The effects of twelve soil metabolites of trifluralin [2,6-dinitro-N,N-dipropyl-4-(trifluoromethyl)benzenamine] on soybean [Glycine max (L.) Merr.] growth, yield, and seed oil and protein content were determined. The metabolites included oxidative dealkylated, reduced nitro group, benzimidazole, azoxy, azo, oxidized, and hydroxylated derivatives of trifluralin. When applied at 0.22 kg ai/ha and incorporated 7.5 cm deep, no individual metabolite had a significant adverse effect on growth or yield. Three of the metabolites significantly decreased seed oil content and two of the metabolites significantly increased seed protein content. These effects are not considered important in that the differences, while statistically significant, are very small. Also, the amounts of metabolites applied greatly exceeded those expected to be found after many years of continuous use of trifluralin. Potential accumulation of individual metabolites from long-term use of trifluralin does not appear to adversely affect soybean growth, yield, or quality.