The present paper describes the mineralization of calcium carbonate at a low temperature (4 °C) and in presence of additives. Aspartic acid immobilized polyacrylic acid (PA-Asp) induced the nucleation of spherical vaterite polymorph at 4 °C. But at room temperature, a thin film of calcite was deposited. Moreover, stable amorphous calcium carbonate was precipitated in presence of magnesium at low temperature. The observed results show that low temperature and presence of additives influence the nucleation, polymorph selectivity and morphology of the precipitated calcium carbonate.