The dimethylamine salt and isopropyl and isooctyl ester formulations of 2,4-dichlorophenoxyacetic acid (2,4-D) were applied at 0, ½, 1, 2, and 4 lb/A 21, 14, and 7 days before harvesting Bison hard red winter wheat. Treatment effects on yield, chemical composition, or milling and baking properties of the grain were either nil or of no practical significance. When treated with ½ or 1 lb/A, rates generally recommended for farm use, 2,4-D residues in the grain ranged from 0.04 to 0.27 ppm. As rates of the three formulations of 2,4-D were increased from ½ to 4 lb/A, increasing amounts of 2,4-D residues occurred in the grain and reached a maximum of 1.8 ppm when the isopropyl ester of 2,4-D was applied at 4 lb/A 14 days before harvest. Grain from plots treated 21 days before harvest had less 2,4-D residue than grain from plots treated 14 and 7 days before harvest.