Signalling and genetic networks underlie most biological processes and are often complex, containing many highly connected components. Modelling these networks can provide insight into mechanisms but is challenging given that rate parameters are often not well defined. Boolean modelling, in which components can only take on a binary value with connections encoded by logic equations, is able to circumvent some of these challenges, and has emerged as a viable tool to probe these complex networks. In this review, we will give an overview of Boolean modelling, with a specific emphasis on its use in plant biology. We review how Boolean modelling can be used to describe biological networks and then discuss examples of its applications in plant genetics and plant signalling.

Expression levels of genes (RT-qPCR) related to Ca and P homeostasis (transporters and claudins (CLDN)) were determined in porcine jejunal and colonic mucosa. Forty growing pigs (BW 30·4 (sem 1·3) kg) received a low and high Ca content (2·0 and 9·6 g/kg, respectively) diet with or without microbial phytase (500 FTU/kg) for 21 d. Dietary Ca intake enhanced serum Ca and alkaline phosphatase concentration and reduced P, 1,25(OH)2D3, and parathyroid hormone concentration. Jejunal transient receptor potential vanilloid 5 (TRPV5) mRNA expression was decreased (32%) with phytase inclusion only, while colonic TRPV5 mRNA was reduced by dietary Ca (34%) and phytase (44%). Both jejunal and colonic TRPV6 mRNA expression was reduced (30%) with microbial phytase. Calbindin-D9k mRNA expression was lower in colonic but not jejunal mucosa with high dietary Ca (59%) and microbial phytase (37%). None of the mRNAs encoding the Na–P cotransporters (NaPi-IIc, PiT-1, PiT-2) were affected. Jejunal, but not colonic expression of the phosphate transporter XPR1, was slightly downregulated with dietary Ca. Dietary Ca downregulated colonic CLDN-4 (20%) and CLDN-10 (40%) expression while CLDN-7 was reduced by phytase inclusion in pigs fed low dietary Ca. Expression of colonic CLDN-12 tended to be increased by phytase. In jejunal mucosa, dietary Ca increased CLDN-2 expression (48%) and decreased CLDN-10 (49%) expression, while phytase slightly upregulated CLDN-12 expression. In conclusion, compared with a Ca-deficient phytase-free diet, high dietary Ca and phytase intake in pigs downregulate jejunal and colonic genes related to transcellular Ca absorption and upregulate Ca pore-forming claudins.

Over the last two decades, international humanitarian law (IHL) has seen a stalling with regard to States’ willingness to adopt treaties or to be formally involved in the development of IHL. This raises the question of whether holding on to the doctrine of sources as laid down in Article 38 of the Statute of the International Court of Justice is the only way to meaningfully further develop IHL. Indeed, in recent years IHL instruments have often dispensed with certain formalities that are traditionally linked to (the formal sources of) international law; this phenomenon is also called “informal international law-making” (IIL). The present contribution will analyze IIL as an alternative way forward in light of the current “deadlock” caused by States’ unwillingness to conclude new IHL treaties or to recognize customary IHL. In this article, we will investigate and assess the opportunities, shortcomings and pitfalls offered by informality by looking into examples of IIL within IHL. More concretely, we will look into State practice in relation to (1) the Safe Schools Declaration, (2) the Tallinn Manual and Tallinn Manual 2.0, and (3) the Montreux Document. Most importantly, our findings will assess whether IIL can overcome one of its alleged main disadvantages: its lack of effectiveness.

Sixty growing male pigs were used to test the hypothesis that high dietary Ca content reduces P absorption to a greater extent in microbial phytase-supplemented diets via reducing inositol phosphate (IP) degradation and enhancing P precipitation. Pigs were equally allotted over diets with three Ca contents 2·0, 5·8 and 9·6 g/kg with or without microbial phytase (0 v. 500 FTU/kg) in a 2 × 3 factorial arrangement. Faeces and urine were collected at the end of the 21-d experimental period. Subsequently, pigs were euthanised and digesta quantitatively collected from different gastrointestinal tract (GIT) segments. Increasing dietary Ca content reduced apparent P digestibility in all GIT segments posterior to the stomach (P < 0·001), with greater effect in phytase-supplemented diets in the distal small intestine (P interaction = 0·007) and total tract (P interaction = 0·023). Nonetheless, increasing dietary Ca to 5·8 g/kg enhanced P retention, but only in phytase-supplemented diets. Ileal IP6 degradation increased with phytase (P < 0·001) but decreased with increasing dietary Ca content (P = 0·014). Proportion of IP esters in total IP (∑IP) indicated that IP6/∑IP was increased while IP4/∑IP and IP3/∑IP were reduced with increasing dietary Ca content and also with a greater impact in phytase-supplemented diets (P interaction = 0·025, 0·018 and 0·009, respectively). In all GIT segments, P solubility was increased with phytase (P < 0·001) and tended to be reduced with dietary Ca content (P < 0·096). Measurements in GIT segments showed that increasing dietary Ca content reduced apparent P digestibility via reducing IP degradation and enhancing P precipitation, with a greater impact in phytase-supplemented diets due to reduced IP degradation.

This paper, written by Gineke Wiggers, Suzan Verberne, Gerrit-Jan Zwenne and Wouter Van Loon, addresses the concept of ‘relevance’ in relation to legal information retrieval (IR). They investigate whether the conceptual framework of relevance in legal IR, as described by Van Opijnen and Santos in their paper published in 2017, can be confirmed in practice.1 The research is conducted with a user questionnaire in which users of a legal IR system had to choose which of two results they would like to see ranked higher for a query and were asked to provide a reason for their choice. To avoid questions with an obvious answer and extract as much information as possible about the reasoning process, the search results were chosen to differ on relevance factors from the literature, where one result scores high on one factor, and the other on another factor. The questionnaire had eleven pairs of search results. A total of 43 legal professionals participated consisting of 14 legal information specialists, 6 legal scholars and 23 legal practitioners. The results confirmed the existence of domain relevance as described in the theoretical framework by Van Opijnen and Santos as published in 2017.2 Based on the factors mentioned by the respondents, the authors of this paper concluded that document type, recency, level of depth, legal hierarchy, authority, usability and whether a document is annotated are factors of domain relevance that are largely independent of the task context. The authors also investigated whether different sub-groups of users of legal IR systems (legal information specialists who are searching for others, legal scholars and also for legal practitioners) differ in terms of the factors they consider in judging the relevance of legal documents outside of a task context. Using a PERMANOVA there was found to be no significant difference in the factors reported by these groups. At this moment there is no reason to treat these sub-groups differently in legal IR systems.

The European Union (EU or Union) has the ambition to be an important actor in the global order. Pursuant to Article 21 of the Treaty on European Union (TEU), it is set to “work for a high degree of cooperation in all fields of international relations” and “promote an international system based on stronger multilateral cooperation and good global governance.” More specifically, as TEU Article 3(5) stipulates, “[i]n its relations with the wider world,” the EU is tasked to “contribute to peace, security, the sustainable development of the Earth, solidarity and mutual respect among peoples, free and fair trade, eradication of poverty and the protection of human rights, in particular the rights of the child, as well as to the strict observance and the development of international law, including respect for the principles of the United Nations Charter.”

The corporate responsibility to respect human rights was formally introduced in 2011 with the unanimous endorsement of the UN Guiding Principles on Business and Human Rights (UNGPs) by the UN Human Rights Council. It is grounded in social expectations and forms part of the companies' “social license to operate.” This paper argues that this responsibility is progressively turning into a legal duty for lead companies to respect human rights in those types of value chains which are characterized by a high level of control by a lead company over its business partners. Our argument rests on two recent legal developments. Firstly, the article analyzes the judicialization of the corporate responsibility to respect in the case law on parent company liability in various jurisdictions, which, we argue, is highly likely to have some implications in relation to certain types of value chains so as to trigger the liability of lead companies for the human rights harms arising out of the activities of entities over which they exercise sufficient control. Secondly, the article delves into the legislative developments which increasingly require lead companies to exercise due diligence so as to prevent and address adverse human rights impacts in their own activities and global value chains.

Through the observations and the analysis of maser polarization it is possible to measure the magnetic field in several astrophysical environments (e.g., star-forming regions, evolved stars). In particular from the linearly and circularly polarized emissions we can determine the orientation and the strength of the magnetic field, respectively. In these proceedings the implications, on observed data, of the new estimation of the Landé g-factors for the CH3OH maser are presented. Furthermore, some example of the most recent results achieved in observing the polarized maser emission from several maser species will also be reported.

Maser observations of both linearly and circularly polarized emission have provided unique information on the magnetic field in the densest parts of star forming regions, where non-maser magnetic field tracers are scarce. While linear polarization observations provide morphological constraints, magnetic field strengths are determined by measuring the Zeeman splitting in circularly polarized emission. Methanol is of special interest as it is one of the most abundant maser species and its different transitions probe unique areas around the protostar. However, its precise Zeeman-parameters are unknown. Experimental efforts to determine these Zeeman-parameters have failed. Here we present quantum-chemical calculations of the Zeeman-parameters of methanol, along with calculations of the hyperfine structure that are necessary to interpret the Zeeman effect in methanol. We use this model in re-analyzing methanol maser polarization observations. We discuss different mechanisms for hyperfine-state preference in the pumping of torsion-rotation transitions involved in the maser-action.

This article explores the idea that the USDOC imposed anti-dumping duties on Vietnamese shrimp producers despite the fact that the surge of shrimp imports giving rise to the duties may have come from elsewhere in the developing world. We argue that Vietnam's shrimp exporters may have been subject to anti-dumping duties because Vietnam has ‘non-market economy’ (NME) status in the United States. This makes it possible to levy higher duties against Vietnamese firms. We make the point that it was particularly inappropriate to impose anti-dumping duties against the Vietnamese shrimp industry because this industry shows clear indications of being perfectly competitive, whereby firms cannot dump. This in turn raises the question of how the USDOC was able to construct a dumping case where apparently none could have existed. Use of the ‘zeroing’ methodology, in conjunction with Vietnam's NME status, turns out to be central to the answer. The broader issue is that anti-dumping duties are overused where safeguards would be more efficient. The analysis is relevant for the current controversy over China's NME status with a number of its trading partners.