Two studies evaluated the psychometric properties and the latent structure of the Portuguese version of the Penn State Worry Questionnaire (PSWQ) in a large Brazilian college student sample. Results indicated that PSWQ had an adequate internal consistency. Exploratory factor analyses yielded a two-factor solution. One factor was related to the worry presence and incorporated all the non-reversed items. The other factor was associated to worry absence and incorporated all the reversed items. Confirmatory factor analysis leaded to a three-factor solution. One factor included all the PSWQ items whereas the two other factors were linked to the reversed and non-reversed worded items. Correlations coefficients of these two reversed and non-reversed factors with the total scores of the PSWQ and the trait form of the State-Trait Anxiety Inventory suggest that PSWQ has a single meaningful construct.