably a taphonomic problem, wherein full-glacial-age fossils were probably reworked into latest Wisconsin sediments. While both faunal assemblages reflect climatic conditions substantially colder than present, initial results suggest that the full-glacial fauna represents a cold, dry grassland or steppe environment, while the younger fauna suggests moister and more tundra-like conditions.