Programmed cell death or apoptosis occurs in many tissues during
normal development and in the normal homeostasis of adult tissues.
Apoptosis also plays a significant role in abnormal development
and disease. Increased interest in apoptosis and cell death
in general has resulted in the development of new techniques
and the revival of old ones. Each assay has its advantages and
disadvantages that can render it appropriate and useful for
one application, but inappropriate or difficult to use in another.
Understanding the strengths and limitations of the assays would
allow investigators to select the best methods for their needs.