To investigate the prevalence of Cyclospora cayetanensis in a longitudinal study and to conduct a population genetic analysis, fecal specimens from 6579 patients were collected during the cyclosporiasis – prevalent seasons in two urban areas of central China in 2011–2015. The overall incidence of C. cayetanensis infection was 1·2% (76/6579): 1·6% (50/3173) in Zhengzhou and 0·8% (26/3406) in Kaifeng (P < 0·05), with infections in all age groups (P > 0·05). All the isolates clustered in the C. cayetanensis clade based on the small subunit ribosomal RNA gene sequence phylogenetic analysis. There were 45 specimens positive for all the five C. cayetanensis microsatellite loci, and formed 29 multilocus genotypes (MLGs). The phylogenetic relationships of 54 distinct MLGs (including 25 known reference MLGs), based on the concatenated multilocus sequences, formed three main clusters. A population structure analysis showed that the 79 isolates (including 34 known reference isolates) of C. cayetanensis produced three distinct subpopulations based on allelic profile data. In conclusion, we determined the frequency of C. cayetanensis infection in humans in Henan Province. The clonal population structure of the human C. cayetanensis isolates showed linkage disequilibrium and three distinct subpopulations.

The effects of high-Z dopant on the laser-driven ablative Richtmyer–Meshkov instability (RMI) are investigated by theoretical analysis and radiation hydrodynamics simulations. It is found that the oscillation amplitude of ablative RMI depends on the ablation velocity, the blow-off plasma velocity and the post-shock sound speed. Owing to enhancing the radiation at the plasma corona and increasing the radiation temperature at the ablation front, the high-Z dopant in plastic target can significantly increase the ablation velocity and the blow-off plasma velocity, leading to an increase in oscillation frequency and a reduction in oscillation amplitude of the ablative RMI. The high-Z dopant in plastic target is beneficial to reduce the seed of ablative Rayleigh–Taylor instability. These results are helpful for the design of direct drive inertial confinement fusion capsules.

We study the effects of Dy, Nb, and Ga additions on the microstructure and magnetic properties of Nd2Fe14B/α-Fe nanocomposites. Dy, Nb, and Ga additions inhibit the growth of the soft magnetic α-Fe phase. Dy and Nb additions are able to refine the microstructure, whereas Ga addition plays only a minor role in prohibiting crystal growth. The magnetic properties are sensitive to Dy, Nb, and Ga additions. The Dy-containing alloy enhances the intrinsic coercivity of 872 kA/m because Dy partially replaces Nd, forming (Nd, Dy)2Fe14B. Nb addition refines the microstructure, and consequently increases the exchange coupling between magnetic grains. The Nd9.5Fe75.4Co5Zr3B6.5Ga0.6 alloy exhibits the highest remanence (0.92 T) due to Ga addition.

The study reported in this Regional Research Communication aimed to analyse the genetic polymorphisms of β-casein in Chinese Holstein cows. β-casein has received considerable research interest in the dairy industry and animal breeding in recent years as a source not only of high quality protein, but also of bioactive peptides that may be linked to health effects. Morever, the polymorphic nature of β-casein and its association with milk production traits, composition, and quality also attracted several efforts in evaluating the allelic distribution of β-casein locus as a potential dairy trait marker. However, few data on beta-casein variants are available for the Chinese Holstein cow. In the present paper, one hundred and thirty three Holstein cows were included in the analysis. Results revealed the presence of 5 variants (A1, A2, A3, B and I), preponderance of the genotype A1A2 (0·353) and superiorities of A1/A2 alleles (0·432 and 0·459, respectively) in the population. Sequence analysis of β-casein gene in the cows showed four nucleotide changes in exon 7. Our study can provide reference and guidance for selection for superior milk for industrial applications and crossbreeding and genetic improvement programmes.

Induced pluripotent stem (iPS) cells have great potential in regenerative medicine, including cell replacement therapies and disease modelling in vitro. However, with this potential comes several challenges, including clinical safety, reprogramming and differentiation efficiency, and compromised functionality of differentiated cell types after transplantation. Many of these issues arise from imprecise control of cell fate. With large-scale sequencing and genome-editing technologies we can now precisely manipulate the genome, which has expanded our knowledge of functional cell types and cell identity. These technologies may improve our efforts in generating iPS-derived therapeutic cells and in development of therapies for human diseases.

Direct coagulation casting of alumina via controlled release of high valence counter ions using ammonium polyphosphate (APP) chelate complex as the coagulating agent was proposed. APP chelate complex suspension was prepared from APP and calcium chloride. Calcium used as high valence counter ions was chelated by APP. The average particle size of the chelate complex is 0.13 μm with a narrow particle size distribution which is close to the size of alumina particles. Glycerol diacetate was used to tailor the pH value of the suspension by hydrolysis which produces acetic acid. The lowering of the pH value helps to decompose the chelate complex, and enhance to release the calcium chelated. It is indicated that the viscosity of the suspensions with the addition of APP chelate complex suspension and glycerol diacetate increases to approximately 20 Pa s after heating at 40–70 °C for 1.5–5 h, which is high enough to coagulate the suspension. 55 vol% alumina suspension with a addition of 3 vol% APP chelate complex suspension and 5 vol% glycerol diacetate treated at 60 °C could coagulate completely within 2 h with a compressive strength of 2.1 MPa. Dense alumina with a relative density of above 97% and a flexural strength of 388 ± 23 MPa can be prepared by this method from 55 vol% alumina suspensions without a burnout process.

This paper describes an optimized magnetic wheel solution for use in a novel grit-blasting robot intended to be used on the hulls of ships. The grit-blasting robot was designed for conducting surface operations on newly-built ships in dry yards. It can be adapted to curvatures of up to 0.833 m−1; can achieve a total payload of 120 kg and can also be steered. The proposed magnetic wheel solution for robots with such payloads and surface adaptability has not been seen in previous work.

As the magnetic force acting on a magnetic wheel is very sensitive to the working conditions, a mathematical model was built to derive the exact force requirements taking into account the mechanical structure of the robot and its disposition on the ship's hull. In this paper, the design of the wheels was optimized based on the model. Wheels were manufactured according to the optimized results and a prototype robot was constructed. The design was then validated using locomotion tests.

Hydroxytyrosol (HT) is a major polyphenolic compound found in olive oil with reported anti-cancer and anti-inflammatory activities. However, the neuroprotective effect of HT on type 2 diabetes remains unknown. In the present study, db/db mice and SH-SY-5Y neuroblastoma cells were used to evaluate the neuroprotective effects of HT. After 8 weeks of HT administration at doses of 10 and 50 mg/kg, expression levels of the mitochondrial respiratory chain complexes I/II/IV and the activity of complex I were significantly elevated in the brain of db/db mice. Likewise, targets of the antioxidative transcription factor nuclear factor erythroid 2 related factor 2 including p62 (sequestosome-1), haeme oxygenase 1 (HO-1), and superoxide dismutases 1 and 2 increased, and protein oxidation significantly decreased. HT treatment was also found to activate AMP-activated protein kinase (AMPK), sirtuin 1 and PPARγ coactivator-1α, which constitute an energy-sensing protein network known to regulate mitochondrial function and oxidative stress responses. Meanwhile, neuronal survival indicated by neuron marker expression levels including activity-regulated cytoskeleton-associated protein, N-methyl-d-aspartate receptor and nerve growth factor was significantly improved by HT administration. Additionally, in a high glucose-induced neuronal cell damage model, HT effectively increased mitochondrial complex IV and HO-1 expression through activating AMPK pathway, followed by the prevention of high glucose-induced production of reactive oxygen species and declines of cell viability and VO2 capacity. Our observations suggest that HT improves mitochondrial function and reduces oxidative stress potentially through activation of the AMPK pathway in the brain of db/db mice.

Understanding uncertainty in estimating risk measures is important in modern financial risk management. In this paper we consider a nonparametric framework that incorporates auxiliary information available in covariates and propose a family of inferential methods for the value at risk, expected shortfall, and related risk measures. A two-step generalized empirical likelihood test statistic is constructed and is shown to be asymptotically pivotal without requiring variance estimation. We also show its validity when applied to a semiparametric index model. Asymptotic theories are established allowing for serially dependent data. Simulations and an empirical application to Canadian stock return index illustrate the finite sample behavior of the methodologies proposed.

Writing in language tests is regarded as an important indicator for assessing language skills of test takers. As Chinese language tests become popular, scoring a large number of essays becomes a heavy and expensive task for the organizers of these tests. In the past several years, some efforts have been made to develop automated simplified Chinese essay scoring systems, reducing both costs and evaluation time. In this paper, we introduce a system called SCESS (automated Simplified Chinese Essay Scoring System) based on Weighted Finite State Automata (WFSA) and using Incremental Latent Semantic Analysis (ILSA) to deal with a large number of essays. First, SCESS uses an n-gram language model to construct a WFSA to perform text pre-processing. At this stage, the system integrates a Confusing-Character Table, a Part-Of-Speech Table, beam search and heuristic search to perform automated word segmentation and correction of essays. Experimental results show that this pre-processing procedure is effective, with a Recall Rate of 88.50%, a Detection Precision of 92.31% and a Correction Precision of 88.46%. After text pre-processing, SCESS uses ILSA to perform automated essay scoring. We have carried out experiments to compare the ILSA method with the traditional LSA method on the corpora of essays from the MHK test (the Chinese proficiency test for minorities). Experimental results indicate that ILSA has a significant advantage over LSA, in terms of both running time and memory usage. Furthermore, experimental results also show that SCESS is quite effective with a scoring performance of 89.50%.

To detect the expression of pro-fibrotic molecules, such as heat shock protein 47 (Hsp47), transforming growth factor-beta 1 (TGF-β1) and connective tissue growth factor (CTGF) in liver specimens, and analyse their correlations with the progression of schistosomal hepatic fibrosis, liver biopsy was performed in 42 chronic schistosomiasis (CS) patients, 16 chronic hepatitis B (CHB) patients and five healthy individuals (HI). Immunohistochemistry (IHC) analyses displayed that the expression of Hsp47, TGF-β1 and CTGF was increased in CS and CHB patients compared with HI. Using real-time PCR, the mRNA levels of Hsp47, TGF-β1 and CTGF were higher in CS patients compared with HI. In CS patients, the mRNA levels of these genes were correlated with the stage of fibrosis, and TGF-β1 mRNA expression was associated with the grade of inflammation. Additional analyses indicated that the mRNA levels of Hsp47 and CTGF were highly correlated with liver stiffness value and spleen thickness diameter, both of which represented the severity of fibrosis. In conclusion, the three molecules are involved in the pathogenesis of hepatic fibrosis infected by Schistosoma japonicum. TGF-β1 participates not only in the inflammatory process, but also in the fibrotic process in which Hsp47 and CTGF probably play a key role.

This paper considers model-free hypothesis testing and confidence interval construction for conditional quantiles of time series. A new method, which is based on inversion of the smoothed empirical likelihood of the conditional distribution function around the local polynomial estimator, is proposed. The associated inferential procedures do not require variance estimation, and the confidence intervals are automatically shaped by data. We also construct the bias-corrected empirical likelihood, which does not require undersmoothing. Limit theories are developed for mixing time series. Simulations show that the proposed methods work well in finite samples and outperform the normal confidence interval. An empirical application to inference of the conditional value-at-risk of stock returns is also provided.

Reprogramming of DNA methylation in somatic cell nuclear transfer (SCNT) embryos is incomplete, and aberrant DNA methylation patterns are related to the inefficiency of SCNT. To facilitate nuclear reprogramming, this study investigated the effect of treating Guangxi Bama minipig donor cells with trichostatin A (TSA), 5-aza-2′-deoxycytine (5-aza-dC), or combination of TSA and 5-aza-dC prior to nuclear transfer. Analyses showed that there were no major changes in cell-cycle status among all groups. We monitored the transcription of DNMT1, DNMT3a, HDAC1 and IGF2 genes in donor cells. Transcription levels of HDAC1 were decreased significantly after treatment with a combination of TSA and 5-aza-dC, along with a significantly increased level of IGF2 (P < 0.05). Although treatment of donor cells with either TSA or 5-aza-dC alone resulted in non-significant effects in blastocyst formation rate and DNA methylation levels, a combination of TSA and 5-aza-dC significantly improved the development rates of minipig SCNT embryos to blastocyst (25.6% vs. 16.0%, P < 0.05). This change was accompanied by decreased levels of DNA methylation in somatic cells and blastocyst (P < 0.05). Thus in combination with TSA, lower concentrations of 5-aza-dC may produce a potent demethylating activity, and lead to the significantly enhanced blastocyst development percentage of Bama minipig SCNT embryos.

Dietary isothiocyanates have been shown to possess anti-tumour activity, inhibiting several types of cultured human cancer cell growth. However, there are limited studies on their effects on cancer cell metastasis. Our previous study showed that benzyl isothiocyanate (BITC) and phenethyl isothiocyanate (PEITC) suppressed human lung cancer cell metastasis potential. In the present study, we found BITC (7·5 and 10 μm) and PEITC (12·5 and 20 μm) induced highly metastatic human non-small cell lung cancer L9981 cell apoptosis in a dose-dependent manner. Caspase-3 was activated. They also caused cell cycle arrest at the G2/M phase, via modulation of cyclin B1 expression. The mitogen-activated protein kinase (MAPK) signalling pathway was involved. c-Jun N-terminal kinase, extracellular signal-regulated protein kinase 1/2 and p38 were activated in a dose-dependent manner; activator protein 1 (AP-1) transcriptional activation and cyclin D1 expression were repressed. Apoptosis and MAPK activation were abrogated by anti-oxidant N-acetyl cysteine (NAC), suggesting that cell death signalling was triggered by oxidative stress. Further microarray analysis evaluated the potential targeted genes related to apoptosis and the cell cycle. Our studies suggested that BITC and PEITC suppressed the metastasis potential of highly metastatic lung cancer cells by inducing apoptosis and cell cycle arrest, via targeting the MAPK/AP-1 pathway. This may provide a novel approach for metastasis therapy of lung cancer by dietary isothiocyanates and possibly other types of cancer.

We report the heterogeneous integration of a multifunctional sensor based on polymer porous photonic bandgap (P3BG) structure and xerogel based luminescence sensor technology. The P3BG structure was fabricated using holographic interferometry. Initially, holographic interferometry of a photo-activated prepolymer syrup that included a volatile solvent as well as monomer, photoinitiator, and co-initiator was used to initiate photopolymerization. Subsequent UV curing resulted in well defined lamellae of the polymer separated by porous polymer regions that created a high quality photonic bandgap structure. The resulting P3BG structure was then integrated with the xerogel based luminescence element to produce a luminescence sensor with a selective narrow band reflector. The prototype xerogel based luminescence sensor element consisted of an O2 sensing material based on spin coated tetraethylorthosilane (TEOS) composite xerogel films containing tris (4,7-diphenyl-1,10-phenanthroline) ruthenium (II) ([Ru(dpp)3]2+) luminophore. We demonstrated enhancement of the signal-to-noise ratio (SNR) of this integrated multifunctional sensor while maintaining the same sensitivity to O2 sensing of the xerogel based element. The resulting advantages and enhanced SNR of this integrated sensor will provide a template for other luminescence based assays to support highly sensitive and cost-effective sensor systems for biomedical applications.

The unloading of an indentation provides information about the shape of the indentation and the elastic properties of the materials. The assumptions of axisymmetry and material isotropy are critically examined, and a model for transversely isotropic materials is compared to measurements on single crystals. The methods used to infer the area of the indentation from the unloading curve are examined. The area is a fundamental value for the determination of hardness, modulus, and other mechanical properties in the so-called nano-indentor and other continuously monitored indentor techniques. The models of elastic recovery which are currently used are found to lack the flexibility to model the parameters which determine indentation depth. If the current self-consistent model is extended to cover the important aspects of the unloading, the area of the indentation is still not determined uniquely. Guidelines for further development of a unique model are suggested.

Cobalt-silver granular thin films were formed by Co implantation into Ag using a metal vapor vacuum arc (MEVVA) ion source. The magnetic field dependence and the temperature variation of the giant magnetoresistance (GMR) effect and their relation with the processing conditions were studied and discussed in conjunction with results of Rutherford backscattering spectrometry, atomic force microscopy, magnetic force microscopy (MFM), and SQUID measurements. Anomalous temperature dependence of the coercive field Hc in the perpendicular-to-film direction determined from GMR measurements was observed for some samples. For one sample, Hc shows a maximum value at around 240K and decreases with decreasing temperature from 240K to 20K. The temperature variation of the magnetization M of this sample exhibits a minimum. The maximum in the Hc-T curve corresponds well with the minimum in the M-T curve. The M-T curve suggests that there is more than one magnetic phase present in this sample. The domain structures of the implanted granular films as revealed by MFM images exhibit very different features compared with those of sputter deposited CoAg granular films.