Introduction. México is one
of the most important producers and exporters of blackberries in
the world. During postharvest handling, blackberry fruits are exposed
to the attack of phytopathogenic fungi. Materials and methods.
To obtain Rhizopus stolonifer isolates, samples
of leaves and rhizospheric soils were placed on Petri plates containing
Potato Dextrose Agar (PDA). In addition, fruits were also placed
in humidity chambers at 25 °C. To describe the infection process
on blackberry fruits by Rhizopus stolonifer, samples
were taken at different intervals for a period of 72 h. All fungal
isolates obtained were maintained on PDA. The bacterial colonies
were isolated and purified by streaking on PDA. The antagonistic
activity of the bacteria was assessed against R. stolonifer by
dual culture technique on PDA. To detect the production of siderophores,
the chrome azurol S assay was carried out. Results.
The infection process of R. stolonifer on blackberry
fruits was described for the first time in this work. Eighty-six
bacterial isolates from different parts of the plant and rhizospheric
soil were obtained. Bacterial isolates with antagonistic activity
were identified and the production of siderophores was measured.
Four isolates showed antagonistic activity against R. stolonifer.
Bacillus subtilis obtained from soil was the most
effective isolate. Conclusion. In this study we isolated
and identified antagonistic bacteria with potential for biocontrol
on Rhizopus stolonifer obtained from blackberry fruits.
To our knowledge this is the first report regarding this topic.