Utilizing magnetostrictive effect, the metallic glass is used to form mechanical resonators with different configurations. The resonance behaviors of these resonators are studied under different conditions, including different dc magnetic bias fields and different ac magnetic driving field. It is found that the resonators made of metallic glass exhibit a higher quality merit factor. Based on the results, it is also found that the acoustic wave velocity of the metallic glass decreases with increasing frequency. The application of these resonators as sensor platform is investigated. It is found that both odd and even vibration modes can be detected. Therefore, it provides a unique device that is capable to detect the target species on the sensor surface without “blind point(s)”, which is a challenge for all sensors based on other types of resonators. For the biosensors based on these resonators, a high sensitivity was observed. The advantages of these sensors over the current devices are demonstrated by the detection of Salmonella typhimurium (S. typhimurium) in water.