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1 results

  • MicroRNA-212 targets SIRT2 to influence lipogenesis in bovine mammary epithelial cell line

  • Xubin Lu, Hailei Xia, Jingyi Jiang, Xin Xu, Mingxun Li, Zhi Chen, Yujia Sun, Huimin Zhang, Zhangping Yang
  • Journal:
    Journal of Dairy Research / Volume 87 / Issue 2 / May 2020
    Published online by Cambridge University Press:
    16 April 2020, pp. 232-238
    Print publication:
    May 2020

  • In this research paper we filter and verify miRNAs which may target silent information regulator homolog 2 (SIRT2) gene and then describe the mechanism whereby miRNA-212 might regulate lipogenic genes in mammary epithelial cell lines via targeting SIRT2. Bioinformatics analysis revealed that the bovine SIRT2 gene is regulated by three miRNAs: miR-212, miR-375 and miR-655. The three miRNAs were verified and screened by qRT-PCR, western blot, and luciferase multiplex verification techniques and only miR-212 was shown to have a targeting relationship with SIRT2. The results of co-transfecting miR-212 and silencing RNA (siRNA) showed that by targeting SIRT2, miR-212 can regulate the expression of fatty acid synthetase (FASN) and sterol regulatory element binding factor 1 (SREBP1) but not peroxisome proliferator-activated receptor gamma (PPARγ). Measurement of triglyceride (TAG) content showed that miR-212 increased the fat content of mammary epithelial cell lines. The study indicates that miR-212 could target and inhibit the expression of the SIRT2 gene to promote lipogenesis in mammary epithelial cell lines.