We report the molecular characterization of a middle repetitive
sequence, named C6, isolated from the Trypanosoma
cruzi genome. C6 appears to be a composite repeated element since
subregions may be defined within it on the basis
of sequence similarities with other T. cruzi genomic sequences.
Sequences homologous to C6 are interspersed in the
genome and can be mapped out on most chromosomal bands of different
T. cruzi strains. The copy number of the C6
element is about 1000 per haploid genome. Given the species specificity
different genomic distribution of C6
homologous sequences among the T. cruzi strains the C6 element
be a useful probe for diagnosis and typing of
parasites. C6 is a polymorphic marker with potential as a tool for
physical mapping of the T. cruzi genome.