Research was conducted in winter of 2000 and spring of 2001 to determine the extent of simazine-resistant annual bluegrass in Mississippi. Samples of annual bluegrass seed or mature plants were collected from 71 locations across the state and grown in the greenhouse. Four weeks after treatment with 22.4 kg ai/ha simazine (10× rate), samples from 31 of 71 locations (30 golf courses and one nongolf course) evaluated in the greenhouse had simazine-resistant annual bluegrass plants. Thus, 43% of the golf courses tested in the greenhouse had triazine-resistant annual bluegrass present. When natural field populations were treated with an equivalent simazine rate, simazine-resistant annual bluegrass plants were observed in 90% of the sites found to be resistant in the greenhouse screen.