Large-scale coherent structures in incompressible turbulent pipe flow are studied for a wide range of Reynolds numbers ($Re_\tau =180, 550, 1000, 2000$ and $5200$). Employing the Karhunen–Loève decomposition and a novel approach based on the Voronoi diagram, we identify and classify statistically coherent structures based on their location, dimensions and $Re_{\tau }$. With increasing $Re_{\tau }$, two distinct classes of structures become more energetic, namely wall-attached and detached eddies. The Voronoi methodology is shown to delineate these two classes without the need for specific criteria or thresholds. At the highest $Re_{\tau }$, the attached eddies scale linearly with the wall-normal distance with a slope of approximately $l_y\sim 1.2y/R$, while the detached eddies remain constant at the size of $l_y \approx 0.26R$, with a progressive shift towards the pipe centre. We extract these two classes of structures and describe their spatial characteristics, including radial size, helix angle and azimuthal self-similarity. The spatial distribution could help explain the differences in mean velocity between pipe and channel flows, as well as in modelling large and very-large-scale motions (LSM and VLSM). In addition, a comprehensive description is provided for both wall-attached and detached structures in terms of LSM and VLSM.

The term ‘chrono-nutrition’ describes the interaction between circadian timing and nutritional intake, as demonstrated by a wealth of animal and human studies(1). This relationship is bidirectional, with post-prandial response varying across the day and meal timing acting as a synchronising signal for some circadian rhythms. In humans, we have demonstrated that meal timing is a strong synchroniser of plasma glucose rhythms(2). Our recent data also suggest that interstitial glucose concentration can anticipate large meals(3). However, little is known about the effects of meal timing on other metabolic pathways. The existence of circadian rhythms in human plasma metabolites(4-6) provides a clear opportunity to address this timely issue. Here, in a controlled laboratory protocol, we tested the hypothesis that the anticipation of large meals is observed in human plasma metabolites.

Twenty-four male participants undertook an 8-day laboratory study, with strict sleep-wake schedules, light-dark schedules, and food intake. For 6 days, participants consumed either hourly small meals throughout the waking period or two large daily meals (7.5 and 14.5 h after wake-up). Isocaloric meals were calculated using the Mifflin St Jeor formula, and contained 55% carbohydrate, 15% protein, 30% fat(2). All participants then undertook a 37-h constant routine. Samples were collected across a 30-h period in the middle of the constant routine. Plasma was collected every 30 minutes for targeted UPLC-MS/MS metabolomics analysis (Absolute p180 Biocrates kit), and saliva was collected hourly for assessment of melatonin onset, a marker of circadian timing. Data were statistically evaluated by cosinor analysis, unpaired t-test, and repeated measures ANOVA.

There was no difference (p >0.05) in melatonin onset between the two groups. Rhythms, as indicated by significant (p < 0.05) cosinor fit, were detected in 64 plasma metabolites (out of 141 detected) in both groups. These metabolites included glucose, triglyceride, cholesterol, plus multiple amino acids, acylcarnitines, phospholipids and sphingolipids. Consistent with previous work, there was a large (c. 7-hour) difference in the phase of plasma glucose between groups, with no significant difference in the phase of plasma triglyceride. Some other metabolites (e.g. histidine and proline) exhibited phase changes like that of glucose, but the phase was mostly unchanged. Glucose, alanine, arginine, citrulline, glutamine, methionine, phenylalanine, met-SO, 3 acylcarnitines and 13 phospholipids exhibited a significant interaction (FDR <0.05 meal-size x time, n=25). Totalcholesterol, asparagine, glutamate, glycine, isoleucine, leucine, ornithine, proline, serine, threonine, tryptophan, tyrosine, kynurenine, SDMA, sarcosine, t4-OH-Pro, taurine, 6 acylcarnitines, 25 phospholipids and SMC18:1 exhibited a significant effect of meal-size and time. A further 8 metabolites had a significant effect of time only. Sixteen metabolites had no significant differences in neither meal size nor time. Our data therefore reveal novel effects of meal timing on multiple metabolite rhythms and average daily concentrations.

We hypothesized that the incubation for urethral gonorrhoea would be longer for men with oropharyngeal gonorrhoea than those without oropharyngeal gonorrhoea. We conducted a chart review of men who have sex with men with urethral gonorrhoea symptoms at a sexual health clinic between 2019 and 2021. The incubation period was defined as the number of days between men’s last sexual contact and onset of symptoms. We used a Mann–Whitney U test to compare differences in the median incubation for urethral gonorrhoea between men with and men without oropharyngeal gonorrhoea. There were 338 men with urethral symptoms (median age = 32 years; IQR: 28–39), and of these, 307 (90.1%) were tested for oropharyngeal gonorrhoea, of whom 124 (40.4%, 95% CI: 34.9–46.1) men had oropharyngeal and urethral gonorrhoea. We analyzed incubation data available for 190 (61.9%) of the 307 men, with 38.9% (74/190) testing positive for oropharyngeal gonorrhoea. The incubation for urethral gonorrhoea did not differ between 74 men (39%) with oropharyngeal gonorrhoea (median = 4 days; IQR: 2–6) and 116 men (61%) without oropharyngeal gonorrhoea (median = 2.5 days; IQR: 1–5) (p = 0.092). Research is needed to investigate gonorrhoea transmission from the oropharynx to the urethra.

Maternal diet may modulate human milk microbiota, but the effects of nutritional supplements are unknown. We examined the associations of prenatal diet and supplement use with milk microbiota composition. Mothers reported prenatal diet intake and supplement use using self-administered food frequency and standardised questionnaires, respectively. The milk microbiota was profiled using 16S rRNA gene sequencing. Associations of prenatal diet quality, dietary patterns, and supplement use with milk microbiota diversity and taxonomic structure were examined using Wilcoxon signed-rank tests and multivariable models adjusting for relevant confounders. A subset of 645 mothers participating in the CHILD Cohort Study (originally known as the Canadian Healthy Infant Longitudinal Development Study) provided one milk sample between 2 and 6 months postpartum and used prenatal multivitamin supplements ≥4 times a week. After adjusting for confounders, vitamin C supplement use was positively associated with milk bacterial Shannon diversity (β = 0.18, 95% CI = 0.05, 0.31) and Veillonella and Granulicatella relative abundance (β = 0.54; 95% CI = 0.05, 1.03 and β = 0.44; 95% CI = 0.04, 0.84, respectively), and negatively associated with Finegoldia relative abundance (β = –0.31; 95% CI = –0.63, –0.01). Fish oil supplement use was positively associated with Streptococcus relative abundance (β = 0.26; 95% CI = 0.03, 0.50). Prenatal diet quality and dietary patterns were not associated with milk microbiota composition. Prenatal vitamin C and fish oil supplement use were associated with differences in the milk microbiota composition. Future studies are needed to confirm our findings and elucidate mechanisms linking maternal supplement use to milk microbiota and child health.

We examined the efficacy of cognitive and behavioral interventions for improving symptoms of depression and anxiety in adults with neurological disorders. A pre-registered systematic search of Cochrane Central Register of Controlled Trials, MEDLINE, PsycINFO, Embase, and Neurobite was performed from inception to May 2024. Randomized controlled trials (RCTs) which examined the efficacy of cognitive and behavioral interventions in treating depression and/or anxiety among adults with neurological disorders were included. Estimates were pooled using a random-effects meta-analysis. Subgroup analyses and meta-regression were performed on categorical and continuous moderators, respectively. Main outcomes were pre- and post-intervention depression and anxiety symptom scores, as reported using standardized measures. Fifty-four RCTs involving 5372 participants with 11 neurological disorders (including multiple sclerosis, epilepsy, stroke) were included. The overall effect of interventions yielded significant improvements in both depression (57 arms, Hedges' g = 0.45, 95% confidence interval [CI] 0.35–0.54) and anxiety symptoms (29 arms, g = 0.38, 95% CI 0.29–0.48), compared to controls. Efficacy was greater in studies which employed a minimum baseline symptom severity inclusion criterion for both outcomes, and greater in trials using inactive controls for depression only. There was also evidence of differential efficacy of interventions across the neurological disorder types and the outcome measure used. Risk of bias, intervention delivery mode, intervention tailoring for neurological disorders, sample size, and study year did not moderate effects. Cognitive and behavioral interventions yield small-to-moderate improvements in symptoms of both depression and anxiety in adults with a range of neurological disorders.

We present a detailed analysis of nearly two decades of optical/UV and X-ray data to study the multi-wavelength pre-explosion properties and post-explosion X-ray properties of nearby SN2023ixf located in M101. We find no evidence of precursor activity in the optical to UV down to a luminosity of $\lesssim$$1.0\times10^{5}\, \textrm{L}_{\odot}$, while X-ray observations covering nearly 18 yr prior to explosion show no evidence of luminous precursor X-ray emission down to an absorbed 0.3–10.0 keV X-ray luminosity of $\sim$$6\times10^{36}$ erg s$^{-1}$. Extensive Swift observations taken post-explosion did not detect soft X-ray emission from SN2023ixf within the first $\sim$3.3 days after first light, which suggests a mass-loss rate for the progenitor of $\lesssim$$5\times10^{-4}\,\textrm{M}_{\odot}$ yr$^{-1}$ or a radius of $\lesssim$$4\times10^{15}$ cm for the circumstellar material. Our analysis also suggests that if the progenitor underwent a mass-loss episode, this had to occur $>$0.5–1.5 yr prior to explosion, consistent with previous estimates. Swift detected soft X-rays from SN2023ixf $\sim$$4.25$ days after first light, and it rose to a peak luminosity of $\sim10^{39}$ erg s$^{-1}$ after 10 days and has maintained this luminosity for nearly 50 days post first light. This peak luminosity is lower than expected, given the evidence that SN2023ixf is interacting with dense material. However, this might be a natural consequence of an asymmetric circumstellar medium. X-ray spectra derived from merging all Swift observations over the first 50 days are best described by a two-component bremsstrahlung model consisting of a heavily absorbed and hotter component similar to that found using NuSTAR, and a less-absorbed, cooler component. We suggest that this soft component arises from cooling of the forward shock similar to that found in Type IIn SN2010jl.

Early life stress (ELS) and a Western diet (WD) promote mood and cardiovascular disorders, however, how these risks interact in disease pathogenesis is unclear. We assessed effects of ELS with or without a subsequent WD on behaviour, cardiometabolic risk factors, and cardiac function/ischaemic tolerance in male mice. Fifty-six new-born male C57BL/6J mice were randomly allocated to a control group (CON) undisturbed before weaning, or to maternal separation (3h/day) and early (postnatal day 17) weaning (MSEW). Mice consumed standard rodent chow (CON, n = 14; MSEW, n = 15) or WD chow (WD, n = 19; MSEW + WD, n = 19) from week 8 to 24. Fasted blood was sampled and open field test and elevated plus maze (EPM) tests undertaken at 7, 15, and 23 weeks of age, with hearts excised at 24 weeks for Langendorff perfusion (evaluating pre- and post-ischaemic function). MSEW alone transiently increased open field activity at 7 weeks; body weight and serum triglycerides at 4 and 7 weeks, respectively; and final blood glucose levels and insulin resistance at 23 weeks. WD increased insulin resistance and body weight gain, the latter potentiated by MSEW. MSEW + WD was anxiogenic, reducing EPM open arm activity vs. WD alone. Although MSEW had modest metabolic effects and did not influence cardiac function or ischaemic tolerance in lean mice, it exacerbated weight gain and anxiogenesis, and improved ischaemic tolerance in WD fed animals. MSEW-induced increases in body weight (obesity) in WD fed animals in the absence of changes in insulin resistance may have protected the hearts of these mice.

Synthetic Aperture Radar (SAR) has been used extensively to determine the surface ice flow velocity of tidewater glaciers and investigate changes in seasonal or annual ice dynamics at medium spatial resolution (⩾100 m). However, assessing tidewater glacier behaviour at these resolutions risks missing key details of glacier dynamics, which is particularly important for determination of strain rates that relate to crevasse formation, depth, and ice damage. Here we present surface ice velocity and strain maps with a 16 m posting derived from high-resolution (1 m) PAZ Ciencia spotlight mode SAR imagery for Narsap Sermia, SW Greenland, for October 2019 to February 2021. Results reveal fine details in strain rate, including an area of compression proximal to the terminus, with an upstream shift of strains through time. The velocity evolution of Narsap Sermia shows distinct seasonal changes starting in summer 2020, which are largely modulated by the subglacial drainage system. Comparison of our results with medium-resolution velocity products shows that while these can capture general strain and velocity patterns, our high-resolution data reveals considerably larger ranges of strain values. This is likely to have implications for tuning strain rate dependent calving and ice damage parameterisations within numerical models.

Dyads can be challenging to recruit for research studies, but detailed reporting on strategies employed to recruit adult–adolescent dyads is rare. We describe experiences recruiting adult–youth dyads for a hypertension education intervention comparing recruitment in an emergency department (ED) setting with a school-based community setting. We found more success in recruiting dyads through a school-based model that started with adolescent youth (19 dyads in 7 weeks with < 1 hour recruitment) compared to an ED-based model that started with adults (2 dyads in 17 weeks with 350 hours of recruitment). These findings can benefit future adult–youth dyad recruitment for research studies.

Physical vapor deposited (PVD) molybdenum disulfide (nominal composition MoS2) is employed as a thin film solid lubricant for extreme environments where liquid lubricants are not viable. The tribological properties of MoS2 are highly dependent on morphological attributes such as film thickness, orientation, crystallinity, film density, and stoichiometry. These structural characteristics are controlled by tuning the PVD process parameters, yet undesirable alterations in the structure often occur due to process variations between deposition runs. Nondestructive film diagnostics can enable improved yield and serve as a means of tuning a deposition process, thus enabling quality control and materials exploration. Grazing incidence X-ray diffraction (GIXRD) for MoS2 film characterization provides valuable information about film density and grain orientation (texture). However, the determination of film stoichiometry can only be indirectly inferred via GIXRD. The combination of density and microstructure via GIXRD with chemical composition via grazing incidence X-ray fluorescence (GIXRF) enables the isolation and decoupling of film density, composition, and microstructure and their ultimate impact on film layer thickness, thereby improving coating thickness predictions via X-ray fluorescence. We have augmented an existing GIXRD instrument with an additional X-ray detector for the simultaneous measurement of energy-dispersive X-ray fluorescence spectra during the GIXRD analysis. This combined GIXRD/GIXRF analysis has proven synergetic for correlating chemical composition to the structural aspects of MoS2 films provided by GIXRD. We present the usefulness of the combined diagnostic technique via exemplar MoS2 film samples and provide a discussion regarding data extraction techniques of grazing angle series measurements.

Beta-lactam therapeutic drug monitoring has been growing in prevalence in the acute care hospital setting. Expansion of its use to outpatient parenteral antimicrobial therapy requires careful consideration of potential logistical and therapeutic barriers.